Causes of Imposex in the Dogwhelk Nucella lapillus (L.) and its Use as a Biological Indicator of Tributyltin Contamination

Evans, Sue; Kerrigan, E.; Palmer, Norman D.

doi:10.1016/s0025-326x(99)00210-6

Cited by 54 publications

(28 citation statements)

References 39 publications

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“…Due to the nonspecific effects of heavy metal leaching, such paints are environmentally hazardous. An example is the very effective toxic compound tributyl tin, which is believed to contribute to the development of antimicrobial tolerance in marine organisms (35) and to cause imposex in some invertebrates (1,16). Antifouling paints containing tin have been banned since 2003 and gradually removed from shipping fleets (27).…”

mentioning

confidence: 99%

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

Bernbom

Kjelleberg

et al. 2011

Appl Environ Microbiol

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The aims of this study were to determine if marine bacteria from Danish coastal waters produce antifouling compounds and if antifouling bacteria could be ascribed to specific niches or seasons. We further assess if antibacterial effect is a good proxy for antifouling activity. We isolated 110 bacteria with anti-Vibrio activity from different sample types and locations during a 1-year sampling from Danish coastal waters. The strains were identified as Pseudoalteromonas, Phaeobacter, and Vibrionaceae based on phenotypic tests and partial 16S rRNA gene sequence similarity. The numbers of bioactive bacteria were significantly higher in warmer than in colder months. While some species were isolated at all sampling locations, others were niche specific. We repeatedly isolated Phaeobacter gallaeciensis at surfaces from one site and Pseudoalteromonas tunicata at two others. Twenty-two strains, representing the major taxonomic groups, different seasons, and isolation strategies, were tested for antiadhesive effect against the marine biofilm-forming bacterium Pseudoalteromonas sp. strain S91 and zoospores of the green alga Ulva australis. The antiadhesive effects were assessed by quantifying the number of strain S91 or Ulva spores attaching to a preformed biofilm of each of the 22 strains. The strongest antifouling activity was found in Pseudoalteromonas strains. Biofilms of Pseudoalteromonas piscicida, Pseudoalteromonas tunicata, and Pseudoalteromonas ulvae prevented Pseudoalteromonas S91 from attaching to steel surfaces. P. piscicida killed S91 bacteria in the suspension cultures, whereas P. tunicata and P. ulvae did not; however, they did prevent adhesion by nonbactericidal mechanism(s). Seven Pseudoalteromonas species, including P. piscicida and P. tunicata, reduced the number of settling Ulva zoospores to less than 10% of the number settling on control surfaces. The antifouling alpP gene was detected only in P. tunicata strains (with purple and yellow pigmentation), so other compounds/mechanisms must be present in the other Pseudoalteromonas strains with antifouling activity.

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confidence: 99%

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

Bernbom

Kjelleberg

et al. 2011

Appl Environ Microbiol

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“…Ten-, 20-and 100-fold diluted NB (NB/10, NB/20 and NB/100, respectively) was prepared with freshly distilled water. The MS medium contained (per liter): 0.5 g Na 2 SO 4 O. Yeast extract (Difco Laboratories, Detroit, MI) was added to the medium (quantities as indicated in the results section). The pH of the medium was adjusted to 7.0 with 1 M NaOH.…”

Section: Mediamentioning

confidence: 99%

“…During the last decade, concern has been mounting over NP, since this compound is known to be a member of the group of endocrine disruptors, more specifically pseudoestrogens. Evidence is emerging that NP accumulates in organisms, and produces several adverse effects, including sexchange and reduced fertility 2,4,14,15,19,25,28,34) .…”

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confidence: 99%

Organic Nutrient-dependent Degradation of Branched Nonylphenol by Sphingomonas sp. YT Isolated from a River Sediment Sample.

et al. 2001

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A conventional enrichment culture on branched nonylphenol (NP) with diluted nutrient broth as an additional source of organic nutrients yielded a bacterial strain able to degrade branched NP. The isolate (designated YT) was identified as Sphingomonas sp. based on an analysis of its 16S ribosomal RNA genes and cellular lipids. The degradation of NP by strain YT occurred primarily during the exponential phase of cell growth in cultures on a yeast extract-mineral salts medium. The degree of degradation was directly proportional to the amount of yeast extract present in the medium and no significant growth occurred when NP was the sole source of carbon and energy. Gas chromatography-mass spectrometry (GC-MS) of resting cell suspensions incubated with branched NP revealed that the degradation did not yield any metabolites containing aromatic residues but only branched alcohols. When a linear NP was used as the target substrate, GS-MS of the suspensions indicated the appearance of a hydroxylated linear NP as an intermediate during the degradation. Strain YT is expected to attack NP by an initial oxidative cleavage of the phenol ring.

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“…However the amounts stored in the sediments, those from early applications and a possible illegal use render these compounds still available to organisms. Even though organotin compounds, were considered the main cause of imposex, recently, hypotheses about the involvement of other factors have been suggested (Garaventa et al, 2006b); Nias et al (1993) observed that copper and environmental stress can cause imposex in Lepsiella vinosa (Lamarck, 1822); Evans et al (2000) showed that nonylphenol promotes the phenomenon in N. lapillus. Recently an epidemiologic study conducted by Maran et al (2006) suggested that organochlorine pesticides, polychlorinated biphenyls and, at a lower extent, polycyclic aromatic hydrocarbons can have a role in the imposex development.…”

Section: Introductionmentioning

confidence: 99%

Imposex levels and concentrations of organotin compounds (TBT and its metabolites) in Nassarius nitidus from the Lagoon of Venice

Pavoni

Centanni

Valcanover

et al. 2007

Marine Pollution Bulletin

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Specimens of Nassarius nitidus were collected in seven stations of the Venice Lagoon to assess the levels of tributyltin (TBT) and its metabolites monobutyltin and dibutyltin in the tissues and monitor their effect on organisms, in particular the phenomenon of imposex (superimposition of male sexual characteristics on females). The following values of population indices were found: vas deferens sequence: 1.2 ± 0.7-4.0 ± 0.5; relative penis length: 6-47%. The least impacted station was situated in the northern part of the Lagoon, where females without imposex were found and Butyltin (BuTs) concentrations in the organisms (average sum of BuTs = 43 ± 14 ng Sn g À1 w.) were significantly lower than in the other stations (range of average sum of BuTs: 101 ± 22-217 ± 27 ng Sn g À1 d.w.). Population indices were found to be related to the TBT content in the tissues. In particular VDSI had a significant logarithmic correlation: r = 0.95, n = 8, p < 0.05.

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Causes of Imposex in the Dogwhelk Nucella lapillus (L.) and its Use as a Biological Indicator of Tributyltin Contamination

Cited by 54 publications

References 39 publications

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

Marine Bacteria from Danish Coastal Waters Show Antifouling Activity against the Marine Fouling Bacterium Pseudoalteromonas sp. Strain S91 and Zoospores of the Green Alga Ulva australis Independent of Bacteriocidal Activity

Organic Nutrient-dependent Degradation of Branched Nonylphenol by Sphingomonas sp. YT Isolated from a River Sediment Sample.

Imposex levels and concentrations of organotin compounds (TBT and its metabolites) in Nassarius nitidus from the Lagoon of Venice

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