CCK reduces the food intake mainly through CCK1R in Siberian sturgeon (Acipenser baerii Brandt)

Zhang, Xin; Tang, Ni; Qi, Jinwen; Wang, Shuyao; Hao, Jin; Wu, Yingliang; Chen, Hu; Tian, Zhengzhi; Wang, Bin; Chen, Defang; Li, Zhiqiong

doi:10.1038/s41598-017-12646-3

Cited by 35 publications

(27 citation statements)

References 49 publications

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“…The CCKA receptor (CCKAR) has a greater affinity for sulfated than non-sulfated CCK whereas the binding ability of the B receptor (CCKBR) is not affected by sulfation of the tyrosine residue in CCK-8 (Dufresne et al, 2006; Staljanssens et al, 2011). Since the structure of CCK-8 is highly conserved through phylogeny, including fish (Johnsen, 1998; Murashita et al, 2006; Zhang et al, 2017), the commercial sulfated CCK has been used to study the function CCK peptides in appetite regulation and gastrointestinal motility (Olsson et al, 1999; Forgan and Forster, 2007; Tinoco et al, 2015; Zhang et al, 2017). Also, the wrasse CCK-8 has seven out of eight amino acids that are similar to those in human CCK-8 (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

“…In ballan wrasse, we identified five CCK-receptor genes corresponding to CCKAR and CCKBR in other teleosts. CCKAR has been reported to be involved in contractile response of fish intestine (Tinoco et al, 2015; Zhang et al, 2017). The CCKAR has been known to be more involved in digestion than the CCKBR in mammals (Thomas et al, 1979; Dufresne et al, 2006; Staljanssens et al, 2011; Rehfeld, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…However, a full understanding of the CCK receptors role in gastrointestinal functions is still lacking. Only two reports describe the function of CCK receptors, one in goldfish (Tinoco et al, 2015) and one for Siberian sturgeon (Zhang et al, 2017). These studies showed a contribution of CCKAR in the intestinal contractile response, while the CCKBR showed less involvement in digestive function.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

Lie

Giroud-Argoud

et al. 2019

Front. Neurosci.

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Cholecystokinin (CCK) is well-known as a key hormone that inhibits stomach emptying and stimulates midgut motility in gastric species. However, the function of CCK related to gut motility in agastric fish, especially in fish with a short digestive tract such as ballan wrasse, remains unknown. Here we present a detailed description of the spatio-temporal quantification of intestinal motility activity in vitro comprising the complete intestinal tract in ballan wrasse. We show that CCK modulates intestinal motility, having multiple effects on motility patterns depending on location in the gut and types of contractions. CCK reduced propagating contractions in the foregut, but it increased both non-propagating and propagating contractions in the hindgut. CCK also altered the direction of propagating contractions, as it reduced anterograde ripples and slow propagating contractions. The velocity of propagating contractions was slowed down by CCK. CCK also reduced the amplitude of standing contractions and ripples, but it did not alter the amplitude of slow propagating contractions. The presence of CCKA receptor antagonist modulated the motility responses of ballan wrasse intestines when exposed to CCK. We also showed that CCK reduced the intestinal length and stimulated motility to empty the gallbladder. Based on our findings we hypothesize that CCK, mainly through the CCKA receptor, modulates non-propagating and propagating contractions to optimize digestion and absorption and regulate the intestinal evacuation in ballan wrasse. We also found evidence that the modulation of intestinal motility by CCK is different in agastric fish from that in gastric vertebrates. We suggest that this is an evolutionary adaptation to optimize digestion without a stomach.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

Lie

Giroud-Argoud

et al. 2019

Front. Neurosci.

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“…According to these authors, s the administration of Cholecystokinin A (CCKA) receptor antagonists suppressed the effect on satiety. These receptors inhibited the anorect ic effect of exogenous cholecystokinin in rats and mice [36,37]. The increase in satiety caused by simmondsin and that of CCK is generally due to the vague nerve.…”

Section: Discussionmentioning

confidence: 99%

Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Belhadj

Dal

Fakhreddine³

et al. 2020

Nutr Metab (Lond)

