Catalytic properties of thioredoxin immobilized on superparamagnetic nanoparticles

Netto, Caterina Gruenwaldt Cunha Marques; Nakamatsu, Eduardo Hiroshi; Netto, Luís Eduardo Soares; Novak, Miguel A.; Zuin, Andre; Nakamura, Marcelo; Araki, Koji; Toma, Henrique E.

doi:10.1016/j.jinorgbio.2011.02.006

Cited by 18 publications

(14 citation statements)

References 39 publications

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“…5 The modification of APTS endows magnetic iron oxide nanoparticles with excellent water dispersibility, colloidal stability, and positive charge. 19 Although many studies examining APTS-MNPs have been reported, 14,16,20,21 few report on APTS-MNPs in their in vivo experiments. Therefore, we used the traditional methods of the addition of polyamine hydrobromide and MTT assay to facilitate the incorporation of nanoparticles and measure cell proliferation, respectively.…”

Section: Discussionmentioning

confidence: 99%

In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

Li¹,

Li²,

Qin³

et al. 2013

IJN

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Background: Stem cell transplantation has been investigated for repairing damaged tissues in various injury models. Monitoring the safety and fate of transplanted cells using noninvasive methods is important to advance this technique into clinical applications. Methods: In this study, lower-limb ischemia models were generated in nude mice by femoral artery ligation. As negative-contrast agents, positively charged magnetic iron oxide nanoparticles (aminopropyltriethoxysilane-coated Fe 2 O 3 ) were investigated in terms of in vitro labeling efficiency, effects on human mesenchymal stromal cell (hMSC) proliferation, and in vivo magnetic resonance imaging (MRI) visualization. Ultimately, the mice were sacrificed for histological analysis three weeks after transplantation. Results: With efficient labeling, aminopropyltriethoxysilane-modified magnetic iron oxide nanoparticles (APTS-MNPs) did not significantly affect hMSC proliferation. In vivo, APTS-MNP-labeled hMSCs could be monitored by clinical 3 Tesla MRI for at least three weeks. Histological examination detected numerous migrated Prussian blue-positive cells, which was consistent with the magnetic resonance images. Some migrated Prussian blue-positive cells were positive for mature endothelial cell markers of von Willebrand factor and anti-human proliferating cell nuclear antigen. In the test groups, Prussian blue-positive nanoparticles, which could not be found in other organs, were detected in the spleen. Conclusion: APTS-MNPs could efficiently label hMSCs, and clinical 3 Tesla MRI could monitor the labeled stem cells in vivo, which may provide a new approach for the in vivo monitoring of implanted cells.

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Section: Discussionmentioning

confidence: 99%

In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

Li¹,

Li²,

Qin³

et al. 2013

IJN

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“…APTES on the surface of magnetic support, which provides a high density of surface functional groups for coupling [26]. Silane coatings enhance enzyme binding to the support and improve enzyme stability [27]. The addition of NHS to EDC is also known to decrease the hydrolysis of active ester (o-acylisourea) from EDC activating formation in aqueous solution, leading to increased efficiency of the EDC-mediated coupling reaction [28] together with the improvement of solubility and stability of active intermediate groups [29].…”

Section: Catalytic Activity Of Magnetic Nanoparticle Lipasesmentioning

confidence: 99%

Modification of magnetic nanoparticle lipase designs for biodiesel production from palm oil

Raita

Arnthong

Champreda

et al. 2015

Fuel Processing Technology

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“…Supermagnets of Nd2Fe14B displaying strong magnetic fields are quite available in the market, and are suitable for this purpose. However, one has to carefully consider the biomolecule linking procedure to the nanoparticle beforehand, since the coupling method can impact the enzyme catalytic activity and its immobilization efficiency (Rebelo et al, 2010;Netto et al, 2009Netto et al, , 2011Netto et al, , 2012Netto et al, , 2013Netto et al, , 2015. A large variety of methods has already been employed (Netto et al, 2013); however, they have led to very contrasting results.…”

Section: Introductionmentioning

confidence: 99%

Lipase immobilized on polydopamine-coated magnetite nanoparticles for biodiesel production from soybean oil

Andrade

Parussulo

Netto

et al. 2016

BRJ

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Catalytic properties of thioredoxin immobilized on superparamagnetic nanoparticles

Cited by 18 publications

References 39 publications

In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

Modification of magnetic nanoparticle lipase designs for biodiesel production from palm oil

Lipase immobilized on polydopamine-coated magnetite nanoparticles for biodiesel production from soybean oil

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