Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Xu, Xuemei; Fang, Shiqi; Zhuang, Yuan; Wu, Shanshan; Pan, Qingling; Li, Longjie; Wang, Xiaohui; Sun, Xun; Liu, Bi‐Feng; Wu, Yuzhou

doi:10.3390/ma12060949

Cited by 23 publications

(19 citation statements)

References 37 publications

(45 reference statements)

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“…4d) [47]. A similar effect was observed when using a chemically modified protein-cationic human serum albumin (HSA)-that is more biocompatible and easily available [48]. These investigations clearly demonstrated that decoration of DNA nanostructures with cationic polymers could be an efficient way to improve their physiological stability and modulate their in vitro and in vivo distribution.…”

Section: Polymer Modified Dna Nanomaterialsmentioning

confidence: 59%

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

Winterwerber

et al. 2020

Top Curr Chem (Z)

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DNA nanotechnology, based on sequence-specific DNA recognition, could allow programmed self-assembly of sophisticated nanostructures with molecular precision. Extension of this technique to the preparation of broader types of nanomaterials would significantly improve nanofabrication technique to lower nanometer scale and even achieve single molecule operation. Using such exquisite DNA nanostructures as templates, chemical synthesis of polymer and inorganic nanomaterials could also be programmed with unprecedented accuracy and flexibility. This review summarizes recent advances in the synthesis and assembly of polymer and inorganic nanomaterials using DNA nanostructures as templates, and discusses the current challenges and future outlook of DNA templated nanotechnology.

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Section: Polymer Modified Dna Nanomaterialsmentioning

confidence: 59%

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

Winterwerber

et al. 2020

Top Curr Chem (Z)

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“… 161–163 Xu et al coated DNA origami with cationic human serum albumin protein via electrostatic interaction. 163 The positive encapsulation was able to not only increase the transfection into HeLa cells by threefold, but also improve the stability of DNA origami under physiological conditions and from Deoxyribonuclease I (DNase I), a specific endonuclease commonly used to breakdown chromatin. A similar strategy was applied to coat dendrons of DNA origami with bovine serum albumin through cysteine-maleimide bond.…”

Section: Programmed Delivery Of Dna Nanostructuresmentioning

confidence: 99%

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

et al. 2020

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DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.

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“…These hybrid systems can be extended to assemble multiple proteins, construct enzyme cascades and fabricate biosensors or biochips [ 82 , 83 ]. The generation of nucleic acid-protein conjugates is a promising and versatile tool to enhance gene delivery, modify the solubility, biological activity and stability of therapeutic cargos or the nanostructure [ 84 , 85 , 86 , 87 , 88 ]. As an unrivaled class of macromolecular drugs with high specificity, proteins are biodegradable, easily metabolized and amenable to surface modifications [ 89 ].…”

Section: Nucleic Acid Hybrid Nanocarriersmentioning

confidence: 99%

Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers

Obuobi

Škalko‐Basnet

2020

Pharmaceutics

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Conventional antibiotic therapy is often challenged by poor drug penetration/accumulation at infection sites and poses a significant burden to public health. Effective strategies to enhance the therapeutic efficacy of our existing arsenal include the use of nanoparticulate delivery platforms to improve drug targeting and minimize adverse effects. However, these nanocarriers are often challenged by poor loading efficiency, rapid release and inefficient targeting. Nucleic acid hybrid nanocarriers are nucleic acid nanosystems complexed or functionalized with organic or inorganic materials. Despite their immense potential in antimicrobial therapy, they are seldom utilized against pathogenic bacteria. With the emergence of antimicrobial resistance and the associated complex interplay of factors involved in antibiotic resistance, nucleic acid hybrids represent a unique opportunity to deliver antimicrobials against resistant pathogens and to target specific genes that control virulence or resistance. This review provides an unbiased overview on fabricating strategies for nucleic acid hybrids and addresses the challenges of pristine oligonucleotide nanocarriers. We report recent applications to enhance pathogen targeting, binding and control drug release. As multifunctional next-generational antimicrobials, the challenges and prospect of these nanocarriers are included.

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Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Cited by 23 publications

References 37 publications

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

DNA-Programmed Chemical Synthesis of Polymers and Inorganic Nanomaterials

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers

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