Cationic lipid bilayer coated gold nanoparticles-mediated transfection of mammalian cells

15 years on siRNA delivery: Beyond the State-of-the-Art on inorganic nanoparticles for RNAi therapeutics

“…Copyright 2015, Nature Publishing Group. [29,[44][45][46]. However, almost all nanoconjugates using siRNA have exclusively been tested in cell cultures and targeting only reporter genes (i.e.…”

“…Encapsulation is the least common approach, as it can be used with only a few types of inorganic nanoparticles, such as calcium phosphate nanoparticles [44,114,134], which can encapsulate the siRNA when added in the synthesis step. Afterwards, these nanoparticles induce the endosomal escape by disruption of the endosome membrane and upon degradation release the siRNA directly in the cytosol (Figure 13) [134].…”

Cationic Lipid-Coated Gold Nanoparticles as Efficient and Non-Cytotoxic Intracellular siRNA Delivery Vehicles

“…Particularly in drug delivery, AuNPs have emerged as an attractive candidate for the creation of efficient nucleic acid delivery systems, mostly due to their excellent biocompatibility, versatility in synthesis, high surface-area-to-volume ratio, and facile surface functionalization through intracellularly displaceable gold-thiol linkages (1,2). Until recently, various types of AuNPs have been widely explored for intracellular delivery of nucleic acid drugs, such as plasmid DNA, antisense oligonucleotide, and small interfering RNA (siRNA) (2)(3)(4)(5)(6)(7)(8)(9)(10). Furthermore, AuNPs functionalized with bio-recognizable ligands have been developed for specific cell-targeting and delivery applications (11,12).…”

One-Pot Synthesis of Cationic Gold Nanoparticles by Differential Reduction

“…Thus, the direct displacement of the negative citrate by positively charged molecules causes multiple electrostatic bridging, leading to irreversible agglomeration of the conjugates [29]. Alternatively, most direct synthetic approaches involves the reduction of an aqueous solution of Au 3+ precursor in the presence of cationic ligands [26,[30][31][32], or following minor modifications of the well-known Brust-Schiffrin two-phase methodology [10,33], which is generally limited to Au NPs of very small sizes (from 1.5 to 6 nm).…”