Cattle breed affects in vitro embryo production in a large-scale commercial program on dairy farms

Lacerda, Izabelle Pereira de; Dode, M. A. N.; Lima, Marcelo Machado Souza; Guerra, Breno Fonseca; Costa, Eduardo Silva; Moreira, Gisele Rodrigues; Carvalho, José de Oliveira

doi:10.1016/j.livsci.2020.104135

Cited by 7 publications

(4 citation statements)

References 51 publications

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“…On the other hand, in dairy breeds: Gir (Bos indicus) and Holstein (Bos taurus), a difference was observed in oocyte recovery (P <0.05), corroborating with other studies also carried out in Gir and Holstein cows, in which Gir donors showed better results in oocyte recovery (Pontes et al, 2010;Silva et al, 2015). These results were already expected, as Bos indicus cows generally exhibit three follicular waves (Baruselli, Gimenes, & Sales, 2007;Silva-Santos et al, 2011), consequently having more ovarian follicles, resulting in a greater number of oocytes recovered by OPU (Lacerda et al, 2020;Viana et al, 2004;Xu et al, 2006).…”

Section: Discussionsupporting

confidence: 86%

“…This variability may be related to differences in sperm quality (Alomar et al, 2006;Gillan et al, 2008), microRNA expression (Govindaraju et al, 2012), sperm plasma membrane protein (Gaviraghi et al, 2010;Roncoletta et al, 2006) and sperm gene expression (Khatib et al, 2009). However, data indicate that after fertilization with sperm having intact DNA, part of development is controlled by the oocyte, including activation of the embryonic genome (Heikinheimo & Gibbons, 1998), indicating that the effect of donor breed on embryo production is more relevant than bull breed (Lacerda et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Comparison between in vitro embryo production in Bos indicus and Bos taurus cows

Moschini

Gaitkoski²,

Almeida

et al. 2021

RSD

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The aim of this study was to evaluate the in vitro embryo production in Bos taurus and Bos indicus donors, evaluating from oocyte recovery to final embryo production. Data were collected during the months of January and February and August to October 2017. Bos taurus cows: Holstein and Senepol and Bos indicus cows: Nelore and Dairy Gir, were used as oocyte donors. In total, 3622 sessions of OPU were performed, divided between: Holstein donors: 925; Senepol donors: 1055; Nelore donors: 1202 and Dairy Gir donors: 440. In total, 103.399 oocytes were recovered, being viable oocytes: 90.807; cleaved: 65.252 and produced embryos: 24216. There was no statistical difference between the genetic groups for the total oocytes recovered (Bos taurus: 25.5 vs. Bos indicus: 30.1; P> 0.05). However, a statistical difference was observed between the cleaved (Bos taurus: 16 vs. Bos indicus: 20.4; P <0.05) and produced embryos (Bos taurus: 4.4 vs. Bos indicus: 8.3; P <0,05). Thus, the conclusion is that Zebu donors perform better in IVEP and the donor breed influences the IVEP results.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Comparison between in vitro embryo production in Bos indicus and Bos taurus cows

Moschini

Gaitkoski²,

Almeida

et al. 2021

RSD

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“…This is comparable to some extend with the 15-31% in vitro embryo production from data compiled from the American Embryo Transfer Association (AETA) [14] and the 20-32% in vitro blastocyst production reported from data generated at Brazilian farms [17]. The variation in bovine IVEP efficiency is multifactorial, including factors such as lactational status [18], oocyte donor age [16,19,20] and breed [21][22][23][24], bull (including bull breed) [17,25,26], heat stress [27], interval between OPU sessions [28], use of ovarian stimulation [14,29], inherent antral follicle number [30,31], culture media [32,33], equipment [34][35][36], and nutrition of the oocyte donor [37,38]. Of these factors, the nutritional status of oocyte donors has been investigated substantially given the critical role that nutrition plays in fertility.…”

Section: Introductionsupporting

confidence: 71%

Nutritional Strategies to Promote Bovine Oocyte Quality for In Vitro Embryo Production: Do They Really Work?

