Catechol grafted silica particles for enhanced adhesion to metal by coordinate bond

Ionic Diffusion‐Driven Ionovoltaic Transducer for Probing Ion‐Molecular Interactions at Solid–Liquid Interface

“…After the CA functionalization, the surface zeta‐potential was shifted from highly positive values (Figure S1 , Supporting Information; 74.6 ± 0.03 and 57.9 ± 0.1 mV at DI water and 0.1 m conditions, respectively) to slightly negative regions (Figure S1 , Supporting Information; −5.12 ± 0.20 and −1.99 ± 0.04 mV at DI water and 0.1 m conditions, respectively) due to an influence of hydroxyl group in CA. [ 31 ] To investigate the effect of the Fe 3+ ‐CA complexation in SAM to IIT, the generated voltage variation upon exposures of FeCl 3 solution (from 10 −7 to 10 −2 m) was monitored as shown in Figure 2b,c . As the exposed solution concentration increased, the magnitude of the generated voltages in the Fe 3+ adsorbed device (Fe‐IIT) was elevated up to ≈9.6 mV (10 −3 m ) with presenting similar time‐dependent profiles.…”

High-Throughput Signal-On Photoelectrochemical Immunoassay of Lysozyme Based on Hole-Trapping Triggered by Disintegrating Bioconjugates of Dopamine-Grafted Silica Nanospheres

“…The sequential functionalizations on the surface of silica were evidenced by FT-IR spectra (Figure B). The representative absorption peaks for pristine SiO 2 NS are observed at 1104 cm –1 (asymmetric stretching vibration of Si–O–Si), 955 cm –1 (asymmetric bending and stretching vibration of Si–OH), and 800 cm –1 (stretching vibration of Si–C), respectively (curve a). With the amine-modification, the characteristic adsorption peaks of the N–H bending vibration were observed at 1550–1640 cm –1 , confirming the successful introduction of amine groups onto the SiO 2 NPs surface (curve b).…”

“…First, SiO 2 NSs as well as surface-functionalized SiO 2 NSs with amine groups (SiO 2 NSs-NH 2 ) were prepared by a modified Stöber method (see Supporting Information for details). ,, The obtained SiO 2 NSs-NH 2 were dispersed in a mixture of ethanol and DMF ( v : v = 1:1), collected by centrifugation, and then redispersed in DMF (50 mL). Following that, the dispersion of SiO 2 NSs-NH 2 was added slowly into 50 mL DMF containing succinic anhydride (0.5 M) under stirring.…”

“…To prepare the bioconjugate of DA@SiO 2 NSs-labeled detection antibody, SiO 2 NSs-COOH was first activated with EDC and NHS to generate NHS ester-terminated SiO 2 NSs (SiO 2 NSs-NHS). , Subsequently, anti-Lyz detection antibody (designated as Ab 2 , 0.5 mg mL –1 , 20 μL) and DA (0.1 M, 40 μL) were added into the above solution (detailed procedures were listed in Supporting Information). The resulting bioconjugate (designed as Ab 2 -DA@SiO 2 NSs) was stored in a refrigerator at 4 °C.…”

“…To further simplify the operation, the elaborate design via integrating an electron donor with the PEC sensing system is highly desirable. Owing to the outstanding chemical stability and biocompatibility, silica nanospheres (SiO 2 NSs), in particular, SiO 2 NSs-based bioconjugates, have been widely used for various biomedical applications. , Dopamine (DA), serving as a binder, is bioadhesive to inorganic and organic surfaces. , Through a 1-(3-(dimethylamino)­propyl)-3-ethylcarbodiimidehydrochloride (EDC)/ N -hydroxysuccinimide (NHS) coupling reaction, catechol groups can be modified on the surface of silica. , Inspired by this, we construct dopamine-grafted silica nanospheres (DA@SiO 2 NSs) as molecular printboards for positioning biomolecules, i.e., used as labeling the detection antibodies. Accompanying the specific antigen–antibody reaction, a sandwich immunoassay can be fabricated with an electron donor (DA) embedded.…”