Cationic molecular probes based on aggregation-induced emission for fluorescent sensing and super-resolution imaging of insulin fibrosis

Mei, Li‐Jun; Cheng, Fan; Xu, Chu-Ran; Qi, Yu; Li, Chong; Wang, Yalong; Zhu, Ming‐Qiang

doi:10.1016/j.cej.2022.139027

Cited by 12 publications

(4 citation statements)

References 44 publications

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“…127,128 Table 1 lists the maximum absorption and emission, as well as the average number of photons detected per single event, for most AIE probes introduced in this paper. 30,129–140 AIE dyes (AIEgens) generally demonstrate larger Stokes shifts, commonly reaching 100–200 nm. Different AIE probes showed significant variations in the number of photons detected per single event.…”

Section: The Applications Of Aie In Super-resolution Imagingmentioning

confidence: 99%

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Recent advances in super-resolution optical imaging based on aggregation-induced emission

Zhu,

Mei,

Tian

et al. 2024

Chem. Soc. Rev.

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Section: The Applications Of Aie In Super-resolution Imagingmentioning

confidence: 99%

“…In 2022, Zhu et al 136 demonstrated the use of cationic AIE probes in combination with reversible dynamic binding through electrostatic interactions with negatively charged insulin fibrils for super-resolution imaging (Fig. 15).…”

Section: The Applications Of Aie In Super-resolution Imagingmentioning

confidence: 99%

Recent advances in super-resolution optical imaging based on aggregation-induced emission

Zhu,

Mei,

Tian

et al. 2024

Chem. Soc. Rev.

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“…Mei et al prepared probes 45 – 47 to study amyloid fibrosis [ 145 ]. As the main protein model, the authors chose insulin that possessed the determined structure and relatively low molecular weight.…”

Section: Fluorescent Probes Based On Aggregation-induced Emission (Aie)mentioning

confidence: 99%

Fluorescent Probes as a Tool in Diagnostic and Drug Delivery Systems

et al. 2023

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Over the last few years, the development of fluorescent probes has received considerable attention. Fluorescence signaling allows noninvasive and harmless real-time imaging with great spectral resolution in living objects, which is extremely useful for modern biomedical applications. This review presents the basic photophysical principles and strategies for the rational design of fluorescent probes as visualization agents in medical diagnosis and drug delivery systems. Common photophysical phenomena, such as Intramolecular Charge Transfer (ICT), Twisted Intramolecular Charge Transfer (TICT), Photoinduced Electron Transfer (PET), Excited-State Intramolecular Proton Transfer (ESIPT), Fluorescent Resonance Energy Transfer (FRET), and Aggregation-Induced Emission (AIE), are described as platforms for fluorescence sensing and imaging in vivo and in vitro. The presented examples are focused on the visualization of pH, biologically important cations and anions, reactive oxygen species (ROS), viscosity, biomolecules, and enzymes that find application for diagnostic purposes. The general strategies regarding fluorescence probes as molecular logic devices and fluorescence–drug conjugates for theranostic and drug delivery systems are discussed. This work could be of help for researchers working in the field of fluorescence sensing compounds, molecular logic gates, and drug delivery.

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“…A fluorescent sensor having a hybrid structure based on fluorene and Congo red has been proposed for the detection of abnormal beta-like conformations in lysozyme, insulin, and beta-2-microglobulin, and has shown changes in both absorption spectra and fluorescence intensity in the presence of all these proteins [ 34 ]. Polyfluorene probes carrying cationic groups are capable of interacting with the negatively charged residues of proteins through ionic bonds while emitting fluorescence as a result of the aggregation process (aggregation-induced emission) [ 35 ]. In addition, conjugated polymers based on fluorene and other fluorophores capable of fluorescence resonance energy transfer (FRET) are very useful as colorimetric and fluorimetric sensors for the point-of-care detection of serum albumin [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Fluorimetric Detection of Insulin Misfolding by Probes Derived from Functionalized Fluorene Frameworks

Sarabia-Vallejo,

Molina,

Martínez-Orts

et al. 2024

Molecules

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A group of functionalized fluorene derivatives that are structurally similar to the cellular prion protein ligand N,N′-(methylenedi-4,1-phenylene)bis [2-(1-pyrrolidinyl)acetamide] (GN8) have been synthesized. These compounds show remarkable native fluorescence due to the fluorene ring. The substituents introduced at positions 2 and 7 of the fluorene moiety are sufficiently flexible to accommodate the beta-conformational folding that develops in amyloidogenic proteins. Changes in the native fluorescence of these fluorene derivatives provide evidence of transformations in the amyloidogenic aggregation processes of insulin. The increase observed in the fluorescence intensity of the sensors in the presence of native insulin or amyloid aggregates suggest their potential use as fluorescence probes for detecting abnormal conformations; therefore, the compounds can be proposed for use as “turn-on” fluorescence sensors. Protein–sensor dissociation constants are in the 5–10 μM range and an intermolecular charge transfer process between the protein and the sensors can be successfully exploited for the sensitive detection of abnormal insulin conformations. The values obtained for the Stern–Volmer quenching constant for compound 4 as a consequence of the sensor–protein interaction are comparable to those obtained for the reference compound GN8. Fluorene derivatives showed good performance in scavenging reactive oxygen species (ROS), and they show antioxidant capacity according to the FRAP and DPPH assays.

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Cationic molecular probes based on aggregation-induced emission for fluorescent sensing and super-resolution imaging of insulin fibrosis

Cited by 12 publications

References 44 publications

Recent advances in super-resolution optical imaging based on aggregation-induced emission

Recent advances in super-resolution optical imaging based on aggregation-induced emission

Fluorescent Probes as a Tool in Diagnostic and Drug Delivery Systems

Fluorimetric Detection of Insulin Misfolding by Probes Derived from Functionalized Fluorene Frameworks

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