Cationic carbosilane dendrimers–lipid membrane interactions

Wrobel, Dominika; Kłys, Arkadiusz; Йонов, Максим; Vitovič, Pavol; Waczulikowa, Iveta; Hianik, Tibor; Gomez-Ramirez, Rafael; Mata, F. Javier de la; Klajnert, Barbara; Bryszewska, Maria

doi:10.1016/j.chemphyslip.2012.01.008

Cited by 32 publications

(24 citation statements)

References 45 publications

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“…3. The hydrodynamic diameters of the DMPC or DMPC/DPPG liposomes in controls were 111 ± 1.7 and 104 ± 1.6 nm, respectively, in a good agreement with the diameter of polycarbonate filter pores used for liposome preparation as well as with our previous studies [17]. With increased concentration of peptides, the size of both types of LUVs was practically unchanged (Fig.…”

Section: Zeta Potential and Size Of Liposome/dendriplex Systemssupporting

confidence: 84%

“…It has been suggested that dendrimer core can also be involved in these interactions. The mechanisms of CBD-membrane interactions are, however, not fully understood yet [17]. Further, we studied also interaction of CBD modified by siRNA with unilamellar vesicles composed of DMPC or a mixture of DMPC and DPPG as well as DPPG alone.…”

Section: The Morphology Of Liposome-dendriplex Complexes Studied By Temmentioning

confidence: 97%

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Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Йонов

Ciepluch

Garaiova

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Section: Zeta Potential and Size Of Liposome/dendriplex Systemssupporting

confidence: 84%

Section: The Morphology Of Liposome-dendriplex Complexes Studied By Temmentioning

confidence: 97%

Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Йонов

Ciepluch

Garaiova

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Some dendrimers are able to remove lipids and create nanoholes in the structure of the lipid bilayer [39][40][41]. It has been shown that dendrimers change the properties of the lipid bilayer not only on the surface but also in the hydrophobic region [28,29,42,43]. Several authors have also focused their attention on the role of functional groups and the size of molecules in the investigated interactions [26,44].…”

Section: Discussionmentioning

confidence: 98%

“…After preparation, the suspension of liposomes was incubated in a water bath at 37°C for 10 min. For fluorescence experiments, the final lipid concentration was 100 μM, and the phospholipid/fluorescent probe molar ratio was 500:1 [28,29].…”

Section: Liposome Preparationmentioning

confidence: 99%

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Wrobel

Appelhans

Signorelli

et al. 2015

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The influence of maltose-modified poly(propylene imine) (PPI) dendrimers on dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) (3%) liposomes was studied. Fourth generation (G4) PPI dendrimers with primary amino surface groups were partially (open shell glycodendrimers - OS) or completely (dense shell glycodendrimers - DS) modified with maltose residues. As a model membrane, two types of 100nm diameter liposomes were used to observe differences in the interactions between neutral DMPC and negatively charged DMPC/DMPG bilayers. Interactions were studied using fluorescence spectroscopy to evaluate the membrane fluidity of both the hydrophobic and hydrophilic parts of the lipid bilayer and using differential scanning calorimetry to investigate thermodynamic parameter changes. Pulsed-filed gradient NMR experiments were carried out to evaluate common diffusion coefficient of DMPG and DS PPI in D2O when using below critical micelle concentration of DMPG. Both OS and DS PPI G4 dendrimers show interactions with liposomes. Neutral DS dendrimers exhibit stronger changes in membrane fluidity compared to OS dendrimers. The bilayer structure seems more rigid in the case of anionic DMPC/DMPG liposomes in comparison to pure and neutral DMPC liposomes. Generally, interactions of dendrimers with anionic DMPC/DMPG and neutral DMPC liposomes were at the same level. Higher concentrations of positively charged OS dendrimers induced the aggregation process with negatively charged liposomes. For all types of experiments, the presence of NaCl decreased the strength of the interactions between glycodendrimers and liposomes. Based on NMR diffusion experiments we suggest that apart from electrostatic interactions for OS PPI hydrogen bonds play a major role in maltose-modified PPI dendrimer interactions with anionic and neutral model membranes where a contact surface is needed for undergoing multiple H-bond interactions between maltose shell of glycodendrimers and surface membrane of liposome.

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“…To disrupt the packing order of the lipid chains, a greater amount of energy is required and thus the T m increases. 51,57,58 We have observed no major alterations in the thermal stability of DPPC vesicles, since the T m of fully hydrated DPPC MLVs detected at 41°C did not change neither with the encapsulated nor with the incubated SPIONs. We have observed some alterations in the DPPC peak shapes in the interaction of encapsulated SPIONs with DPPC.…”

Section: Phase Transition Of Liposomal Membranementioning

confidence: 90%

Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes

Santhosh

Drašler

Drobne

et al. 2015

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) with multifunctional properties have shown great promise in theranostics. The aim of our work was to compare the effects of SPIONs on the fluidity and phase transition of the liposomal membranes prepared with zwitterionic phosphatidylcholine lipids. In order to study if the surface modification of SPIONs has any influence on these membrane properties, we have used four types of differently functionalized SPIONs, such as: plain SPIONs (primary size was shown to bê11 nm), silica-coated SPIONs, SPIONs coated with silica and functionalized with positively charged amino groups or negatively charged carboxyl groups (the primary size of all the surface-modified SPIONs was ~20 nm). Small unilamellar vesicles prepared with 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine lipids and multilamellar vesicles prepared with 1,2-dipalmitoyl- sn -glycero-3-phosphocholine lipids were encapsulated or incubated with the plain and surface-modified SPIONs to determine the fluidity and phase transition temperature of the bilayer lipids, respectively. Fluorescent anisotropy and differential scanning calorimetric measurements of the liposomes that were either encapsulated or incubated with the suspension of SPIONs did not show a significant difference in the lipid ordering and fluidity; though the encapsulated SPIONs showed a slightly increased effect on the fluidity of the model membranes in comparison with the incubated SPIONs. This indicates the low potential of the SPIONs to interact with the nontargeted cell membranes, which is a desirable factor for in vivo applications.

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Cationic carbosilane dendrimers–lipid membrane interactions

Cited by 32 publications

References 45 publications

Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Interaction study between maltose-modified PPI dendrimers and lipidic model membranes

Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes

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