CBX7 Inhibits Cell Growth and Motility and Induces Apoptosis in Cervical Cancer Cells

Li, Rong; Yan, Qi; Tian, Ping; Wang, Yan; Wang, Jing; Ning, Tao; Li, Ning; Xin, Lin; Ding, Liang; Liu, Jiwen; Ma, Cailing

doi:10.1016/j.omto.2019.09.002

Cited by 30 publications

(31 citation statements)

References 35 publications

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“…Accumulating evidence indicates that decreased CBX7 may also serve as an adverse prognostic biomarker for colon carcinoma patients [26]. The latest study reveals that CBX7 inhibits cell motility and cell growth and induces apoptosis in cervical cancer [27]. The present study showed that decreased CBX7 expression in CESC, this result is consistent with ndings for CBX7 expression in other types of tumors.…”

Section: Discussionsupporting

confidence: 90%

Decreased Expression of CBX7 is an Independent Predictor of Poor Survival in Cervical Cancer

An¹,

Zhang²,

Jin³

et al. 2021

Preprint

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Background: CBX7, a component of the PRC1, has been investigated as a potential biomarker in human malignant neoplasias. In present study, the value of CBX7 expression in the diagnostic and prognosis of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) as examined via bioinformatics analysis of data obtained from Genotype-Tissue Expression (GTEx) database and The Cancer Genome Atlas (TCGA) database.Methods: Relationships between clinical factors and CBX7 were explored. The Kaplan-Meier method and Cox regression were used to identify the associations between clinicopathological characteristics and overall survival (OS) in CESC. Gene set enrichment analysis (GSEA) was performed using TCGA dataset. Results: Our results indicated the decreased expression of CBX7 in CESC, and difference in CBX7 expression was also identified according to age subgroups. CESC patients with decreased CBX7 expression had worse prognosis than those with high CBX7 expression. Multivariate analysis showed that CBX7 was an independent risk factor for OS. GSEA demonstrated pathways involved in the biosynthesis of unsaturated fatty acids, glycosaminoglycan biosynthesis-chondroitin sulfate, glyoxylate and dicarboxylate metabolism, nod-like receptor signaling pathway, O-glycan biosynthesis, one carbon pool by folate and protein export as differentially enriched in CESC with decreased CBX7 expression.Conclusion: We demonstrated that decreased CBX7 expression may be a potential independent biomarker for poor prognosis in CESC.

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Section: Discussionsupporting

confidence: 90%

Decreased Expression of CBX7 is an Independent Predictor of Poor Survival in Cervical Cancer

An¹,

Zhang²,

Jin³

et al. 2021

Preprint

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“…In conclusion, Foxp3 is a risk factor for the survival of cervical cancer, which is consistent with our current findings. CBX7 inhibits the proliferation of cervical cancer cells [ 43 ]. LTA inhibits the proliferation of CD4(+) T-cells in a FoxP3(+) Treg-dependent manner in patients with chronic hepatitis C, suggesting that LTA acts on FoxP3 [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Immunogenomic Identification for Predicting the Prognosis of Cervical Cancer Patients

Wang

Vattai

Vilsmaier

et al. 2021

IJMS

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Cervical cancer is primarily caused by the infection of high-risk human papillomavirus (hrHPV). Moreover, tumor immune microenvironment plays a significant role in the tumorigenesis of cervical cancer. Therefore, it is necessary to comprehensively identify predictive biomarkers from immunogenomics associated with cervical cancer prognosis. The Cancer Genome Atlas (TCGA) public database has stored abundant sequencing or microarray data, and clinical data, offering a feasible and reliable approach for this study. In the present study, gene profile and clinical data were downloaded from TCGA, and the Immunology Database and Analysis Portal (ImmPort) database. Wilcoxon-test was used to compare the difference in gene expression. Univariate analysis was adopted to identify immune-related genes (IRGs) and transcription factors (TFs) correlated with survival. A prognostic prediction model was established by multivariate cox analysis. The regulatory network was constructed and visualized by correlation analysis and Cytoscape, respectively. Gene functional enrichment analysis was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). A total of 204 differentially expressed IRGs were identified, and 22 of them were significantly associated with the survival of cervical cancer. These 22 IRGs were actively involved in the JAK-STAT pathway. A prognostic model based on 10 IRGs (APOD, TFRC, GRN, CSK, HDAC1, NFATC4, BMP6, IL17RD, IL3RA, and LEPR) performed moderately and steadily in squamous cell carcinoma (SCC) patients with FIGO stage I, regardless of the age and grade. Taken together, a risk score model consisting of 10 novel genes capable of predicting survival in SCC patients was identified. Moreover, the regulatory network of IRGs associated with survival (SIRGs) and their TFs provided potential molecular targets.

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“…Cells were incubated in six-well plates overnight and transfected with miR-506 mimics, Skp2 cDNA, Skp2 siRNA or a combination for 72 hours. Then the cells were collected and apoptosis was analyzed [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…Treated cells were centrifuged and lysed by RIPA lysis buffer, and proteins in cell lysates were subjected to SDS-PAGE and further transferred to PVDF membrane. The western blotting was done as described before [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

MiR-506 exerts antineoplastic effects on osteosarcoma cells via inhibition of the Skp2 oncoprotein

Ding

Sun

Yan

et al. 2021

Aging

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S-phase kinase-associated protein 2 (Skp2) performs oncogenic functions in cancers; however, how Skp2 is regulated post-transcriptionally is elusive in osteosarcoma. Therefore, we determined whether miR-506 could directly target Skp2 in osteosarcoma to perform its tumor suppressive functions. Here, we found that miR-506 mimics suppressed cell viability, induced apoptosis, and attenuated migration and invasion in osteosarcoma cells. Moreover, upregulation of Skp2 accelerated cell viability and motility and rescued the tumor suppressive effect of miR-506 in osteosarcoma cells. Moreover, downregulation of Skp2 inhibited cell viability and decreased cell motility, which enhanced the antitumor activity induced by miR-506 mimic transfection in osteosarcoma cells. Our western blotting results implied that miR-506 inhibited Skp2 expression and subsequently upregulated Foxo1 and p57 in OS cells. In summary, miR-506 performs an anticancer activity via directly targeting Skp2 in osteosarcoma cells, indicating that inactivation of Skp2 by miR-506 might be an alternative strategy for treating osteosarcoma.

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CBX7 Inhibits Cell Growth and Motility and Induces Apoptosis in Cervical Cancer Cells

Cited by 30 publications

References 35 publications

Decreased Expression of CBX7 is an Independent Predictor of Poor Survival in Cervical Cancer

Decreased Expression of CBX7 is an Independent Predictor of Poor Survival in Cervical Cancer

Immunogenomic Identification for Predicting the Prognosis of Cervical Cancer Patients

MiR-506 exerts antineoplastic effects on osteosarcoma cells via inhibition of the Skp2 oncoprotein

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