Catechol Siderophores Control Tungsten Uptake and Toxicity in the Nitrogen-Fixing Bacterium <i>Azotobacter vinelandii</i>

Wichard, Thomas; Bellenger, Jean‐Philippe; Loison, Aurélie; Kraepiel, Anne M. L.

doi:10.1021/es702651f

Cited by 49 publications

(54 citation statements)

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“…W is in many cases interchanged with Mo in enzymes and might be biologically very relevant in those three bacterial strains by providing enzymatic function. Sometimes the enzymatic function is preserved [57], but in most cases, W inhibits cell growth [58]. To lower inhibition, bacteria can excrete catechol siderophores that could form complexes with W, thereby diminishing its availability for microorganisms [58].…”

Section: Discussionmentioning

confidence: 99%

Pattern of Elemental Release During the Granite Dissolution Can Be Changed by Aerobic Heterotrophic Bacterial Strains Isolated from Damma Glacier (Central Alps) Deglaciated Granite Sand

et al. 2011

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Colonisation and weathering of freshly deglaciated granite are key processes in initial soil formation and development. We have obtained 438 isolates from granite sand covering glacial toe, 284 isolates at 22°C and 154 at 4°C incubation temperatures, respectively, to obtain cultures for the investigation of their weathering capabilities under laboratory conditions. The isolation of bacteria from granite sand was performed on rich-, intermediate- and low-nutrient-content solid media. Isolates were identified by 16S rRNA gene sequencing. According to the genera-associated weathering capabilities described in the literature and according to their abundance in our culture collection, we selected eight strains to analyse their effects on the weathering dynamics of granite sand during the batch culture experiment. Analysis of culturable bacteria showed higher species richness among isolates from 22°C than from 4°C incubations. In the R2A and 1/100 Ravan media, we observed the highest species richness of isolates obtained at 22°C and 4°C incubation temperatures, respectively. The obtained 16S rRNA sequences revealed the presence of alpha-, beta- and gamma-proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. The most numerous group of isolates was distantly related to Collimonas representatives, and according to the sequences of the 16S rRNA genes, they can form a new genus. Isolates from this group had the capability of causing increased dissolution rates for Fe, W, Ni and Rb. In general, at each sampling during the 30-day experiment, every strain showed a unique weathering profile resulting from differential rates of the dissolution and the precipitation of different minerals in the batch culture. Consequently, the presence of different strains, their growth stage and changes in proportions of strains in the bacterial community can affect further soil development and the successive colonisation by plants.

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Section: Discussionmentioning

confidence: 99%

Pattern of Elemental Release During the Granite Dissolution Can Be Changed by Aerobic Heterotrophic Bacterial Strains Isolated from Damma Glacier (Central Alps) Deglaciated Granite Sand

et al. 2011

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“…The uptake system(s) for the V-azotochelin and V-protochelin complexes apparently is specific, since neither the V complexes with DFB and aerobactin nor the W complexes with azotochelin and protochelin are taken up (36). It also is regulated by the concentration of V (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…But even when Fe is not limiting, protochelin and azotochelin accumulate at micromolar concentrations in the growth medium and bind vanadate in strong 1:1 complexes (log K app V-Az ϭ 8.8 at pH 6.6, where K app is the apparent complexation constant and V-Az represents the V-azotochelin complex) (2; Bellenger et al, unpublished). Metals bound to organic ligands usually are not available for uptake, as shown for the protochelin and azotochelin complexes of tungsten (W) in A. vinelandii (36). But A. vinelandii cells grown at low [V] can take up the V-azotochelin and V-protochelin complexes, indicating the existence of a specific uptake system for these complexes (Bellenger et al, unpublished).…”

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Vanadium Requirements and Uptake Kinetics in the Dinitrogen-Fixing Bacterium Azotobacter vinelandii

