Cautionary Notes on Use of the MoT3 Diagnostic Assay for <i>Magnaporthe oryzae</i> Wheat and Rice Blast Isolates

Gupta, Dipali Rani; Avila, Claudia Sarai Reyes; Win, Joe; Soanes, Darren M.; Ryder, Lauren S.; Croll, Daniel; Bhattacharjee, Pallab; Hossain, Shaid; Mahmud, Nur Uddin; Mehebub, Md. Shabab; Surovy, Musrat Zahan; Rahman, Mahbubur; Talbot, Nicholas J.; Kamoun, Sophien; Islam, Tofazzal

doi:10.1094/phyto-06-18-0199-le

Cited by 28 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since M. oryzae is a species complex and disease symptom of wheat blast is always confused with that caused by Fusarium head blight, it is therefore important to diagnose the disease accurately to apply suitable control measure timely. Interestingly, our previous results using M. oryzae isolates from rice and wheat blast showed that MoT3 primers were not specific for MoT isolates under certain conditions (Gupta et al 2019). This result was contradictory with that obtained by Pieck et al (2017).…”

Section: Discussionmentioning

confidence: 76%

“…In this study, pot2-transposon specific primers were used to amplify Pot2 region that is thought to be present in isolates of M. oryzae and M. grisea from different hosts (Harmon et al 2003). A recently developed primer by Pieck et al (2017) named MoT3 was also used, which is unique for identification of isolates of M. oryzae Triticum pathotype when used under stringent conditions (Gupta et al 2019).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

et al. 2020

Self Cite

View full text Add to dashboard Cite

Wheat blast disease caused by a South American lineage of Magnaporthe oryzae Triticum (MoT) pathotype has emerged as a serious threat to wheat production in Bangladesh since its first emergence in 2016. Efficient and suitable methods for isolation, storage, inoculum production and molecular characterization of the pathogen can help in achieving the target of sustainable management of the disease in a relatively short period of time. In this study, we aimed to develop suitable methods for isolation, storage and morphological characterization and molecular identification of MoT isolates collected from the blast-infected wheat fields in Bangladesh. This process included modification of existing protocols that were available for a related fungal pathogen M. oryzae or de novo method development and validation. We developed suitable methods for isolation of MoT from field-infected plant samples using modified monoconidial isolation technique and produced abundant conidia from a single mycelial plate for in vivo pathogenicity assay in a reproducible manner. Cultural and morphological characterization of the isolates revealed that all Bangladeshi MoT isolates are of a single clonal lineage with similar cultural and morphological characters. Molecular detection of isolates with M. oryzae-specific primers Pot1 and Pot2 and MoT-specific primers MoT3F and MoT3R produced bands with the expected size from all wheat-infecting isolates. We also successfully established a PCR-based detection system based on a commercially available detection kit for field-infected leaf and seed samples by detecting Pot2- and MoT3-specific bands. Additionally, the simple method we developed in our study for producing abundant conidia in a very short period of time will be very helpful in studying biology of the wheat blast fungus. This method was also proven to be more user-friendly and cost-effective than previously available methods. Successful characterization of MoT isolates at morphological and molecular levels coupled with detection of the pathogen in infected field and seed lots should be useful for efficient surveillance and management of the fearsome wheat blast disease.

show abstract

Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…This method may be useful in detecting trapped MoT spores from the aerosol samples and for the prediction of epidemics along the year. However, Gupta et al (2019) published a result contradictory to the previous result of Pieck et al (2017) as they observed that under certain conditions, MoT3 primers also amplify both rice and wheat blast isolates. The assay is based on primers matching the WB12 sequence corresponding to a fragment of the M. oryzae MGG_02337 gene that is annotated as a short chain dehydrogenase and is present in both rice and wheat infecting M oryzae isolates.…”

Section: Introductionmentioning

confidence: 69%

“…Although some diagnostic protocols have been developed for the wheat blast, they are not highly convenient and unequivocally reliable (Pieck et al 2017;Gupta et al 2019). Therefore, a more convenient, rapid and costeffective diagnostic tool needs to be developed for surveillance and monitoring of this wheat pathogen.…”

