Cationic peptide of the male reproductive tract, HE2α, displays antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis

Liao, Mingmin; Ruddock, Patrick S.; Rizvi, Aleza; Hall, Susan H.; French, Frank S.; Dillon, Jo-Anne R.

doi:10.1093/jac/dki350

Cited by 21 publications

(16 citation statements)

References 10 publications

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“…Moreover, coagulase-negative staphylococci are well established due to nosocomial bacteremia and indwelling medical device-associated infection, showing increased multidrug resistance (1,14). Thus, the identification of novel agents effective in inhibiting these strains has gained renewed urgency (7). In addition, there is renewed interest in plants with antimicrobial properties as a consequence of current problems associated with the use of antibiotics (4,9).…”

mentioning

confidence: 99%

In Vitro Activities of Panduratin A against Clinical Staphylococcus Strains

Rukayadi

Lee

Han

et al. 2009

Antimicrob Agents Chemother

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mentioning

confidence: 99%

In Vitro Activities of Panduratin A against Clinical Staphylococcus Strains

Rukayadi

Lee

Han

et al. 2009

Antimicrob Agents Chemother

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“…It has been reported that in men the penile urethra is the entry for various STI-causing pathogens such as Neisseria gonorrhoeae and Chlamydia trachomatis , and urethritis is the most common clinical syndrome [5]. Infection originating from retrograde ascent of pathogens via the ejaculatory ducts, vas deferens, or the blood vessels supplying the epididymis is a common cause of acute epididymitis.…”

Section: Introductionmentioning

confidence: 99%

Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal CellsIn Vitro

Reddy

Sukanya

Patgaonkar

et al. 2012

International Journal of Peptides

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Antimicrobial peptides (AMP's) protect epithelial surfaces including epididymis against pathogens and play a key role in orchestrating various defensive responses. Recently, we have identified one such AMP, rabbit epididymal hemoglobin-β subuit (REHbβP) from the epididymal fluid of rabbit, Oryctologus cuniculus. The demonstration of a protective role of REHbβP in epididymal epithelial cells (EPEC's) led us to investigate: (1) the identification of LPS interactive domain in REHbβP, and (2) whether the REHbβP of rabbit origin mediates vaginal cellular immune responses of another species (human). HeLa-S3, human vaginal epithelial cells (hVECs) were exposed to LPS or the LPS-stimulated cells treated with REHbβP or neutral peptide, nREHbβP. Effect of LPS and cytokines (IL-6 and IL-1α) and chemokines (IL-8, MCP-1) levels was determined in the culture supernatants. In response to the LPS, hVECs synthesized these mediators and the levels were significantly higher than controls. This enhancing effect was ameliorated when the LPS-induced hVECs were treated with REHbβP. Similar results were obtained on NF-κB protein and hBD-1 mRNA expression. Confocal microscopy studies revealed that REHbβP attenuated the LPS-induced internalization of E. coli by macrophages. The chemotaxis studies performed using Boyden chamber Transwell assay, which showed elevated migration of U937 cells when the supernatants of LPS-induced hVECs were used, and the effect was inhibited by REHbβP. REHbβP was found to be localized on the acrosome of rabbit spermatozoa, suggesting its role in sperm protection beside sperm function. In conclusion, REHbβP may have the potential to develop as a therapeutic agent for reproductive tract infections (RTI's).

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“…A number of microorganisms infect reproductive tract in humans and animals, with serious consequences for reproductive function [3]. In men, the penile urethra is the portal of entry for pathogens (eg: Neisseria gonorrhoeae and Chlamydia trachomatis) and urethritis is the most common clinical syndrome [4]. Occasionally, these pathogens can also ascend into deeper organs such as the epididymis [4].…”

Section: Introductionmentioning

confidence: 99%

“…In men, the penile urethra is the portal of entry for pathogens (eg: Neisseria gonorrhoeae and Chlamydia trachomatis) and urethritis is the most common clinical syndrome [4]. Occasionally, these pathogens can also ascend into deeper organs such as the epididymis [4]. Retrograde infection of the epididymis may also occur from microorganisms present in the vas deferens, or via the blood vessels supplying epididymis.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that EPECs of rat, mice and human synthesize molecules having antimicrobial activities. Some of them are Bin-1β [9], Eppin [8] , SPAG-11 [2], HE2-α [10], β-defensins [2,11], SOB3 [12], EP2/HE2 [4], hCAP-18 [13], and cystatin-11 [14] etc. Despite the progress made in other species, the repertoire of host defense molecules also known as antimicrobial peptides (AMPs) having microbicidal activities have not been investigated previously in the epididymis of rabbit, Oryctologus cuniculus.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Molecules Having Anti-microbial Activities from Rabbit Epididymis Using Proteomic Approach

K.V.R¹,

Deva²,

Aranha³

et al. 2011

J Microb Biochem Technol

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Antimicrobial peptides (AMPs) are the key components of the host innate immune system and form the first line of host defense against invading pathogens. In the present study attempts were made to identify and characterize AMPs that are synthesized in the epididymal epithelial cells (EPECs) of rabbit, Oryctologus cuniculus. Ex-vivo induction of EPECs with TLR-9 ligand, CpG-ODN-2006 resulted an up-regulation of several AMPs. These molecules were identified in the acid extracts of pooled epididymal tissue homogenates using proteomic approach (Ultrafiltration, cation-exchange chromatography, RP-HPLC, Far Western blotting -FWB, 2D-PAGE and MALDI-TOF-MS). The pooled RP-HPLC fraction (peaks 1-5), named as rabbit epididymal secretory antimicrobial protein (RESAMP), showed activity against several pathogens. FWB, 2D-PAGE and MALDI-TOF-MS results revealed that of the ~19 proteins identified, four protein sequences (hemoglobin-α/β subunits, transthyretin and calreticulin) found to be reacted with HIV antigens (gp120, gp17, gp41, p24). One of the four sequences (VLSHHFGKEFTPQVQ) showed >90% homology with hemohlobin-β protein. A 29 mer peptide with VLSHHFGKEFTPQVQ sequence designated as rabbit epididymal hemoglobin beta (REHbβP). The synthesized peptide showed the highest Probability Based Mowse Score (PBMS) and demonstrated good bactericidal activity (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes and Candida albicans. REHbβP did not show cytotoxicity against human endocervical cells (End1/E6E7) and rabbit erythrocytes. Immunofluorescent results revealed that REHbβP localized in the stereocilia epididymis and on the acrosome of ejaculated spermatozoa of rabbit. To the best our knowledge, this is the first study describing the detection of AMPs in rabbit epididymis. In conclusion, the present study suggests that EPECs derived REHbβP may have therapeutic potential in the management of reproductive well being of human and animals.

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Cationic peptide of the male reproductive tract, HE2α, displays antimicrobial activity against Neisseria gonorrhoeae, Staphylococcus aureus and Enterococcus faecalis

Cited by 21 publications

References 10 publications

In Vitro Activities of Panduratin A against Clinical Staphylococcus Strains

In Vitro Activities of Panduratin A against Clinical Staphylococcus Strains

Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal CellsIn Vitro

Identification and Characterization of Molecules Having Anti-microbial Activities from Rabbit Epididymis Using Proteomic Approach

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