Cation Accumulation by Muscle Tissue: The Displacement of Potassium by Rubidium and Cesium in the Living Animal12

Relman, Arnold S.; Lambie, Anne T.; Burrows, Belton A.; Roy, Arlene M.

doi:10.1172/jci103522

Cited by 82 publications

(45 citation statements)

References 16 publications

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“…The same tendency was observed for the sum of the Rb+ and K+ contents of Rb+-loaded cells, although the sum always exceeded the K+ content of the control. This indicates that cells accumulate Rb+ in preference to K+ as reported for other tissues (30,34). Cells may accumulate Rb+ rather than K+ for the following reasons : the higher affinity of Rb+ for negative charges in cells, an integrated effect of imperceptible cation selectivity undetected in the short-term experiments, or enhanced cation selectivity in the transport system, which might occur during long-term incubation with Rb+.…”

Section: Discussionsupporting

confidence: 71%

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

Miyamoto

Sakai

Ikehara

et al. 1978

Cell Struct. Funct.

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ABSTRACT. The usefulness of Rb+ was investigated as an analog of K+ in membrane transport and in supporting the growth of HeLa cells. On replacement of extracellular K+ by Rb+, intracellular Rb+ content increased linearly at 15 min. K+ content declined in contrast with Rb+, but Na+ content was unaffected. In Rb+-preloaded cells, Rb+ and K+ were exchanged after replacement of extracellular Rb+ with K. A kinetic analysis of intracellular Rb+ content showed that most K+ was exchangeable with Rb+, but a small part of K+ was not. Both Rb+ and K+ influxes were equally inhibited by ouabain or furosemide. Rb+ efflux was repressed by furosemide, whereas K+ efflux was not influenced. When 90 % of K+ was replaced by Rb+ in serum-free chemically defined medium, cell growth and protein synthesis were inhibited. After total replacement of extracellular K+ by Rb+, cell growth ceased and cellular protein content decreased, while a small part of intracellular K+ remained unexchanged. HeLa cells seemed to accumulate Rb+ in preference to K+.

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Section: Discussionsupporting

confidence: 71%

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

Miyamoto

Sakai

Ikehara

et al. 1978

Cell Struct. Funct.

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“…133 Cs þ and 87 Rb þ , the other K þ analog, are both much more sensitive to the NMR experiment than is 39 K, whose NMR receptivity is roughly 100 times less than that of the two analogs. 5 133 Cs þ accumulates in the intracellular space 6,7 primarily by the action of the Na þ -K þ pump, 2,3,[6][7][8][9][10][11][12][13] and has a transmembrane permeability of 0.1-0.2 of that of K þ . Rubidium has a transmembrane permeability that is roughly equivalent to that of K þ , [14][15][16] perhaps because the ionic radius of Rb þ (148 pm) is more similar to K þ (133 pm) than is that of Cs þ (167 pm).…”

Section: Introductionmentioning

confidence: 99%

Biomedical applications of133Cs NMR

Goodman

Ackerman

2005

NMR Biomed.

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133 Cs NMR is a valuable tool for non-invasively probing biological systems. As a congener of potassium, it accumulates in the intracellular space, primarily through the action of the Na þ -K þ pump (ATPase). In addition, it is possible to resolve the MR signal of 133 Cs in different tissue compartments on the basis of chemical shift or MR relaxation properties. This compartmental resolution applies not only to the intra-and extracellular spaces, but to subcellular compartments as well. In this review, we discuss the studies defining the ion transport, chemical shift and relaxation characteristics of 133 Cs in living systems. We also review the application of 133 Cs NMR to evaluation of ion transport across membranes and the kinetic/ chemical environment of the intracellular space in systems ranging from red blood cells to rat brain.

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“…Ru bidium administered exogenously is reportedly distributed in various tissues including the brain (7-9) and is excreted very slowly (10,11). Repeated administrations of rubidium in certain doses, therefore, result in exhibiting toxic effects such as a decrease in body weight (8,12) and tetanic spasms or convulsion (12)(13)(14)(15). The safety margin in the dosage of rubidium is narrow therefore if a toxic dose is given, the effects observed would be non-specific rather than specific.…”

Section: Discussionmentioning

confidence: 99%

Effects of Rubidium on Behavioral Responses to Methamphetamine and Tetrabenazine

Furukawa

Tokuda

1976

Japanese Journal of Pharmacology

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Abstract-Different groups of mice were injected subcutaneoLisly every other day with rubidium chloride at three doses (0.41(50), 1.23(150) and 3.69(450) meq,'kg (mg/kg)) or with saline as a control for a period of 2-3 weeks. Rubidium administered acutely did not affect spontaneous locomotor activities, while it tended to increase the activities when administered repeatedly though the increase was not statistically significant. The methamphetamine-induced hyperlocomotor activities were potentiated in the rubidium groups as compared with those in the saline group, this effect of rubidium being in creased with prolongation of repeated administrations. Monotonic decreases in ambu lation after tetrabenazine were not significantly affected in the rubidium-treated animals though the decreases were sometimes preceded by slight increases and recovery from the decrement tended to be more rapid. After tetrabenazine in the rubidium-treated groups, incidences of catalepsy were increased and jumping behavior and Straub tail responses occurred in a few cases. The results suggest that rubidium potentiates the excitatory action of methamphetamine on spontaneous locomotor activities, as con trasted with inhibitory influence of lithium.Rubidium and lithium, alkaline metals, have been proposed to have valuable therapeutic and prophylactic effects in manic-depressive psychosis; rubidium is principally effective in depressive states (1, 2) while lithium is effective mainly for manic states. In studies on ani mals, the behavioral activation caused by morphine is potentiated by rubidium in mice whereas it is antagonized by lithium (3). Thus rubidium and lithium presumably have opposite effects in all species.Many studies have also been done on the neurochemical mechanisms underlying the affective disorders. The catecholamine hypothesis of affective disorders is that some, if not all, depressions are associated with an absolute or relative decrease in catecholamines, available at central adrenergic receptors sites, while elation, conversely, may be associated with an excess of such amines (4, 5). Associated with this hypothesis has been the proposal by one of the present authors (T.F.) that lithium modifies behavioral responses to me thamphetamine and tetrabenazine and that these drugs act on the metabolism of endogenous brain catecholamines, inhibiting in particular the methamphetamine-induced excitation (6).The present investigation was an attempt to study the effects of rubidium on behavioral responses induced by methamphetamine and tetrabenazine. MATERIALS AND METHODSHealthy ddY male albino mice obtained from the Kuroda Animal Laboratory (Kuma

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Cation Accumulation by Muscle Tissue: The Displacement of Potassium by Rubidium and Cesium in the Living Animal12

Cited by 82 publications

References 16 publications

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

Effect of Rb+ Substituted for K+ on HeLa Cells: Cellular Content and Membrane Transport of Monovalent Cations, and Cell Growth

Biomedical applications of133Cs NMR

Effects of Rubidium on Behavioral Responses to Methamphetamine and Tetrabenazine

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