Catecholamine Levels in the Guinea Pig Ovary, Myometrium and Costo-uterine Muscle During the Estrous Cycle and in the Ovary Remaining After Unilateral Ovariectomy

Farrar, John A.; Handberg, Gwynneth M.; Hartley, Margaret L.; Pennefather, Jocelyn N.

doi:10.1095/biolreprod22.3.473

Cited by 23 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After one complete oestrous cycle had been monitored, as described by Farrar et al [10], guinea-pigs were used on day 6-10 (dioestrus) of the oestrous cycle (group 1) or treated with steroids as described previously [4]. Thus one group (group 2) received thrice weekly injections of oestradiol cypionate (20 ~tg/kg s.c. in 0.05-0.1 ml peanut oil) for 14 days before use and another group (group 3) received identical treatment with oestradiol cypionate, and in addition were given progesterone (3 mg/animal s.c. in 0.1 ml peanut oil) daily for 4-5 days before use.…”

Section: Methodsmentioning

confidence: 99%

The actions of histamine on the separated layers of the guinea-pig uterus: the influence of ovarian steroids

Mammen

Pennefather

1988

Agents and Actions

View full text Add to dashboard Cite

The effects of histamine have been studied on separated circular and longitudinal myometrial layers from the guinea-pig. Virgin guinea-pigs in dioestrus were (1) untreated, (2) treated for 14 days thrice weekly with oestradiol cypionate 20 micrograms/kg s.c., or (3) treated as in (2) but in addition with progesterone 3 mg/animal s.c. daily for 4 days before use. Preparations were field-stimulated (60 V 2 ms 30 Hz for 5 s every 100 s) to elicit regular contractions. Histamine was equipotent in producing enhancement of evoked contractions of circular myometrium from animals in each treatment group (pD2 = 5.03, 5.05 and 4.95 in groups 1, 2 & 3 respectively), and enhanced contractility in all but two longitudinal myometrial preparations. Treatment with oestradiol alone, and with oestradiol and progesterone following oestradiol priming enhanced the magnitude of maximal response and produced a small decrease in the potency of histamine on the longitudinal myometrium. pA2 estimates for mepyramine as an antagonist were similar (8.6) in preparations of longitudinal myometrium from each group of animals. Metiamide (1-10 mumol/l) did not antagonise the effects of histamine in this layer. These results, taken together, indicate that ovarian steroids act selectively on the longitudinal myometrial layer to amplify the action of histamine, but do not alter the histamine receptor subtype which in this layer is exclusively H1.

show abstract

Section: Methodsmentioning

confidence: 99%

The actions of histamine on the separated layers of the guinea-pig uterus: the influence of ovarian steroids

Mammen

Pennefather

1988

Agents and Actions

View full text Add to dashboard Cite

show abstract

“…Ovarian nerves may participate in the development of compensatory hypertrophy after unilateral ovariectomy (Burden & Lawrence, 1977;Gerendai, Marchetti, Mauger, Roxas & Scapagnini, 1978;Gerendai & Halasz, 1978;Gerendai, 1980). In the guinea-pig, the onset of the hypertrophy has been temporally associated with a decrease in the ovarian content of norepinephrine (Farrar, Handberg, Hartley & Pennefather, 1980) and it was suggested that ovarian adrenergic nerves may normally exert a tonic inhibitory influence on the selection of follicles for maturation and/or ovulation.…”

Section: Introductionmentioning

confidence: 99%

Effect of ovarian sympathectomy on follicular development during compensatory ovarian hypertrophy in the guinea-pig

Curry¹,

Lawrence²,

Burden³

1984

Reproduction

View full text Add to dashboard Cite

Selective ovarian sympathectomy was achieved by injecting 6-hydroxydopamine into a surgically closed periovarian bursa on Day 3 of the oestrous cycle (Day 1 = day of oestrus). Control ovaries had the periovarian bursa surgically closed and were injected with solvent. On Day 15 of the cycle, serum was collected for progesterone determination and ovaries were processed for morphometric analysis of follicles. In both control and sympathectomized ovaries remaining after unilateral ovariectomy on Day 3, there was an increase in ovarian weight and an increase in healthy preovulatory follicles (greater than 700 microns diam.). Sympathectomy of the ovary remaining after unilateral ovariectomy increased healthy follicles 510-700 microns diameter but decreased the total number of follicles per ovary. In animals bearing both ovaries, unilateral sympathectomy did not affect any of the characteristics measured. Serum progesterone concentrations were unaffected by any of the treatments. These results indicate that adrenergic nerves play a role in follicular dynamics in ovaries undergoing compensatory hypertrophy but are not necessary for compensatory increases in weight and number of preovulatory follicles.