Bellenger

Wichard

Kraepiel

2008

Appl Environ Microbiol

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Vanadium is a cofactor in the alternative V-nitrogenase that is expressed by some N 2 -fixing bacteria when Mo is not available. We investigated the V requirements, the kinetics of V uptake, and the production of catechol compounds across a range of concentrations of vanadium in diazotrophic cultures of the soil bacterium Azotobacter vinelandii. In strain CA11.70, a mutant that expresses only the V-nitrogenase, V concentrations in the medium between 10 ؊8 and 10 ؊6 M sustain maximum growth rates; they are limiting below this range and toxic above. A. vinelandii excretes in its growth medium micromolar concentrations of the catechol siderophores azotochelin and protochelin, which bind the vanadate oxoanion. The production of catechols increases when V concentrations become toxic. Short-term uptake experiments with the radioactive isotope 49 V show that bacteria take up the V-catechol complexes through a regulated transport system(s), which shuts down at high V concentrations. The modulation of the excretion of catechols and of the uptake of the V-catechol complexes allows A. vinelandii to precisely manage its V homeostasis over a range of V concentrations, from limiting to toxic.

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“…The N 2 -fixing bacterium, Azotobacter vinelandii, produces catechol siderophores in response to the presence of tungsten. These siderophores can distinguish targets (i.e., iron and molybdenum) from tungsten that would be toxic to the bacterium due to deactivation of nitrogenase (Wichard et al, 2008). It is possible that some members of the soil microbial community isolated during this study may also produce these catechol siderophores and could therefore discriminate between tungsten and desired elements.…”

Section: Discussionmentioning

confidence: 96%

Impact of Sodium Tungstate and Tungsten Alloys on the Growth of Selected Microorganisms with Environmental Significance

Mumy¹,

Doyle²

2010

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The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB Control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE (DD MM YY) SPONSOR/MONITOR'S ACRONYM(S)NHRC/EHEL SPONSOR/MONITOR'S REPORT NUMBER(s) DISTRIBUTION/AVAILABILITY STATEMENTApproved for public release; distribution is unlimited. SUPPLEMENTARY NOTES ABSTRACTX A brief (200 words max) factual summary of the most significant information.Tungsten is a transition metal with unique properties that permit its use in a range of applications, including household products and small caliber ammunition. Increased use has restored interest in tungsten and tungsten-based products in determining not only their impacts upon human health, but also on the environment. Given the dependence on microbes for environmental processes, it is appropriate to evaluate tungsten effect on microorganisms. The goal of this study was to investigate the impact of sodium tungstate (Na 2 WO 4 ) and tungsten alloys on the growth of selected environmental microorganisms that play roles in metal reduction, biogeochemical cycling and biodegradation. A soil community was also evaluated for its tolerance to Na 2 WO 4 . Shewanella, a strong metal reducer, displayed the most robust ability to grow in the highest concentrations of Na 2 WO 4 evaluated, whereas Pseudomonas displayed lower tolerances. Interestingly, bacteria cultivated from the environment displayed only minor delays and reduction in growth relative to pure cultures, suggesting that such a microbial consortium is better suited to cope with Na 2 WO 4 exposure. Tungsten alloys also had profound effects on bacterial growth, however, these were dependent on the metals and nutrients present, suggesting the effect may be exacerbated in certain environmental settings where nutrients may be limited. SUBJECT TERMS Disclaimer:This work was supported by Defense Health Programs (DHP) reimbursable Work Unit #60769.The views expressed in this article are those of the authors and do not reflect the official policy or position of the Department of the Navy, Department of Defense, or the U.S. Government.This article is approved for public release, distribution unlimited. Acknowledgements:The authors thank Dr. Scott M. Lohrke for support in the initial stages of this study. In additio...

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Catechol Siderophores Control Tungsten Uptake and Toxicity in the Nitrogen-Fixing Bacterium Azotobacter vinelandii

Cited by 49 publications

References 28 publications

Pattern of Elemental Release During the Granite Dissolution Can Be Changed by Aerobic Heterotrophic Bacterial Strains Isolated from Damma Glacier (Central Alps) Deglaciated Granite Sand

Pattern of Elemental Release During the Granite Dissolution Can Be Changed by Aerobic Heterotrophic Bacterial Strains Isolated from Damma Glacier (Central Alps) Deglaciated Granite Sand

Vanadium Requirements and Uptake Kinetics in the Dinitrogen-Fixing Bacterium Azotobacter vinelandii

Impact of Sodium Tungstate and Tungsten Alloys on the Growth of Selected Microorganisms with Environmental Significance

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