Central venous catheters are useful for a variety of clinical situations being used in delivery of fluids and drugs, monitoring, and allowing for large volume of blood to be aspirated. Despite their usefulness, placement of venous access catheters is accompanied by the potential of causing catheter-related bloodstream infection (CRBSI). Attention to preparation of the catheter insertion site, selection of appropriate catheter material, proper hand hygiene, and optimal dressing material and methods play a large role in reduction of CRBSI. A central venous catheter maintenance protocol outlining dressing evaluation and changes, cleaning of the insertion site, and conditions for catheter removal will further improve the chances of an infection-free patient. Veterinary nurses play a vital role in the placement and maintenance of central venous catheters and influence a positive patient outcome.