Catalytic Materials Enabled by a Programmable Assembly of Synthetic Polymers and Engineered Bacterial Spores

Kawada, Masamu; Jo, Hyuna; Medína, A.; Sim, Seunghyun

doi:10.1021/jacs.3c05153

Cited by 7 publications

(1 citation statement)

References 32 publications

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“…Functional components-anchored cell platforms have emerged as versatile tools for targeting a variety of lesions, such as engineered immune cells, stem cells, tumor cells, bacteria, and platelets. − Macrophages (Mø) is a type of phagocytes with strong migratory properties to remove foreign debris and cells from an injury site. − When the tissue becomes infected or damaged, inflammatory monocytes’ CC chemokines 2 (CCR2) interact with monocyte chemotactic protein (MCP-1), causing mononuclear cells to differentiate into macrophages and migrate to the injured area . Engineered macrophages have shown great potential in targeted drug delivery due to their inflammatory homing and immune escape capabilities. , Compared with live engineered macrophages or macrophage membranes, inactivated macrophages avoid the risk of exacerbating inflammation and highly preserve the complete cell membrane structures, thereby affording a safe and natural inflammation-targeted agent …”

Section: Introductionmentioning

confidence: 99%

Walking Dead Macrophage-Based Positive Enhancement MRI for Ultrahighly Efficient Diagnosis of Nephritis

Lin,

Wu,

Chen

et al. 2024

Anal. Chem.

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Nephritis is an inflammatory condition of the glomerulus, and the clinical gold standard for its diagnosis is a kidney biopsy. However, obtaining biopsy results can take several days, which does not meet the requirement of rapid diagnosis, especially for rapidly progressive types. To achieve an effective and noninvasive diagnosis, we propose a nephritis-specific, positive magnetic resonance imaging (MRI) contrast agent based on Gd 3+ anchored walking dead macrophage Gd-RAW. Gd-RAW exhibits high selectivity for inflammatory renal parenchyma and provides comparable results to histopathology methods. The Gd-RAWbased MRI contrast agent reduces the diagnostic time of nephritis from 14 days of biopsy to 1 h. Furthermore, in a unilateral nephritis model constructed by increasing the glycerol concentration, the T 1 WI of renal parenchyma exhibits an increased signal-to-noise ratio, which is crucial for evaluating nephritic severity. This work promotes rapid diagnosis of nephritis and potentially provides sufficient evidence for clinicians to offer timely treatment to patients. The methodology of paramagnetic ion-anchored macrophage corpse also opens up new prospects for designing more specific and biosafe MRI contrast agents.

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Section: Introductionmentioning

confidence: 99%

Walking Dead Macrophage-Based Positive Enhancement MRI for Ultrahighly Efficient Diagnosis of Nephritis

Lin,

Wu,

Chen

et al. 2024

Anal. Chem.

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Leveraging the Power of Enzymes in Engineered Dead and Living Materials

Shannon,

Zhou,

Perriman

2024

Adv Funct Materials

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Significant advances are being made in the incorporation of enzymes into functional materials, which leverage their inherent substrate specificity, efficient catalytic activity, and sustainable origin. These engineered “dead” materials, however, lack the incredible systems‐level control of enzyme activity that living organisms have evolved over millennia. This gap is now being bridged by the rapidly emerging field of engineered living materials (ELMs), which couples the tools of advanced synthetic biology with modern materials science. In this review, the impressive array of methodologies used to fabricate the extensive library of functional enzyme‐based dead materials is discussed, and the design strategies that facilitate their creation unpacked. The spectacular suite of natural and synthetic genetic and post‐translational control systems for enzymes in living organisms is then described. Finally, key recent examples of ELMs that utilize enzyme activity are reviewed, highlighting the central role of the living component in providing responsivity and adaptability to this new class of materials.

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Artificial Spores as Multi‐Functional Biocatalysts to Perform Biosynthetic Cascades

Iturralde,

Ripoll,

Silvio

et al. 2024

Adv Funct Materials

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Cells exhibit diverse structural formations such as biofilms and spores, enabling them to acquire novel functionalities. Many of these structures display biomacromolecules, including enzymes, tethered to cell walls to support various extracellular processes. Alternatively, encapsulating single cells with polymer coatings offers a strategy that circumvents the need for genetic engineering while imparting artificial functionalities to cells. Here, a universal method is presented for encapsulating single gram‐negative microbes with polymeric coatings based on the ancestral gall ink formed by tannic acid‐iron complexes. As a result, synthetic spores are achieved that selectively bind His‐tagged enzymes through the formation of unprecedented galloyl/imidazole‐Fe2+ complexes via ligand substitution demonstrated by density functional theory. These synthetic spores with a thickness of 41.5 ± 4.2 nm and a stiffness of 6.0 ± 3.5 GPa serve as biocatalytic materials for the one‐pot oxidative amination of diols into amino alcohols, facilitated by the cooperative catalysis between intracellular endogenous or recombinant oxidoreductases, and an extracellular transaminase from Pseudomonas fluorescens displayed at the spore surface. These spores maintain their performance in three consecutive batch cycles. Integrating isolated enzymes onto the surface of engineered microbes coated with polymers offers novel opportunities for synthetic biology, advancing the efficiency of biosynthetic cascades in solid‐state environments.

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Catalytic Materials Enabled by a Programmable Assembly of Synthetic Polymers and Engineered Bacterial Spores

Cited by 7 publications

References 32 publications

Walking Dead Macrophage-Based Positive Enhancement MRI for Ultrahighly Efficient Diagnosis of Nephritis

Walking Dead Macrophage-Based Positive Enhancement MRI for Ultrahighly Efficient Diagnosis of Nephritis

Leveraging the Power of Enzymes in Engineered Dead and Living Materials

Artificial Spores as Multi‐Functional Biocatalysts to Perform Biosynthetic Cascades

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