Catalytic Nucleic Acids for Bioanalysis

Mao, Xiuhai; Li, Qian; Zuo, Xiaolei; Chen, Fan

doi:10.1021/acsabm.9b00928

Cited by 20 publications

(16 citation statements)

References 183 publications

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“…Based on its specific recognition performance and ability to regulate DNA conformation, recent research efforts have emerged to produce bifunctional biomolecular structures (aptazymes) by combining DNAzymes with aptamers, in which the aptamer act as binding sites and the DNAzyme act as catalytic site. [ 180 ] The aptazyme has multiple response sites, so it becomes an ideal platform for the construction of multiple stimuli‐responsive system. For example, aptazyme with enhanced catalytic activities has been constructed while the dopamine aptamer act as a binding site, and the DNAzyme act as the catalytic unit.…”

Section: Dna Assembly‐based Stimuli‐responsive Dna Systemsmentioning

confidence: 99%

DNA Assembly‐Based Stimuli‐Responsive Systems

Fan

et al. 2021

Advanced Science

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Stimuli-responsive designs with exogenous stimuli enable remote and reversible control of DNA nanostructures, which break many limitations of static nanostructures and inspired development of dynamic DNA nanotechnology. Moreover, the introduction of various types of organic molecules, polymers, chemical bonds, and chemical reactions with stimuli-responsive properties development has greatly expand the application scope of dynamic DNA nanotechnology. Here, DNA assembly-based stimuli-responsive systems are reviewed, with the focus on response units and mechanisms that depend on different exogenous stimuli (DNA strand, pH, light, temperature, electricity, metal ions, etc.), and their applications in fields of nanofabrication (DNA architectures, hybrid architectures, nanomachines, and constitutional dynamic networks) and biomedical research (biosensing, bioimaging, therapeutics, and theranostics) are discussed. Finally, the opportunities and challenges for DNA assembly-based stimuli-responsive systems are overviewed and discussed.

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Section: Dna Assembly‐based Stimuli‐responsive Dna Systemsmentioning

confidence: 99%

DNA Assembly‐Based Stimuli‐Responsive Systems

Fan

et al. 2021

Advanced Science

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“…These properties of nucleic acids provided a rich “tool box” of modules to develop the area of DNA nanotechnology . Unique two-dimensional (2D) and three-dimensional (3D) DNA structures, DNA switches and machines, nucleic acid based catalysts, stimuli-responsive nucleic acid/nanoparticle hybrid structures, and signal-triggered DNA materials were developed using these properties of DNA. Different applications of these systems were discussed in the field of sensors, controlled drug release and self-healing materials, cell imaging and therapeutic applications, logic gates and computing circuits, and more.…”

Section: Introductionmentioning

confidence: 99%

Nucleic Acid Based Constitutional Dynamic Networks: From Basic Principles to Applications

et al. 2020

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Inspired by nature where intracellular dynamic interactions between DNA, RNA and proteins processed within complex networks leading to programmed reaction patterns, extensive research efforts are directed to mimic these processes by chemical means, "Systems Chemistry". The present perspective introduces nucleic acids as functional modules to construct constitutional dynamic networks, CDNs, mimicking natural networks. The base sequences comprising nucleic acids provide a rich "tool box" to assemble signal-triggered reconfigurable CDNs revealing adaptive and hierarchically adaptive properties, intercommunication between CDNs, and feedback-driven reaction pathways similar to natural systems. Pathways for the evolution of CDNs and the formation of networks of enhanced complexities are discussed. Different applications of constitutional dynamic networks are introduced including programmed catalysis, CDN-guided optical and catalytic functions of nanoparticle aggregates, and CDN-dictated stiffness and self-healing functions of hydrogels. Future perspectives of the field in designing dissipative transient CDNs, CDNs-guided transcription/translation synthesis of selective proteins, and the challenging integration of CDNs into cell-like containments aiming to assemble "artificial cells" are addressed.

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“…The eliciting of sequence-specific nucleic acids (aptamers) recognizing low-molecular-weight ligands, macromolecules and even cells by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure [ 1 , 2 , 3 , 4 ] introduced revolutionary means to apply nucleic acids as functional materials for bioanalytical [ 5 , 6 ], catalytic [ 7 , 8 , 9 ] and biomedical [ 9 , 10 , 11 , 12 ] applications. The selective formation of aptamer-ligand complexes enabled the application of aptamers as versatile recognition elements for the development of sensor devices and sensing platforms, aptasensors [ 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

Vázquez‐González

Willner

2021

IJMS

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Sequence-specific nucleic acids exhibiting selective recognition properties towards low-molecular-weight substrates and macromolecules (aptamers) find growing interest as functional biopolymers for analysis, medical applications such as imaging, drug delivery and even therapeutic agents, nanotechnology, material science and more. The present perspective article introduces a glossary of examples for diverse applications of aptamers mainly originated from our laboratory. These include the introduction of aptamer-functionalized nanomaterials such as graphene oxide, Ag nanoclusters and semiconductor quantum dots as functional hybrid nanomaterials for optical sensing of target analytes. The use of aptamer-functionalized DNA tetrahedra nanostructures for multiplex analysis and aptamer-loaded metal-organic framework nanoparticles acting as sense-and-treat are introduced. Aptamer-functionalized nano and microcarriers are presented as stimuli-responsive hybrid drug carriers for controlled and targeted drug release, including aptamer-functionalized SiO2 nanoparticles, carbon dots, metal-organic frameworks and microcapsules. A further application of aptamers involves the conjugation of aptamers to catalytic units as a means to mimic enzyme functions “nucleoapzymes”. In addition, the formation and dissociation of aptamer-ligand complexes are applied to develop mechanical molecular devices and to switch nanostructures such as origami scaffolds. Finally, the article discusses future challenges in applying aptamers in material science, nanotechnology and catalysis.

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Catalytic Nucleic Acids for Bioanalysis

Cited by 20 publications

References 183 publications

DNA Assembly‐Based Stimuli‐Responsive Systems

DNA Assembly‐Based Stimuli‐Responsive Systems

Nucleic Acid Based Constitutional Dynamic Networks: From Basic Principles to Applications

Aptamer-Functionalized Hybrid Nanostructures for Sensing, Drug Delivery, Catalysis and Mechanical Applications

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