Section: Introductionmentioning

confidence: 99%

Wheat blast: a new threat to food security

et al. 2020

Self Cite

View full text Add to dashboard Cite

Wheat blast, caused by the Magnaporthe oryzae Triticum (MoT) lineage (synonym Pyricularia oryzae Triticum lineage), is a destructive disease in South America and Bangladesh. It is primarily a disease of wheat head, which can cause yield loss up to 100% under favorable disease conditions. The head infection results in complete or partial bleaching of the spike above the point of infection with either no grain or shriveled grain with low test weight. Due to low fungicide efficacy against the disease and lack of availability of resistant varieties, an integrated management program should be adopted to control this serious wheat disease. First of all, a convenient and specific diagnostic tool is needed for evaluating seed health and early detection in wheat field to initiate timely mitigation measures and thereby decreasing pathogen initial inoculum and dispersal. Second, we should have a better understanding of the epidemiology of the disease and develop a real-time disease monitoring and surveillance system to alert growers to apply management practices at an optimum time. Third, we need a better understanding of the infection biology of the fungus and its interaction with wheat plants at the tissue and molecular levels helpful for improving disease management. Fourth, breeding for resistance to wheat blast can be accelerated by using resistance genes such as 2NS translocation, Rmg8 and RmgGR119 or advanced genomic technology such as CRISPR-Cas. Fifth, integration of alternative disease management practices, such as biological control using antagonistic microorganisms or derivatives thereof to achieve sustainable approach for the management of wheat blast. Finally, a globally concerted effort is needed using open science and open data sharing approaches to prevent this seed-and airborne plant disease's widespread devastation of wheat crop. This comprehensive review updates our knowledge on wheat blast disease and discusses the approaches for its sustainable management for ensuring food and nutritional security of the ever-increasing global population.

show abstract

“…Considering the importance of wheat for food security in South Asia and India in particular, the present study, therefore, calls for concerted action from the national governments in South Asia and international stakeholders. Until now, there is no specific molecular diagnostic tool for the determination of wheat blast [63] in suspected seeds, alternative hosts and symptomless plants. There is an urgent need to develop a convenient diagnostic tool for wheat blast to support surveillance and to invest in disease epidemiology and forecasting research.…”

Section: Materials and Methods: Context And Ex-ante Estimation Proceduresmentioning

confidence: 99%

Averting wheat blast by implementing a ‘wheat holiday’: In search of alternative crops in West Bengal, India

et al. 2019

View full text Add to dashboard Cite

The emergence of wheat-blast in Bangladesh in the 2015–16 wheat ( Triticum aestivum L.) crop threatens the food security of South Asia. A potential spread of the disease from Bangladesh to India could have devastating impacts on India’s overall food security as wheat is its second most important staple food crop. West Bengal state in eastern India shares a 2,217 km-long border with Bangladesh and has a similar agro-ecology, enhancing the prospects of the disease entering India via West Bengal. The present study explores the possibility of a ‘wheat holiday’ policy in the nine border districts of West Bengal. Under the policy, farmers in these districts would stop wheat cultivation for at least two years. The present scoping study assesses the potential economic feasibility of alternative crops to wheat. Of the ten crops considered, maize, gram (chickpea), urad (black gram), rapeseed and mustard, and potatoes are found to be potentially feasible alternative crops. Any crop substitution would need support to ease the transition including addressing the challenges related to the management of alternative crops, ensuring adequate crop combinations and value chain development. Still, as wheat is a major staple, there is some urgency to support further research on disease epidemiology and forecasting, as well as the development and dissemination of blast-resistant wheat varieties across South Asia.

show abstract

Cautionary Notes on Use of the MoT3 Diagnostic Assay for Magnaporthe oryzae Wheat and Rice Blast Isolates

Cited by 28 publications

References 13 publications

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

Wheat blast: a new threat to food security

Averting wheat blast by implementing a ‘wheat holiday’: In search of alternative crops in West Bengal, India

Contact Info

Product

Resources

About