Cationic Liposomes in Different Structural Levels for Gene Delivery

Zhao, Yinan; Zhi, Defu; Zhang, Shubiao

doi:10.5772/17504

Cited by 4 publications

(4 citation statements)

References 131 publications

(142 reference statements)

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“…In our previous work, we synthesized a series of cationic lipids containing a hydroxyl moiety on the quaternary ammonium for liposome-mediated gene delivery, and some of them showed relatively higher transfection efficiency than the commercial transfection agents, Lipofectamine 2000 (Figure ). It was suggested that the headgroup hydration could be decreased by the incorporation of a hydroxyalkyl chain capable of hydrogen bonding to neighboring headgroups, while it improved the compaction of DNA by several mechanisms: for example, DNA could form hydrogen bonds with the lipid, and the hydroxyl group could enhance the membrane hydration …”

Section: Strategies To Improve Binding Affinity Charge Density and Tr...mentioning

confidence: 99%

“…It was suggested that the headgroup hydration could be decreased by the incorporation of a hydroxyalkyl chain capable of hydrogen bonding to neighboring headgroups, while it improved the compaction of DNA by several mechanisms: for example, DNA could form hydrogen bonds with the lipid, and the hydroxyl group could enhance the membrane hydration. 47 Accordingly, a number of cationic lipids with quaternary ammonium headgroups containing one or more hydroxyl moieties have been synthesized and showed higher transfection efficiency. 48−54 Banerjee et al 50 synthesized a series of nontoxic and nonglycerol-based cationic transfection lipids having one or more hydroxyalkyl chains on the quaternary ammonium headgroup.…”

Section: Charge Density and Transfection Efficiency On Ammonium And A...mentioning

confidence: 99%

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The Headgroup Evolution of Cationic Lipids for Gene Delivery

et al. 2013

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Cationic lipids are one of the most widely used nonviral vectors for gene delivery and are especially attractive because they can be easily synthesized and extensively characterized. Additionally, they can best facilitate the elucidation of structure-activity relationships by modifying each of their constituent domains. The polar hydrophilic headgroups enable the condensation of nucleic acids by electrostatic interactions with the negatively charged phosphate groups of the genes, and further govern transfection efficiency. The headgroups of cationic lipids play a crucial role for gene delivery; they can be quaternary ammoniums, amines, aminoacids or peptides, guanidiniums, heterocyclic headgroups, and some unusual headgroups. This review summarizes recent research results concerning the nature (such as the structure and shape of cationic headgroup) and density (such as the number and the spacing of cationic headgroup) of head functional groups for improving the design of efficient cationic lipids to overcome the critical barriers of in vitro and in vivo transfection.

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Section: Strategies To Improve Binding Affinity Charge Density and Tr...mentioning

confidence: 99%

Section: Charge Density and Transfection Efficiency On Ammonium And A...mentioning

confidence: 99%

The Headgroup Evolution of Cationic Lipids for Gene Delivery

et al. 2013

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“…We synthesized carbamate-linked cationic lipids for liposome-mediated gene delivery, which proved to have good gene transfection properties in different cell lines. As they are chemically stable and biodegradable, many cationic lipids of this kind have been synthesized (Liu, 2005a; Liu, 2005b; Liu, 2008; Zhao, 2011). To maintain the balance between serum stability and transfection efficiency, Chan et al (2012a, 2012b) have designed and synthesized a hydrolysable acid-labile PEG-lipid (HPEG-lipid, PEG MW 2000).…”

Section: Ph-responsive Cationic Lipidsmentioning

confidence: 99%

Lipid-based vectors for siRNA delivery

Zhang¹,

Zhi²,

Huang³

2012

Journal of Drug Targeting

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siRNA therapeutics has developed rapidly and already there are clinical trials ongoing or planned; however, the delivery of siRNA into cells, tissues or organs remains to be a major obstacle. Lipid-based vectors hold the most promising position among non-viral vectors, as they have a similar structure to cell or organelle membranes. But when used in the form of liposomes, these vectors have shown some problems. Therefore, either the nature of lipids themselves or forms used should be improved. As a novel class of lipid like materials, lipidoids have the advantages of easy synthesis and the ability for delivering siRNA to obtain excellent silencing activity. However, the toxicities of lipidoids have not been thoroughly studied. pH responsive lipids have also gained great attention recently, though some of the amine-based lipids are not novel in terms of chemical structures. More complex self-assembly structures, such as LPD (LPH) and LCP, may provide a good solution to siRNA delivery. They have demonstrated controlled particle morphology and size and siRNA delivery activity for both in vitro and in vivo.

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“…Since the nioplexes at the niosome/siRNA weight ratio of 15 had negative or neutral surface charges (Figure S5), it is possible that the siRNA dominance attached to the nioplex surfaces and did not incorporate into the lipid vesicles, which leads to siRNA degradation [73]. In addition, the large size of the 6as1(1.5) nioplexes at the niosome/siRNA weight ratio of 80 inhibits the effect of serum on transfection efficiency, facilitates cell membrane contact, and internalizes large intracellular vesicles, which were easily disrupted and released the siRNA from the nioplexes [74][75][76].…”

Section: Discussionmentioning

confidence: 99%

Dual-Targeted Therapy in HER2-Overexpressing Breast Cancer with Trastuzumab and Novel Cholesterol-Based Nioplexes Silencing Mcl-1

Pengnam,

Opanasopit,

Rojanarata

et al. 2023

Pharmaceutics

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The challenge in HER2-overexpressing breast cancer therapy lies in creating an effective target therapy to overcome treatment resistance. Monoclonal antibodies and target gene silencing by siRNA are two potential strategies that have been widely developed for treating HER2-positive breast cancer. The siRNA delivery system is a crucial factor that influences siRNA therapy’s success. In this study, lipid-based nanoparticles (cationic niosomes) composed of different cholesterol-based cationic lipids were formulated and characterized for delivering siRNA into HER2-overexpressing breast cancer cells. Niosomes containing a trimethylammonium headgroup showed the highest siRNA delivery efficiency with low toxicity. The myeloid cell leukemia-1 (Mcl-1) siRNA nioplex treatment significantly decreased mRNA expression and breast cancer cell growth. Dual-targeted therapy, consisting of treatment with an Mcl-1 siRNA nioplex and trastuzumab (TZ) solution, noticeably promoted cell-growth inhibition and apoptosis. The synergistic effect of dual therapy was also demonstrated by computer modeling software (CompuSyn version 1.0). These findings suggest that the developed cationic niosomes were effective nanocarriers for siRNA delivery in breast cancer cells. Furthermore, the Mcl-1 nioplex/TZ dual treatment establishes a synergistic outcome that may have the potential to treat HER2-overexpressing breast cancer.

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Cationic Liposomes in Different Structural Levels for Gene Delivery

Cited by 4 publications

References 131 publications

The Headgroup Evolution of Cationic Lipids for Gene Delivery

The Headgroup Evolution of Cationic Lipids for Gene Delivery

Lipid-based vectors for siRNA delivery

Dual-Targeted Therapy in HER2-Overexpressing Breast Cancer with Trastuzumab and Novel Cholesterol-Based Nioplexes Silencing Mcl-1

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