CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis

Sardar, Atish; Nandi, Ashis Kumar; Chattopadhyay, Debasis

doi:10.1093/jxb/erx170

Cited by 58 publications

(33 citation statements)

References 66 publications

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“…Ca 2+ signalling has a regulatory effect on HR induced by P. triticina infection (Guan et al , 2006), and an extracellular calcium influx to the cell was shown to be a major source of calcium signalling (Liu et al , 2010). Plants overexpressing CBL‐interacting protein kinase 6 negatively regulate immunity and were more susceptible to Pseudomonas syringae in Arabidopsis (Sardar et al , 2017). Wheat calcium‐dependent protein kinase TaCPK7‐D overexpression activates defence‐related genes, leading to enhanced resistance to sharp eyespot caused by Rhizoctonia cerealis , and positively regulates host resistance to sharp eyespot disease (Wei et al , 2016).…”

Section: Discussionmentioning

confidence: 99%

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

Sun

Liu

et al. 2020

Molecular Plant Pathology

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractLeaf rust, caused by Puccinia triticina, is one of the most destructive fungal diseases in wheat production worldwide. The hypersensitive reaction (HR) is an important defence response against P. triticina infection. In this study, the physiological races 165 and 260 of P. triticina were combined with a line derived from the bread wheat cultivar Thatcher with the leaf rust resistance locus Lr26 to form compatible and incompatible combinations, respectively. Based on an RNA-Seq database of the interaction systems, a new wheat cysteine-rich receptor-like kinase gene, TaCRK2, is specifically induced and up-regulated in the incompatible combination. We identified that TaCRK2 was regulated in a Ca 2+ -dependent manner. Knockdown of TaCRK2 by virus-induced gene silencing and RNAi leads to a dramatic increase in HR area and the number of haustorial mother cells at the single infection site. In addition, urediniospores, a P. triticina-specific pathogenic marker in compatible combinations, were observed on leaf surfaces of silenced plants at approximately 15 days after inoculation in the incompatible combination. Moreover, transcription levels of TaPR1, TaPR2, and TaPR5 were obviously reduced in TaCRK2-silenced plants. TaCRK2 overexpression in Nicotiana benthamiana induced strong HR-like cell death. Finally, transient expression of green fluorescent protein fused with TaCRK2 in N. benthamiana indicated that TaCRK2 localizes in the endoplasmic reticulum. Thus, TaCRK2 plays an important role in the resistance to P. triticina infection and has a positive regulation effect on the HR cell death process induced by P. triticina. K E Y W O R D S cysteine-rich receptor-like protein kinase, hypersensitive reaction, Puccinia triticina, VIGS, wheat | 733 GU et al.

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Section: Discussionmentioning

confidence: 99%

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

Sun

Liu

et al. 2020

Molecular Plant Pathology

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“…Arabidopsis AtCIPK6 negatively regulates ROS production in ETI and PTI. Atcipk6 loss-of-function mutant plants possess more resistance to Pseudomonas syringae, but overexpression lines are more susceptible [170]. In rice, OsCIPK14 and the duplicated gene OsCIPK15 are rapidly induced by MAMPs, and their proteins interact with OsCBL4.…”

Section: The Cbl-cipk Network In Biotic Stressmentioning

confidence: 99%

The CBL–CIPK Pathway in Plant Response to Stress Signals

et al. 2020

IJMS

107

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Plants need to cope with multitudes of stimuli throughout their lifecycles in their complex environments. Calcium acts as a ubiquitous secondary messenger in response to numerous stresses and developmental processes in plants. The major Ca2+ sensors, calcineurin B-like proteins (CBLs), interact with CBL-interacting protein kinases (CIPKs) to form a CBL–CIPK signaling network, which functions as a key component in the regulation of multiple stimuli or signals in plants. In this review, we describe the conserved structure of CBLs and CIPKs, characterize the features of classification and localization, draw conclusions about the currently known mechanisms, with a focus on novel findings in response to multiple stresses, and summarize the physiological functions of the CBL–CIPK network. Moreover, based on the gradually clarified mechanisms of the CBL–CIPK complex, we discuss the present limitations and potential prospects for future research. These aspects may provide a deeper understanding and functional characterization of the CBL–CIPK pathway and other signaling pathways under different stresses, which could promote crop yield improvement via biotechnological intervention.

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“…In the majority of cases CBLs are interact with CIPKs (CBL-interacting protein kinases) and regulate diverse functions including abscisic acid signaling and salt tolerance and [61,62]. The CIPKs which are the major interacting partner of CBLs are involved in immune response, response to salt, osmotic and abscisic acid stress, plant growth and development [61,62,63]. Wheat CIPK 25 is negatively regulate salt tolerance in the transgenic wheat [64].…”

Section: Interactome Map Of Cams Cmls Cbls and Cdpksmentioning

confidence: 99%

Molecular Players of EF-hand Containing Calcium Signaling Event in Plants

Mohanta

Yadav

Khan

et al. 2019

IJMS

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Ca2+ is a universal second messenger that plays a pivotal role in diverse signaling mechanisms in almost all life forms. Since the evolution of life from an aquatic to a terrestrial environment, Ca2+ signaling systems have expanded and diversified enormously. Although there are several Ca2+ sensing molecules found in a cell, EF-hand containing proteins play a principal role in calcium signaling event in plants. The major EF-hand containing proteins are calmodulins (CaMs), calmodulin like proteins (CMLs), calcineurin B-like (CBL) and calcium dependent protein kinases (CDPKs/CPKs). CaMs and CPKs contain calcium binding conserved D-x-D motifs in their EF-hands (one motif in each EF-hand) whereas CMLs contain a D-x3-D motif in the first and second EF-hands that bind the calcium ion. Calcium signaling proteins form a complex interactome network with their target proteins. The CMLs are the most primitive calcium binding proteins. During the course of evolution, CMLs are evolved into CaMs and subsequently the CaMs appear to have merged with protein kinase molecules to give rise to calcium dependent protein kinases with distinct and multiple new functions. Ca2+ signaling molecules have evolved in a lineage specific manner with several of the calcium signaling genes being lost in the monocot lineage.

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CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis

Abstract: CBL-interacting protein kinase 6 (CIPK6) negatively regulates the PAMP-triggered and effector-triggered immune response in Arabidopsis.

Cited by 58 publications

References 66 publications

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

The CBL–CIPK Pathway in Plant Response to Stress Signals

Molecular Players of EF-hand Containing Calcium Signaling Event in Plants

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