Catch and Release of DNA in Coacervate‐Dispersed Gels

Ohsugi, Asami; Furukawa, Hidemitsu; Kakugo, Akira; Osada, Yoshihito; Gong, Jian Ping

doi:10.1002/marc.200600234

Cited by 27 publications

(25 citation statements)

References 14 publications

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“…In general, heteroaggregation is defined as the aggregation of dissimilar particles, which may differ in their size, shape, charge, chemical composition, and other properties . Heteroaggregation has been widely used in nonfood science applications for a variety of reasons, such as controlling the rheological properties of ceramics, creating ion‐exchange columns, removing colloidal particles from aqueous solutions, and encapsulating and targeting biomolecules . Heteroaggregation of oppositely charged particles through electrostatic attraction is the most commonly used method for most applications, and therefore this method will be the focus of this review.…”

Section: Principles Of Heteroaggregationmentioning

confidence: 99%

Modulation of food texture using controlled heteroaggregation of lipid droplets: Principles and applications

Mao

McClements

2013

J of Applied Polymer Sci

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This article reviews the development and potential application of novel food-grade materials based on heteroaggregation of oppositely charged particles. These particles typically consist of a hydrophobic lipid core and an electrically charged emulsifier shell. Heteroaggregation leads to the formation of emulsion-based products that are highly viscous or gel-like at much lower droplet concentrations than in nonaggregated systems. Heteroaggregation may therefore be useful for developing food products with novel textural characteristics or for creating reduced-calorie versions of full fat foods. We give an overview of the principles of heteroaggregation, the relationship between emulsion microstructure and texture, and possible commercial applications of this method.

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Section: Principles Of Heteroaggregationmentioning

confidence: 99%

Modulation of food texture using controlled heteroaggregation of lipid droplets: Principles and applications

Mao

McClements

2013

J of Applied Polymer Sci

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“…Such association can take the form of layer-bylayer assembly of polymers on a charged substrate, 1,2 or it could involve charge complexation in solution. [3][4][5] Examples of emerging technologies that rely on these and related phenomena include wet adhesives for medical, construction, consumer, and military use, 6,7 advanced biosensors, drug and gene delivery vehicles, [8][9][10] and responsive and switchable surfaces. 11,12 When the association takes place in solution, depending on the strength of the polyelectrolyte and the solvent conditions, the resulting polyelectrolyte complex can either be a solid precipitate or a polyelectrolyte-rich liquid phase termed a "complex coacervate."…”

Section: Introductionmentioning

confidence: 99%

Investigation of the interfacial tension of complex coacervates using field-theoretic simulations

Riggleman

Kumar

Fredrickson

2012

The Journal of Chemical Physics

107

130

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Complex coacervation, a liquid-liquid phase separation that occurs when two oppositely charged polyelectrolytes are mixed in a solution, has the potential to be exploited for many emerging applications including wet adhesives and drug delivery vehicles. The ultra-low interfacial tension of coacervate systems against water is critical for such applications, and it would be advantageous if molecular models could be used to characterize how various system properties (e.g., salt concentration) affect the interfacial tension. In this article we use field-theoretic simulations to characterize the interfacial tension between a complex coacervate and its supernatant. After demonstrating that our model is free of ultraviolet divergences (calculated properties converge as the collocation grid is refined), we develop two methods for calculating the interfacial tension from field-theoretic simulations. One method relies on the mechanical interpretation of the interfacial tension as the interfacial pressure, and the second method estimates the change in free energy as the area between the two phases is changed. These are the first calculations of the interfacial tension from full fieldtheoretic simulation of which we are aware, and both the magnitude and scaling behaviors of our calculated interfacial tension agree with recent experiments. Complex coacervation, a liquid-liquid phase separation that occurs when two oppositely charged polyelectrolytes are mixed in a solution, has the potential to be exploited for many emerging applications including wet adhesives and drug delivery vehicles. The ultra-low interfacial tension of coacervate systems against water is critical for such applications, and it would be advantageous if molecular models could be used to characterize how various system properties (e.g., salt concentration) affect the interfacial tension. In this article we use field-theoretic simulations to characterize the interfacial tension between a complex coacervate and its supernatant. After demonstrating that our model is free of ultraviolet divergences (calculated properties converge as the collocation grid is refined), we develop two methods for calculating the interfacial tension from field-theoretic simulations. One method relies on the mechanical interpretation of the interfacial tension as the interfacial pressure, and the second method estimates the change in free energy as the area between the two phases is changed. These are the first calculations of the interfacial tension from full fieldtheoretic simulation of which we are aware, and both the magnitude and scaling behaviors of our calculated interfacial tension agree with recent experiments.

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“…The interfacial tension between these two phases is an important factor that is directly related to the interaction between the macroions. In all potential applications of coacervate phases, such as drug carriers, [2][3][4] fat replacers or meat analogues, 5,6 coatings and packaging materials, 7 knowledge of the interfacial tension is essential in making predictions about the prerequisites and practical applicability of such systems. In complex coacervate core micelles for example, 8,9 the interfacial tension is related to the driving force of micelle formation, and it can thus be used to predict the critical aggregation concentration.…”

Section: Introductionmentioning

confidence: 99%

Interfacial tension between a complex coacervate phase and its coexisting aqueous phase

et al. 2010

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Complex coacervation is the associative phase separation in a solution of positively and negatively charged macroions. Despite the widespread use of coacervation in e.g. micellar assemblies (complex coacervate core micelles), drug carriers and thin films, there is virtually no experimental data on the interfacial tension between such coacervate phases (polyelectrolyte complexes) and their coexisting aqueous phases or on the influence of salt thereon. In this paper we use colloidal probe AFM measurements of capillary adhesion forces to obtain the interfacial tension between a complex coacervate phase of two polyelectrolytes with high charge density and its coexisting aqueous phase. We find that the interfacial tension is of order 100 mN/m, decreases with increasing salt concentration and vanishes at the critical point. Interestingly, we find that the critical scaling exponent for the interfacial tension found in segregative demixing also applies here.

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Catch and Release of DNA in Coacervate‐Dispersed Gels

Cited by 27 publications

References 14 publications

Modulation of food texture using controlled heteroaggregation of lipid droplets: Principles and applications

Modulation of food texture using controlled heteroaggregation of lipid droplets: Principles and applications

Investigation of the interfacial tension of complex coacervates using field-theoretic simulations

Interfacial tension between a complex coacervate phase and its coexisting aqueous phase

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