Cationic and hydrolysable branched polymers by RAFT for complexation and controlled release of dsRNA

Abstract

The complexation and sustained release of dsRNA from highly branched polymers prepared via RAFT polymerisation and copolymerisation of the monomers DMAEA, DMAPA, and DMAEMA, is reported.

Hyperbranched poly(ethylenimine-co-oxazoline) by thiol–yne chemistry for non-viral gene delivery: investigating the role of polymer architecture

“…Commonly used synthetic vectors include cationic polymers, liposomes, and covalent polymer conjugates. [10][11][12] Cationic polymers such as poly-L-lysine (PLL), polyethylenimine (PEI), poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA), and polyamidoamine dendrimers (PAMAM) are promising systems for the non-viral trafficking of genetic material. Despite their popularity, high cytotoxicity associated with their polycationic nature, which tends to cause disruption of cellular membranes, remains a major limitation.…”

Alcohol mediated degenerate chain transfer controlled cationic polymerisation of para-alkoxystyrene

“…The advancement of macromolecular synthesis has enabled the creation of complex architectures and functional materials. [1][2][3][4][5][6][7] Although controlled radical polymerisation methods have dominated this area in general, 8,9,14 materials generated by cationic polymerisation offer unique properties that are not readily accessible by radical chemistry. 10,11 In contrast, controlled cationic polymerisation has gained less attention, due to the synthetic challenge in controlling the highly reactive propagating cationic species that often leads to more side reactions.…”

Competitive binding and molecular crowding regulate the cytoplasmic interactome of non-viral polymeric gene delivery vectors

“…We focused on PDMAEMA and PMETAC brush-functionalised nanoparticles as cationic model systems. PDMAEMA-based vectors have been widely studied and applied for gene delivery 16,17 . The high density of polymer brushes was found to bind RNA oligonucleotides particularly stably 18 , yet display low cytotoxicity and be amenable to molecular design, including labelling of corresponding nanomaterials 19 .…”