show abstract

“…It has been suggested that smooth cells in the tubo-uterine junction may provide a uterine pacemaker (Alvarez & Caldeyro-Barcia, 1953;Owman, 1981). Noradrenaline levels within this region, unlike those in the body of the uterus do not change during the oestrous cycle (Farrar et al, 1980), following the administration of oestrogen and progesterone (Handberg, unpublished observations), during pregnancy (Thorbert, 1978) or in the post-partum period (Thorbert, 1978;Hartley, Story, Farrar & Pennefather, 1983). Thus it is possible that neuronal and hormonal sympathetic activation may initiate contractions via effects upon a-adrenoceptors located on these pacemaker cells, irrespective of reproductive status.…”

Section: Discussionmentioning

confidence: 99%

“…Experiments in this laboratory (Farrar et al, 1980; have established that although the fluctuations in ovarian hormone levels occurring during the oestrous cycle of the guinea-pig can modify the levels of catecholamines within the longitudinal myometrium, those within the costo-uterine muscle are unaffected. This difference may be due, in part, to the differences in the innervation of the two tissues, since, unlike the costo-uterine muscle, the longitudinal myometrial layer receives a proportion of its sympathetic innervation via short adrenergic nerves which are susceptible to the influence of ovarian steroids (see review by Marshall, 1981 (Story, Hartley & Pennefather, 1980) and to the 8th International Congress of Pharmacology (Hartley, Story & Pennefather, 1981 (Farrar et al, 1980). Mean cycle length of these animals was 15.9 ± 0.3 days (number of cycles = 62, number of animals= 51).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of ovarian steroids upon responses mediated by adrenoceptors in separated layers of the myometrium and in the costo‐uterine muscle of the guinea‐pig

Hartley

Pennefather

Story

1983

British J Pharmacology

Self Cite

View full text Add to dashboard Cite

1This study describes the effects of ovarian steroid hormones upon the responses to adrenoceptor agonists of isolated myometrium, separated into its longitudinal and circular layers, and of costo-uterine muscle from guinea-pigs. The preparations were field-stimulated at 100s intervals, and the adrenoceptor agonists phenylephrine and isoprenaline produced enhancement or inhibition of the evoked contractions. 2 Isoprenaline produced propranolol-sensitive inhibitory effects in longitudinal and circular myometrium and costo-uterine muscle preparations from animals from all experimental groups: i.e. from nonsteroid-treated animals (ovariectomized and intact); intact animals treated with either oestrogen or progesterone alone; ovariectomized animals treated with oestrogen; ovariectomized and intact animals treated with progesterone following oestrogen priming; and from animals 1-4 days post-partum. Longitudinal myometrial preparations from progesterone-treated oestrogenprimed and from post-partum animals were most sensitive to this agonist. 3 Phenylephrine produced phentolamine-sensitive excitatory effects in circular myometrial and costo-uterine muscle preparations from animals from all the experimental groups. In contrast, propranolol-sensitive inhibitory responses to phenylephrine occurred in longitudinal myometrial preparations taken from animals treated with progesterone following oestrogen priming, and from post-partum animals. Longitudinal myometrium from animals from the remaining experimental groups exhibited phentolamine-sensitive excitatory responses to phenylephrine. 4 The basis for the selective effect upon the longitudinal myometrium of exposure to progesterone following a period of oestrogen priming, is discussed. The results described are consistent with the possibility that in the longitudinal layer of guinea-pig uterus exposed to progesterone following oestrogen priming there is an increase in the proportion of P-adrenoceptors in this layer. This increase may reduce the likelihood of contractions arising via direct stimulation of oa-adrenoceptors in this layer in response to sympathetic activation during pregnancy.

show abstract

Catecholamine Levels in the Guinea Pig Ovary, Myometrium and Costo-uterine Muscle During the Estrous Cycle and in the Ovary Remaining After Unilateral Ovariectomy

Cited by 23 publications

References 0 publications

The actions of histamine on the separated layers of the guinea-pig uterus: the influence of ovarian steroids

The actions of histamine on the separated layers of the guinea-pig uterus: the influence of ovarian steroids

Effect of ovarian sympathectomy on follicular development during compensatory ovarian hypertrophy in the guinea-pig

Effects of ovarian steroids upon responses mediated by adrenoceptors in separated layers of the myometrium and in the costo‐uterine muscle of the guinea‐pig

Contact Info

Product

Resources

About