Cauliflower is a New Host of a Subgroup 16SrVII-B Phytoplasma Associated with Stunting Disease in Brazil

Pereira, Thays Benites Camargo; Dally, E. L.; Davis, Robert E.; Banzato, Ticyana Carone; Galvão, Sarah Rodrigues; Bedendo, Ivan Paulo

doi:10.1094/pdis-09-15-1110-pdn

Cited by 11 publications

(2 citation statements)

References 5 publications

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“…This is proven by a stunt disease in broccoli that may be caused by phytoplasma groups 16SrI, 16SrIII, or 16SrXII (Eckstein et al, 2013). Additionally, phytoplasma contamination of vegetable crops may be associated with non-specific symptoms such as leaf yellowing, reddening, curling, vein clearing, stunting, and fruit deformity (Pereira et al, 2016).…”

Section: Stages Of Symptomsmentioning

confidence: 99%

Global Status of Phytoplasma Associated Diseases on Different Ornamental Crops. A Review of Recent Research

Ahmad¹,

Khan²

2021

PLANT ARCHIVES

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This paper reviews the global status of phytoplasma disease associated with different ornamental crops. This paper investigated phytoplasmas, an equally important group of pathogens that severely harm the growth and marketing properties of ornamental plants, lowering their commercial worth. In this paper, number of ornamental plants was found to be infected with 'Ca. Phytoplasma' species and treatment techniques were adapted using tetracycline insecticides for elimination and emission of phytoplasmas.

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Section: Stages Of Symptomsmentioning

confidence: 99%

Global Status of Phytoplasma Associated Diseases on Different Ornamental Crops. A Review of Recent Research

Ahmad¹,

Khan²

2021

PLANT ARCHIVES

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“…(Fugita et al 2017). Specifically regarding subgroup 16SrVII-B, the reference phytoplasma of this subgroup was described from erigeron and periwinkle (Barros et al 2002), while other members of this subgroup were reported in cauliflower (Pereira et al 2016a) and ming aralia (Pereira et al 2016b).…”

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confidence: 99%

Olive Tree Represents a New Host of a Subgroup 16SrVII-B Phytoplasma Associated with Witches’ Broom Disease in Brazil

et al. 2021

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Olive trees exhibiting slow development, yellowing, and high intensity of shoot proliferation with small leaves were observed in commercial plantings, in the municipality of Extrema, Minas Gerais (MG) state in 2015. The incidence of symptomatic plants was about 70% and diseased trees presented yield reduction. Here we report the association of symptomatic olive trees with a phytoplasma and describe its molecular identification. Symptomatic plants (38 trees) were sampled in three growing areas located in the same municipality. The samples consisted of bunch of leaves and young shoots. The total DNA was extracted using DNeasy® Plant Mini Kit (Qiagen, Hilden, Germany). Phytoplasma detection was conducted by nested PCR with primers P1/16S-SR (Lee et al. 2004) followed by R16F2n/R16R2 (Gundersen and Lee 1996). PCR assays generated amplicons (~1.2 kb) from 28 trees out of 38 symptomatic plants, confirming the association of phytoplasma with diseased plants. The disease was named olive witches’ broom. The genomic fragments amplified by nested PCR were cloned into Escherichia coli DH5α and sequenced. The sequence representative of the olive phytoplasma was designated OWB-Br01 (Olive Wiches' Broom-Brazil 01) and deposited in GenBank under accession number MH141985. This sequence shared 99% sequence identity with phytoplasmas affiliated with 16SrVII group. According to the iPhyClassifier online tool (Zhao et al. 2009) the olive witches’-broom phytoplasma was classified as a variant of subgroup 16SrVII-B with a pattern similarity coefficient of 0.99. The phylogenetic tree showed that OWB-Br01 phytoplasma emerges from the same branch of the reference phytoplasma of the 16SrVII-B subgroup (Erigeron witches᾽-broom phytoplasma - GenBank AY034608), indicating that the olive tree phytoplasma is a member of the 16SrVII-B subgroup. The pathogenicity test was performed with 28 healthy plants (cultivar Arbequina) grown in pots, which were grafted by simple english forklift with scions obtained from olive plants (Arbequina) six years old, naturally infected by the phytoplasma. The initial symptoms were observed four months after grafting and at eight months 22 grafted plants exhibited slow growth, yellowing, and small leaves as those naturally observed in the fields. Molecular characterization allowed identify the phytoplasma as a member of the 16SrVII-B subgroup. In Brazil, representatives of the 16SrVII group were previously reported in association with diverse botanical species. Thus, a strain of 16SrVII-C subgroup was identified in sunn hemp (Flôres et al. 2013); the reference phytoplasma of 16SrVII-D subgroup was found in erigeron plants (Flôres et al. 2015); and the representative of 16SrVII-F was detected in the wild species Vernonia brasiliana. (Fugita et al. 2017). Specifically regarding subgroup 16SrVII-B, the reference phytoplasma of this subgroup was described from erigeron and periwinkle (Barros et al. 2002), while other members of this subgroup were reported in cauliflower (Pereira et al. 2016a) and ming aralia (Pereira et al. 2016b). The disease here studied is a threat since olive planting is in large expansion in Brazil. A potential control option could be use of propagative material from sources free of the pathogen. Based on our findings, olive tree represents a new host for subgroup 16SrVII-B phytoplasma, which is different from 16Sr groups previously reported as associated with olive witches’ broom in other countries.

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Impact and Management of Major Phytoplasma Diseases in Brazil

Bedendo

Lopes

2019

Sustainability in Plant and Crop Protection

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Cauliflower is a New Host of a Subgroup 16SrVII-B Phytoplasma Associated with Stunting Disease in Brazil

Cited by 11 publications

References 5 publications

Global Status of Phytoplasma Associated Diseases on Different Ornamental Crops. A Review of Recent Research

Global Status of Phytoplasma Associated Diseases on Different Ornamental Crops. A Review of Recent Research

Olive Tree Represents a New Host of a Subgroup 16SrVII-B Phytoplasma Associated with Witches’ Broom Disease in Brazil

Impact and Management of Major Phytoplasma Diseases in Brazil

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