Rearing insects for pest experiment it’s very critical. The condition of rearing such insects impacted the smooth running of research. This condition also happens in the rearing of Asian Citrus Psyllid (ACP), Diaphorina citri. ACP is one of the most dangerous pests of citrus since responsible for the huanglongbing disease vector in citrus. Many researchers, either laboratory or field-based use a D.citri for object experiments. A clear and recent description of the types of insects or arthropods that disturb the rearing ACP is needed, especially in Indonesia. The research was conducted in the greenhouse of Faculty of Agriculture, Gadjah Mada University, from January 2021 to September 2022. The rearing of ACP uses orange jasmine (Muraya paniculata) and citrus (Citrus nobilis). The collected insect and spider preserved in alcohol or pined in insect tray and identify them based on related literature. This study proposed to assess the nuisance insect that infested on rearing of D.citri based on greenhouse conditions. Our results showing that the many nuisance insects that disturb the growth of rearing in D.citri. A total of nine insect and spider attacking D citri, they are black ants (Dolichoderus sp), aphid (Aphis sp), leaf roller caterpillar (Phyllocnistis citrella), cockroach (Blattella sp), ladybug (Exochomus nigromaculatus), mite (Tetranychus sp), Mealy bug (Pseudoccocus sp), Scale insect (Aonidella aurantia) and Spider. Those nuisance insects and spiders attack D.citri colonies in different ways and stages. The attacking nuisance insect affected to affected the size of the ACP culture.