Caveolae at a glance

Bastiani, Michele; Parton, Robert G.

doi:10.1242/jcs.070102

Cited by 186 publications

(165 citation statements)

References 96 publications

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“…Yet, their formation generally follows the same basic principle, involving the assembly of peripheral or integral membrane proteins into a molecular scaffold that influences the local composition of the bilayer by attracting some additional proteins and lipids while excluding others. The biogenesis of caveolae, for instance, relies on the incorporation of caveolins and cavins into multimeric membrane-embedded protein complexes that create flask-shaped invaginations of the plasma membrane enriched in sphingolipids and cholesterol (Bastiani and Parton , 2010 ). Caveolae have been implicated in a wide variety of cellular processes, including fatty acid uptake, endocytosis, cell signaling, and mechanosensing.…”

Section: Microcompartments Within Membranesmentioning

confidence: 99%

Cellular microcompartments constitute general suborganellar functional units in cells

Holthuis

Ungermann

2013

Biological Chemistry

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All cells are compartmentalized to facilitate enzymatic reactions or cellular dynamics. In eukaryotic cells, organelles differ in their protein/lipid repertoire, luminal ion composition, pH, and redox status. In addition, organelles contain specialized subcompartments even within the same membrane or within its lumen. Moreover, the bacterial plasma membrane reveals a remarkable degree of organization, which is recapitulated in eukaryotic cells and often linked to cell signaling. Finally, protein-based compartments are also known in the bacterial and eukaryotic cytosol. As the organizing principle of such cellular subcompartments is likely similar, previous definitions like rafts, microdomains, and all kinds of ' -somes ' fall short as a general denominator to describe such suborganellar structures. Within this review, we will introduce the term cellular microcompartment as a general suborganellar functional unit and discuss its relevance to understand subcellular organization and function.

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Section: Microcompartments Within Membranesmentioning

confidence: 99%

Cellular microcompartments constitute general suborganellar functional units in cells

Holthuis

Ungermann

2013

Biological Chemistry

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“…Recently, major advances have been made in our understanding of caveolae integrity and generation through the identification of the cavin coat proteins and regulatory proteins integral to caveolae. In mammalian cells, four different cavins are expressed (cavin1, cavin2, cavin3 and cavin4, also known as PTRF, SDPR, PRKCDBP and MURC, respectively), which form distinct complexes in the cytosol and associate with caveolin to build up the caveolae coat (Bastiani and Parton, 2010;Shvets et al, 2014). Cavin4 is, similar to the caveolin1 homologue caveolin3, specific to muscle cells (Bastiani et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae

Mohan

Morén

Larsson

et al. 2015

Journal of Cell Science

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Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis owing to their dynamic behaviour ranging from stable surface association to dynamic rounds of fission and fusion with the plasma membrane. The caveolae coat is generated by oligomerisation of the membrane protein caveolin and the family of cavin proteins. Here, we show that cavin3 (also known as PRKCDBP) is targeted to caveolae by cavin1 (also known as PTRF) where it interacts with the scaffolding domain of caveolin1 and promote caveolae dynamics. We found that the N-terminal region of cavin3 binds a trimer of the cavin1 N-terminus in competition with a homologous cavin2 (also known as SDPR) region, showing that the cavins form distinct subcomplexes through their N-terminal regions. Our data shows that cavin3 is enriched at deeply invaginated caveolae and that loss of cavin3 in cells results in an increase of stable caveolae and a decrease of caveolae that are only present at the membrane for a short time. We propose that cavin3 is recruited to the caveolae coat by cavin1 to interact with caveolin1 and regulate the duration time of caveolae at the plasma membrane.

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“…The functional roles of caveolae are diverse and tissue-specific and include cellular processes such as lipid and glucose homeostasis and endocytosis (Capozza et al, 2005;Razani et al, 2002). Caveolae are moreover considered to play an important but complex role in cellular signalling through their ability to sequester signalling molecules (Bastiani and Parton, 2010).…”

Section: Introductionmentioning

confidence: 99%