Velazquez

2023

Veterinary Sciences

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The ability of bovine oocytes to reach the blastocyst stage (i.e., embryo with around 150 cells in cattle) in vitro can be affected by technical (e.g., culture medium used) and physiological factors in oocyte donors (e.g., age, breed). As such, the nutritional status of oocyte donors plays a significant role in the efficiency of in vitro embryo production (IVEP), and several nutritional strategies have been investigated in cattle subjected to ovum pick-up (OPU). However, there is no clear consensus on the reliability of nutritional schemes to improve IVEP in cattle. Available evidence suggests that a moderate body condition score (i.e., 3 in a 1–5 scale) in cattle is compatible with a metabolic microenvironment in ovarian follicles that will promote embryo formation in vitro. The usefulness of fatty acid and micronutrient supplementation to improve IVEP in cattle is debatable with the current information available. Overall, the supply of maintenance nutritional requirements according to developmental and productive stage seems to be enough to provide bovine oocyte donors with a good chance of producing embryos in vitro. Future nutrition research in cattle using OPU-IVEP models needs to consider animal well-being aspects (i.e., stress caused by handling and sampling), which could affect the results.

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“…Bos indicus has more significant retrieved oocytes due to a greater antral follicle count than Bos taurus (Sartori et al 2016). They can adapt to heat stress and humidity, rusticity, resistance to endo and ectoparasites (Porto-Neto et al 2014), poor pasture quality, and a tendency for larger oocytes (de Vasconcelos et al 2020;de Lacerda et al 2020;Oliveira et al 2019;Sales et al 2015;Guerreiro et al 2014). However, Bos taurus has been subjected to more extensive selection and generally has better productivity, including early maturity (Coffey et al 2016;Madalena et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

Irma,

Rasad,

Hilmia

et al. 2023

JITV

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Belgian Blue was introduced in Indonesia to increase the biodiversity of livestock genetic resources. Â Belgian Blue was crossed with Ongole grade to increase the productivity of local cattle.Â Therefore, this study evaluates reproduction traits, especially the response to superovulation, embryonic development, and quality of Belgian Blue, Ongole grade, and their crosses.Â Estrous was synchronized with intravaginal progesterone Cue-Mate before artificial insemination (AI).Â Superovulation was performed with Follicle Stimulating Hormone (FSH) intramuscularly with non-surgical embryo flushing. Â In addition, embryo quality was assessed microscopically according to the International Embryo Transfer Society (IETS) criteria.Â The study was performed in a quasi-experimental design, and data were analyzed with an analysis of variance.Â After superovulation, oocytes/embryos were obtained from all donor breeds.Â Oocyte and embryo production from Ongole grade and Belgian Blue differed at 11.83Â±1.91 and 4.86Â±1.33, respectively, P0.05 (meanÂ±SEM).Â In addition, there are differences in recovery rate (89.63% vs. 75.35%) and fertilization rate (77.35% vs. 68.22%) between Ongole grade and Belgian Blue, respectively (P0.05).Â There is no difference in embryo development quality and proportion of transferable embryos between Ongole grade, Belgian Blue, and their crosses.Â This study concluded that the cross-bred Belgian Blue x Ongole donor had identical oocyte and embryo production, recovery rate, fertilization rate, and degenerative embryos compared to its purebred.

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Cattle breed affects in vitro embryo production in a large-scale commercial program on dairy farms

Cited by 7 publications

References 51 publications

Comparison between in vitro embryo production in Bos indicus and Bos taurus cows

Comparison between in vitro embryo production in Bos indicus and Bos taurus cows

Nutritional Strategies to Promote Bovine Oocyte Quality for In Vitro Embryo Production: Do They Really Work?

Embryo Production and Development from Superovulated Donors in Double-Muscled Cattle and Their Crosses

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