CatSper: The complex main gate of calcium entry in mammalian spermatozoa

Rahban, Rita; Nef, Serge

doi:10.1016/j.mce.2020.110951

Cited by 48 publications

(46 citation statements)

References 125 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Egg activation comprised a temporal serious of [Ca 2+ ]i dependent events, that include cortical granule exocytosis, cell cycle resumption with change of MPF/MAP kinase activities, and recruitment of maternal mRNAs (Ducibella et al, 2002;Ajduk et al, 2008). In this study, we used 10 mM SrCl 2 to induce Ca 2+ oscillation and to eliminate the effect of VDCCs inhibitor on sperm physiology (Rahban & Nef, 2020). The [Ca 2+ ]i oscillation pattern of ω-Conotoxin CnVIIA is different compared to the control.…”

Section: Discussionmentioning

confidence: 99%

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

et al. 2020

View full text Add to dashboard Cite

Repetitive changes in the intracellular calcium concentration ([Ca 2+ ]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca 2+ ]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca 2+ ion elevation during [Ca 2+ ]i oscillations are Ca 2+ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca 2+ ion influx through Ca 2+ channel on the plasma membrane. Ca 2+ channels have been characterized into voltage-dependent Ca 2+ channels (VDCCs), ligand-gated Ca 2+ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca 2+ ]i oscillation was observed in a Ca 2+ contained medium with sperm factor or adenophostin A injection but disappeared in Ca 2+ free medium. Ca 2+ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca 2+ ]i oscillation profiles in SrCl 2 treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca 2+ influx is essential for [Ca 2+ ]i oscillation during mammalian fertilization. This Ca 2+ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.

show abstract

Section: Discussionmentioning

confidence: 99%

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

et al. 2020

View full text Add to dashboard Cite

show abstract

“…On the other hand, no study has been conducted about the effect of propranolol on CatSper gene and protein expression. The CatSper gene family contains CatSper 1-4 and beta and gamma [9]. CatSper genes are the key genes in male fertility expressed exclusively in the testes [10].…”

Section: Propranololmentioning

confidence: 99%

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Mohammadi

Beheshti

Elyasi

et al. 2020

JPRI

View full text Add to dashboard Cite

The aim of this study was to investigate the effects of propranolol on sperms, histopathology of testes, and CatSper 2 gene and protein expression in adult mice. 18 adult male mice were randomly divided into control, propranolol 1 (receiving 10 mg/kg dose) and propranolol 2 group (receiving 15 mg/kg dose for 35 days). The mean amount of sperm parameters in the propranolol 1 and propranolol 2 groups was significantly lower than the control group (p<0.05). CatSper2 gene and protein expression have significantly decreased in propranolol 1 and propranolol 2 groups compared to the control group (P<0.05). Reduction of CatSper2 gene and protein expression in low dose of propranolol was more severe than high dose. In testicular tissues of the propranolol 1 group, vacuoles and necrosis in the germinal epithelium were observed, and in testicular tissues of propranolol 2 group decrease in the thickness of the germinal epithelium, some vacuoles and necrosis were observed in germinal epithelium as well as congestion in the interstitial space. The mean value of thiol and catalase enzyme in the propranolol 1 and propranolol 2 groups, and the mean value of superoxide dismutase in propranolol 1 group, were significantly different compared to the control group (P<0.05).

show abstract

“…Studies have shown that the hormones progesterone and prostaglandin in the reproductive system regulate the physiological function of human spermatozoa mainly through changes in Ca 2þ concentration mediated by CatSper channels. 14 In addition, many reports indicate that some synthetic fragrances and a variety of environmental endocrine disruptors can affect the physiological function of human sperm by activating CatSper channels. 12,14 Progesterone directly activates the spermatozoa-specific calcium channel CatSper; in addition, it induces extracellular calcium influx and ultimately regulates spermatozoa-related physiological functions.…”

Section: Introductionmentioning

confidence: 99%

“…14 In addition, many reports indicate that some synthetic fragrances and a variety of environmental endocrine disruptors can affect the physiological function of human sperm by activating CatSper channels. 12,14…”

Section: Introductionmentioning

confidence: 99%

Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men

et al. 2021

View full text Add to dashboard Cite

Objective This was a prospective study to investigate whether progesterone affects sperm activity by regulating the cyclic AMP-protein kinase A (cAMP-PKA) signalling pathway via α/β hydrolase domain-containing protein 2 (ABHD2). Methods Spermatozoa were collected from healthy and infertile men (with oligoasthenospermia or abnormal acrosome; n = 30/group). The expression of and mutations in ABHD2 were detected by quantitative PCR, western blot, and gene sequencing. The expression of ABHD2 in the presence of progesterone was detected in all groups, and cAMP and PKA levels were detected by ELISA in fertile men after treatment with ABHD2 antibody and PKA inhibitor H-89, respectively. Results Expression of ABHD2 mRNA and protein were reduced in spermatozoa from infertile compared with fertile men. Four gene mutation sites were detected in spermatozoa from the infertile groups. Progesterone increased mRNA and protein levels of ABHD2 in healthy spermatozoa but not in spermatozoa from infertile men. The levels of cAMP and PKA were increased by progesterone in healthy spermatozoa, and the progesterone-increased cAMP and PKA were decreased by ABHD2 antibody and H-89, respectively. Conclusion Progesterone regulates the ABHD2-mediated cAMP-PKA signalling pathway in healthy spermatozoa, which provides a new target for clinical diagnosis and treatment of infertility.

show abstract

CatSper: The complex main gate of calcium entry in mammalian spermatozoa

Cited by 48 publications

References 125 publications

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization

The Effect of Propranolol on Sperm Parameters, CatSper 2 Gene and Protein Expression, and Oxidative Stress in Adult Mice

Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men

Contact Info

Product

Resources

About