Catechol Oxidase, Phosphatase-Like Activity, DNA/BSA Binding Studies of RuII Complexes of S-Allyldithiocarbazate: Synthesis and Spectral Studies

Abstract

New ruthenium(II) complexes with formulae [RuCl(CO)(PPh 3 ) 2 L 1 ] and [RuCl(CO)(PPh 3 ) 2 L 2 ] (L 1 = S-allyl-4-methoxy benzylidene hydrazine carbodithioate, L 2 = S-allyl-1-naphthylidene hydrazine carbodithioate) have been synthesized and characterized by elemental analysis, Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), UV-Vis, electrospray ionization (ESI)-mass spectral studies. Both the Schiff base ligands are coordinated to ruthenium through azomethine nitrogen (C=N) and thiolato sulfur donor atoms in the thiolate form. On the basis of spectral data obtained, an octahedral structure has been assigned to all the new complexes, satisfied by coordination of donor atoms N and S. The stability of complexes in solution was determined by molar conductivity measurements. The interaction of the two complexes with calf thymus (CT) DNA was investigated by fluorescence spectroscopic and viscometric methods. Bovine serum albumin (BSA) protein was examined by fluorescence spectroscopic methods. These techniques indicate that the two metal complexes bind to DNA via intercalation mode and BSA bind with static interaction. Catecholase and phosphatase like activities promoted by these two complexes under physiological conditions have been studied. In vitro anticancer activities have been demonstrated by 3,4,5-dimethylthiazolyl-2-2,5-diphenyltetrazolium bromide (MTT) assay, acridine orange/ethidium bromide (AO/EB) and diamidino-2-phenylindole (DAPI) staining against HeLa cancer cell line.

Catecholase-Like Activity and Theoretical Study in Solid State of a New Ru(III)-Schiff Base Complex

“…The catalytic conversion of 3,5-DTBC to 3,5-DTBQ (Scheme 2) has already been investigated by a number of scientist as a model catalytic reaction by using a variety of mono-, [45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60] di-, 31,[51][52][53][54][55][56][57][58][59][60][61][62][63] tri/tetra-, [64][65][66] and poly-nuclear metal complexes. 67 3,5-DTBC is the most widely used model substrate in catalytic oxidation process due to its low redox potential value that favours the oxidation to 3,5-DTBQ, which shows absorbance maximum at 400 nm in DMSO.…”