Catalytic Profile and Amylolytic Studies of Toluene-Tolerant Enterococcus faecalis str. nov. mercadA7

Meruvu, Haritha

doi:10.1007/s13369-016-2386-x

Cited by 2 publications

(2 citation statements)

References 35 publications

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“…Its chitinolytic activity is visualized by streaking over minimal salt (MS)-chitin agar plate containing 0.5% w/v colloidal chitin with MS medium [12], incubated and observed for 72 h at 30 °C. The strain showed a striking zone of clearance of nearly 0.62 cm after 24 h. It is maintained on yeast extract-malt extract agar slants and subcultured every 30 days [18]. Initial fermentation conditions for chitinase production are 24 h incubation at 30 °C, 0.5%v/w inoculum (10 9 CFU/ml), 30% v/w moisture and 8 pH using 5 g of wheat bran.…”

Section: Microorganism and Fermentation Studiesmentioning

confidence: 99%

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Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

Meruvu

2018

SN Appl. Sci.

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Solid phase fermentation of wheat bran coupled with powdered fish scales could yield chitinase using a novel Citrobacter strain. The fermentation conditions are optimized conventionally (84.14 U/gds) and statistically (94.3 U/gds). The partially purified chitinase had a specific activity of 53.41 U/mg with optimal activity at pH 8.5 and 50 °C, it was stable at 7.5-10 pH range for 24 h with more than 80% stability at 45-60 °C, and the Km value of chitinase with swollen chitin (substrate) is 8.11 mg/ml with a V max of 2.43 mmol h −1 ml −1 . The partially purified enzyme was halotolerant showing maximum activity and stability up to 10% of Nacl, also possessing potential anticoagulant activity. This is the first report to date elucidating the production of halotolerant chitinase from wheat bran supplemented with fish scales using Citrobacter freundii haritD11 and its application as an anticoagulant agent and notable tolerance to heavy metal ions.

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Section: Microorganism and Fermentation Studiesmentioning

confidence: 99%

“…Fractions with enzyme are pooled to measure enzyme activity and protein content. Chitinase activity and protein content were analyzed in supernatant and the pellet [16,18]…”

Section: Purification Studiesmentioning

confidence: 99%

Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

Meruvu

2018

SN Appl. Sci.

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Polyextremotolerant Amylase Produced from Novel Enterococcus with Potpourri of Applications

Meruvu

2019

Int J Pept Res Ther

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Catalytic Profile and Amylolytic Studies of Toluene-Tolerant Enterococcus faecalis str. nov. mercadA7

Cited by 2 publications

References 35 publications

Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

Polyextremotolerant Amylase Produced from Novel Enterococcus with Potpourri of Applications

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