Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X<sub>4</sub>

Du, Errong; Wang, Anhui; Fan, Rongping; Rong, Li-Lou; Yang, Runan; Xing, Juping; Shi, Xuguo; Qiao, Bao; Yu, Ruoyang; Xu, Changshui

doi:10.1080/10799893.2021.1878536

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…The culture of the primary spinal cord microglia was carried out as previously published . The neonatal 2–3 d SD rats were taken, and the spinal cords were carefully obtained.…”

Section: Methodsmentioning

confidence: 99%

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Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Yang

Shi

et al. 2023

ACS Chem. Neurosci.

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Ferroptosis is an inflammatory programmed cell death process that is dependent on iron deposition and lipid peroxidation. The P2X7 receptor not only is involved in the pain process but also is closely related to the onset of depression. Gallic acid (3,4,5-trihydroxybenzoic acid), which is naturally found in a variety of plants, exhibits anti-inflammatory, antioxidant, and analgesic effects. This study established a rat model with the comorbidity of chronic constrictive injury (CCI) plus chronic unpredictable mild stress (CUMS) to explore the role and mechanism of gallic acid in the treatment of pain and depression comorbidity. Our experimental results showed that pain and depression-like behaviors were more obvious in the chronic constriction injury (CCI) plus chronic unpredictable mild stimulation (CUMS) group than they were in the sham operation group, and the P2X7-reactive oxygen species (ROS) signaling pathway was activated. The tissue iron concentration was increased, and mitochondrial damage was observed in the CCI plus CUMS group. These results were alleviated with gallic acid treatment. Therefore, we speculate that gallic acid inhibits the ferroptosis of the spinal microglia by regulating the P2X7-ROS signaling pathway and relieves the behavioral changes in rats with comorbid pain and depression.

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“…The culture of the primary spinal cord microglia was carried out as previously published . The neonatal 2–3 d SD rats were taken, and the spinal cords were carefully obtained.…”

Section: Methodsmentioning

confidence: 99%

“…The culture of the primary spinal cord microglia was carried out as previously published. 25 The neonatal 2−3 d SD rats were taken, and the spinal cords were carefully obtained. After digestion, centrifugation, and resuspension, the mixed glial cell suspension was obtained.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Yang

Shi

et al. 2023

ACS Chem. Neurosci.

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“…The final concentration of naringin (Aladdin, CAS.10236-47-2) was 80 μM (Chen et al, 2017). The final concentration of ATP (Solarbio, CAS.987-65-5) was 0.1 mM, and phosphate-buffered saline (PBS) was used as the solvent (Du et al, 2022). When the cell confluence reached about 80%, naringin was added for 12 h, and ATP was added for 3 h for subsequent experiments.…”

Section: Primary Culturementioning

confidence: 99%

Naringin inhibits P2X4 receptor expression on satellite glial cells in the neonatal rat dorsal root ganglion

Wang,

Chen,

Xing

et al. 2023

J Appl Biomed

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Naringin inhibits inflammation and oxidative stress, the P2 purinoreceptor X4 receptor (P2X4R) is associated with glial cell activation and inflammation, the purpose of this study is to investigate the effects of naringin on P2X4 receptor expression on satellite glial cells (SGCs) and its possible mechanisms. ATP promoted the SGC activation and upregulated P2X4R expression; naringin inhibited SGC activation, decreased expression of P2X4R, P38 MAPK/ERK, and NF-κB, and reduced levels of Ca 2+ , TNF-α, and IL-1β in SGCs in an ATPcontaining environment. These findings suggest that naringin attenuates the ATP-induced SGC activation and reduces P2X4R expression via the Ca 2+ -P38 MAPK/ERK-NF-κB pathway.

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Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X₄

Cited by 2 publications

References 26 publications

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Naringin inhibits P2X4 receptor expression on satellite glial cells in the neonatal rat dorsal root ganglion

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Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X4

Cited by 2 publications

References 26 publications

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats

Naringin inhibits P2X4 receptor expression on satellite glial cells in the neonatal rat dorsal root ganglion

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Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X₄