Cationic Transfection Lipids

Liu, Dexi; Ren, Tanchen; Gao, Xiang

doi:10.2174/0929867033457386

Cited by 86 publications

(58 citation statements)

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“…[56] In this work, two malonic acid amides (lipids 7 and 8) characterized by a hydrophilic head group composed of lysine linked via ethylenediamine were used. The two lipids vary only in the alkyl chain pattern.…”

Section: Discussionmentioning

confidence: 99%

Structure–Function Relationships of New Lipids Designed for DNA Transfection

et al. 2011

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Cationic liposome/DNA complexes can be used as nonviral vectors for direct delivery of DNA-based biopharmaceuticals to damaged cells and tissues. To obtain more effective and safer liposome-based gene transfection systems, two cationic lipids with identical head groups but different chain structures are investigated with respect to their in vitro gene-transfer activity, their cell-damaging characteristics, and their physicochemical properties. The gene-transfer activities of the two lipids are very different. Differential scanning calorimetry and synchrotron small- and wide-angle X-ray scattering give valuable structural insight. A subgel-like structure with high packing density and high phase-transition temperature from gel to liquid-crystalline state are found for lipid 7 (N'-2-[(2,6-diamino-1-oxohexyl)amino]ethyl-2,N-bis(hexadecyl)propanediamide) containing two saturated chains. Additionally, an ordered head-group lattice based on formation of a hydrogen-bond network is present. In contrast, lipid 8 (N'-2-[(2,6-diamino-1-oxohexyl)amino]ethyl-2-hexadecyl-N-[(9Z)-octadec-9-enyl]propanediamide) with one unsaturated and one saturated chain shows a lower phase-transition temperature and a reduced packing density. These properties enhance incorporation of the helper lipid cholesterol needed for gene transfection. Both lipids, either pure or in mixtures with cholesterol, form lamellar phases, which are preserved after addition of DNA. However, the system separates into phases containing DNA and phases without DNA. On increasing the temperature, DNA is released and only a lipid phase without intercalated DNA strands is observed. The conversion temperatures are very different in the two systems studied. The important parameter seems to be the charge density of the lipid membranes, which is a result of different solubility of cholesterol in the two lipid membranes. Therefore, different binding affinities of the DNA to the lipid mixtures are achieved.

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Section: Discussionmentioning

confidence: 99%

Structure–Function Relationships of New Lipids Designed for DNA Transfection

et al. 2011

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“…Cationic liposomes condense nucleic acids into lipid-nucleic acid complexes (lipoplexes) that interact with the negatively charged cell membrane (Liu et al, 2003). Although several groups have published technical papers showing that liposomes can work in vivo, they are still not commonly used.…”

Section: Introductionmentioning

confidence: 99%

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

et al. 2011

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Here we report that highly concentrated cationic lipid/helper lipid‐nucleic acid complexes (lipoplexes) can facilitate reproducible delivery of a variety of oligonucleotides and plasmids to chicken embryos or to mouse embryonic mesenchyme. Specifically, liposomes composed of N,N‐dioleyl‐N,N‐dimethylammonium chloride (DODAC)/1,2 dioleoyl glycero‐3‐phosphorylethanolamine (DOPE) prepared at 18‐mM concentrations produced high levels of transfection of exogenous genes in vivo and in vitro. Furthermore, we report sufficient uptake of plasmids expressing interference RNA to decrease expression of both exogenous and endogenous genes. The simplicity of preparation, implementation, and relatively low toxicity of this transfection reagent make it an attractive alternative for developmental studies in post‐gastrulation vertebrate embryos. Developmental Dynamics 240:2108–2119, 2011. © 2011 Wiley‐Liss, Inc.

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“…Clinical development of gene therapy requires the introduction of genes into target cells which has been achieved using synthetic vehicles of high safety, low immunogenicity and ease of manufacture [1][2][3]. Taking advantage of DNA self-assembly with a cationic polymer such as polylysine or polyethylenimine to condense a plasmid DNA like the viral genome in viral particles, such electrostatic complexes (termed polyplexes) are prospected for the design of synthetic gene delivery systems that incorporate viral-like features for efficient transfection.…”

Section: Introductionmentioning

confidence: 99%