Catching sight of lysosomes

Wells, William A.

doi:10.1083/jcb1682fta1

Cited by 3 publications

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Preparation of Single Cells for Imaging Mass Spectrometry

Berman¹,

Fortson²,

Kulp

2010

Methods in Molecular Biology

View full text Add to dashboard Cite

Characterizing chemical changes within single cells is important for determining fundamental mechanisms of biological processes that will lead to new biological insights and improved disease understanding. Imaging biological systems with mass spectrometry (MS) has gained popularity in recent years as a method for creating precise chemical maps of biological samples.In order to obtain high-quality mass spectral images that provide relevant molecular information about individual cells, samples must be prepared so that salts and other cell-culture components are removed from the cell surface and the cell contents are rendered accessible to the desorption beam. We have designed a cellular preparation protocol for imaging MS that preserves the cellular contents for investigation and removes the majority of the interfering species from the extracellular matrix. Using this method, we obtain excellent imaging results and reproducibility in three diverse cell types: MCF7 human breast cancer cells, Madin-Darby canine kidney (MDCK) cells, and NIH/3T3 mouse fibroblasts. This preparation technique allows routine imaging MS analysis of cultured cells, allowing for any number of experiments aimed at furthering scientific understanding of molecular processes within individual cells.

show abstract

Preparation of Single Cells for Imaging Mass Spectrometry

Berman¹,

Fortson²,

Kulp

2010

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

Preparation of single cells for imaging/profiling mass spectrometry

Berman

Fortson

Checchi

et al. 2008

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Characterizing chemical changes within individual cells is important for determining fundamental mechanisms of biological processes that will lead to new biological insights and improved disease understanding. Analyzing biological systems with imaging and profiling mass spectrometry (MS) has gained popularity in recent years as a method for creating chemical maps of biological samples. To obtain mass spectra that provide relevant molecular information about individual cells, samples must be prepared so that salts and other cell culture components are removed from the cell surface and that the cell contents are rendered accessible to the desorption beam. We have designed a cellular preparation protocol for imaging/profiling MS that removes the majority of the interfering species derived from the cellular growth medium, preserves the basic morphology of the cells, and allows chemical profiling of the diffusible elements of the cytosol. Using this method, we are able to reproducibly analyze cells from three diverse cell types: MCF7 human breast cancer cells, Madin-Darby canine kidney (MDCK) cells, and NIH/3T3 mouse fibroblasts. This preparation technique makes possible routine imaging/profiling MS analysis of individual cultured cells, allowing for understanding of molecular processes within individual cells.

show abstract

Overview of Advanced 3D Charge-trapping Flash Memory Devices

Lue

Hsieh

2010

MRS Proc.

View full text Add to dashboard Cite

Although conventional floating gate (FG) Flash memory has already gone into the sub-30 nm node, the technology challenges are formidable beyond 20nm. The fundamental challenges include FG interference, few-electron storage caused statistical fluctuation, poor short-channel effect, WL-WL breakdown, poor reliability, and edge effect sensitivity. Although charge-trapping (CT) devices have been proposed very early and studied for many years, these devices have not prevailed over FG Flash in the > 30nm node. However, beyond 20nm the advantage of CT devices may become more significant. Especially, due to the simpler structure and no need for charge storage isolation, CT is much more desirable than FG in 3D stackable Flash memory. Optimistically, 3D CT Flash memory may allow the Moore's law to continue for at least another decade. In this paper, we review the operation principles of CT devices and several variations such as MANOS and BE-SONOS. We will then discuss 3D memory architectures including the bit-cost scalable approach. Technology challenges and the poly-silicon thin film transistor (TFT) issues will be addressed in detail.

show abstract

Catching sight of lysosomes

Cited by 3 publications

References 10 publications

Preparation of Single Cells for Imaging Mass Spectrometry

Preparation of Single Cells for Imaging Mass Spectrometry

Preparation of single cells for imaging/profiling mass spectrometry

Overview of Advanced 3D Charge-trapping Flash Memory Devices

Contact Info

Product

Resources

About