Cation permeability induced by spermine and polybrene in rat liver mitochondria

Huunan-Seppälä, A

doi:10.1007/bf01648914

Cited by 10 publications

(2 citation statements)

References 31 publications

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“…According to the Maxwell-Boltsmann distribution, the concentration of an ion in the unstirred layer adjacent to a membrane depends on the surface potential of the membrane and indeed it has been shown that the surface charge of artificial and biological membranes can influence their passive and energy-linked permeability to ions [33,19] and metabolites [29,40]. Kinetically, this change in local concentration of the substrate would alter the apparent /d~ of transport but not the Vigax [22].…”

Section: Discussionmentioning

confidence: 97%

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Fry

Goldman

1982

J. Membrain Biol.

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Interaction of positively (phosphatidylcholine/stearylamine 5:1) or negatively (phosphatidylcholine/stearic acid 5:1) charged liposomes with Ehrlich ascites tumor cells for 1-5 min increases or decreases, respectively, the bidirectional fluxes of the folic acid analog, methotrexate. These effects on influx and efflux appear to be symmetrical since the liposomes do not change the intracellular level of methotrexate at the steady state. Influx kinetics show that these alterations result from an increase or decrease in the Vmax with no change in the Kinm. These effects appear to be specific for the methotrexate-tetrahydrofolate carrier system since the transport of other compounds which utilize this carrier, aminopterin, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate, is affected similarly to methotrexate, whereas, the transport of folic acid, a compound similar in structure and charge but not significantly transported by this carrier is unaffected by liposomes. Once cells are exposed to charged liposomes, the effects on methotrexate transport cannot be reversed by washing the cells free of the extracellular liposomes. If, however, cells are exposed to liposomes of one charge, washed and then exposed to liposomes of the opposite charge, methotrexate influx is reversed to control rates. The effects of charged liposomes on methotrexate influx were not abolished by treating the cells with neuraminidase, metabolic inhibitors or lowering the temperature to 4 degrees C. Studies on the uptake of [14C] liposomes show that these effects are not proportional to the total amount of lipid associated with the cell but result from an initial rapid liposome-cell association that is not dependent on temperature or energy metabolism nor related to cell surface charge.

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Section: Discussionmentioning

confidence: 97%

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Fry

Goldman

1982

J. Membrain Biol.

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“…Because anionic lipids impart a negative charge to the surfaces of lipid bilayers, it has been assumed that one of the specific functions of anionic lipids is the regulation of membrane lipid surface charge density (see Christiansson et al, 1985;Clementz and Christiansson, 1986; Clementz et al, 1987;and references cited therein). Indeed, lipid bilayer surface charge density can have major effects on the passive permeability of biological membranes to ions and charged metabolites and on the activity of a number of membrane-bound enzymes and transport proteins (see Huunan-Seppalla, 1971;Rowohl-Quisthoudt, 1975, 1976;McLaughlin et al, 1970;Theuvenet et al, 1976a,b;Wojtczaj and Nalecz, 1979; and references cited therein). Others have shown that membrane lipid surface charge density affects the growth, transbilayer membrane potential, and adenylate energy charge of Acholeplasma laidlawii cells, and that lipid surface charge density appears to be biosynthetically regulated in this organism (Christiansson et al, 1985;Clementz and Christiansson, 1986;Clementz et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Calorimetric and Spectroscopic Studies of the Thermotropic Phase Behavior of the n-Saturated 1,2-Diacylphosphatidylglycerols

Yuanpeng¹,

Lewis²,

McElhaney³

1997

Biophysical Journal

166

123

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The polymorphic phase behavior of a homologous series of n-saturated 1,2-diacyl phosphatidylglycerols (PGs) was studied by differential scanning calorimetry and Fourier transform infrared and 31P-nuclear magnetic resonance spectroscopy. When dispersed in aqueous media under physiologically relevant conditions, these compounds exhibit two thermotropic phase transitions that are structurally equivalent to the well-characterized pretransitons and gel/liquid-crystalline phase transitions exhibited by bilayers of the corresponding 1,2-diacyl phosphatidylcholines. Furthermore, when incubated at low temperatures, their gel phases spontaneously transform into one or more solid-like phases that appear to be highly ordered, quasicrystalline bilayers that are probably partially dehydrated. The quasicrystalline structures, which form upon short-term, low-temperature annealing of these lipids, are meta-stable with respect to more stable structures, to which they eventually transform upon prolonged low-temperature incubation. The rates of formation of the quasicrystalline phases of the PGs generally tend to decrease as hydrocarbon chain length increases, and PGs whose hydrocarbon chains contain an odd number of carbon atoms tend to be slower than those of neighboring even-numbered homologs. The calorimetric data also indicate that the quasicrystalline phases of these compounds become progressively less stable relative to both their gel and liquid-crystalline phases as the length of the hydrocarbon chain increases and that they decompose either to the liquid-crystalline phase (short- and medium-chain compounds) or to the normal gel phase (long-chain compounds) upon heating. The spectroscopic data indicate that although there is odd-even alternation in the structures of the quasicrystalline phases formed upon short-term low-temperature incubation of these compounds, the structural features of the stable quasicrystalline phases eventually formed are all similar. Furthermore, the degree of hydration and the nature of hydrogen bonding interactions in the headgroup and interfacial regions of these PG bilayers differ significantly from that observed in all other phospholipid bilayers studied so far. We suggest that many of the properties of PG bilayers can be rationalized by postulating that the glycerol moiety of the polar headgroup is directly involved in shielding the negative charges at the surface of the bilayer by means of hydration-like hydrogen bonding interactions with the phosphate moiety.

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Bidirectional Transport of Spermine Across the Inner Membrane of Liver Mitochondria

Toninello

Siliprandi

Castagnini

et al. 1988

Progress in Polyamine Research

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Cation permeability induced by spermine and polybrene in rat liver mitochondria

Cited by 10 publications

References 31 publications

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Calorimetric and Spectroscopic Studies of the Thermotropic Phase Behavior of the n-Saturated 1,2-Diacylphosphatidylglycerols

Bidirectional Transport of Spermine Across the Inner Membrane of Liver Mitochondria

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