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Background: Evaluation of the action of various traditional plants to treat metabolic syndrome are strongly studied. In our study, we investigated the effect of the Tunisian jojoba seed on a metabolic syndrome induced in rat by the High Fat diet and High Fructose (HFHF) and its renal and hepatic complications. Methods: The rats were fed with HFHF or Normal Diet (ND) for a period of 8 weeks. After that, a switch from HFHF to ND or Normal Diet Jojoba (NDJ),(jojoba diet approach) or High Fat and High Fructose and Jojoba diet (HFHFJ) (nutraceutical approach) has been done. Metabolic disorder was evaluated by measuring the fasting body weight, glycemia and C-peptide and leptin. Oxidative stress parameters like ThioBarbituric Acid Reactive Substances (TBARS) and Total Antioxidant Capacity (TAOC) were analyzed in the plasma and renal and hepatic function were determined by the measure of creatinine and alanine transferase (ALT) respectively. Histological analysis was performed on the liver, kidney and pancreas. Results: HFHF diet exhibited characteristics of metabolic syndrome presented by insulin resistance, hyperinsulinemia, hyperleptinemia, fat mass with hepatic steatosis and renal disorder. HFHF diet was associated with oxidative stress (OS) presented by an increase in TBARS and a decrease in TAOC. Adding jojoba seeds to HFHF rat group diet induced a decrease in body weight, fat mass (58 and 41%), insulin resistance (59 and 56%), oxidative stress (60 and 41%), liver steatosis (from a score = 3 to a score = 0) and renal complications (25 and 42%). This effect was emphasized with diet approach. Conclusion: The results demonstrated the beneficial effect of jojoba against metabolic syndrome and oxidative stress, suggesting that jojoba could be used in the prevention and treatment of metabolic syndrome.

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“…Cholecystokinin (CCK), a key hormone in the control of digestion, is released when food is present in the gut in both mammals (e.g., Grider, 1994; Liou et al, 2011; McLaughlin et al, 1998) and fish (e.g., MacDonald and Volkoff, 2009; Pitts and Volkoff, 2017). CCK performs its functions related to gastrointestinal motility and digestion mainly through Cholecystokinin A Receptor (CCKAR) in mammals [reviewed by Staljanssens et al (2011)] and in fish (Tinoco et al, 2015; Zhang et al, 2017). We have previously found that in ballan wrasse, CCK works mainly through CCKA receptors to suppress propulsive contractions, thus prolonging the residence of food in the bulbous for optimal digestion (Le et al, 2019a).…”

Section: Discussionmentioning

confidence: 99%

Nutrient sensing; transcriptomic response and regulation of gut motility in an agastric vertebrate

Lie

Etayo

et al. 2019

Preprint

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7 93 juveniles as a model. This fish does not have a stomach and the whole intestine was used for the 94 study. Differences in gene expression were compared between intestines administered cellulose 95 versus empty intestines in order to isolate the effect of mechanical sensing; and intestines 96 administered with either protein or lipid versus cellulose to compare effects of chemical sensing on 97 enterocyte metabolism. We also described postprandial motility patterns as responses to the 98 administration of the compounds in the ballan wrasse intestines. 99 2. Materials and Methods 100 2.1. Nutrient bolus 101 Six different diets, including intact lipid (IL), hydrolyzed lipid (HL), intact protein (IP), hydrolyzed 102 protein (HP), cellulose (CL) and plastic beads (PB) were prepared. Intact lipid was made by stirring a 103 mix of 80% (by volume) cod liver oil (Møllers Tran, containing omega-3-fatty acids and Vitamin D), 104 15% phosphate-buffered saline (PBS) pH = 8, and 5% Tween 20 (TWEEN® 20, P9416 Sigma Aldrich) to 105 obtain the same viscosity as the hydrolyzed lipid diet. To make 5 mL hydrolyzed lipid diet, we 106 incubated 4 mL cod liver oil with 3.5 mg lipase (Lipase from Pseudomonas cepacia -62309 Sigma Aldrich, 107 ≥30 U/mg) dissolved in 750 µL PBS (pH = 8) at 40 o C for 5 hours. The pH of the mix was maintained at 108 around 8 by titrating with 5 M NaOH during incubation. The mix was then incubated at 80 o C for 2 109 hours to deactivate the lipase before mixing with 250 µL Tween. 20. Five mL of intact protein diet was 110 prepared by combining 2 g casein with 4 mL 100 mM NH4HCO3 and 1 mL 1 mM HCl. Hydrolyzed 111 protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, 112 Porcine 1:250, 85450C, Sigma Aldrich) at 37 o C for 20 hours, followed by 80 o C for 2 hours for enzyme 113 deactivation. 5 mL of hydrolyzed protein diet was freshly mixed with 100 µL protease inhibitor 114 [Protease/Phosphatase Inhibitor Cocktail (100X) #5872, Cell Signaling Technology, Inc, The 115 Netherlands] before administration of the bolus into the intestine. Cellulose diet was created using 0.5 116 mg cellulose (Cellulose microcrystalline powder, 435236 Sigma Aldrich) in 1.5 mL H2O, adding a tiny 117 amount of Brillant Blue (Brilliant Blue R, B7920 Sigma-Aldrich) as a color maker. These five bolus types 118 were made and stored at -20 o C for use within a week. The diets were thawed and warmed up at 14 o C

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CCK reduces the food intake mainly through CCK1R in Siberian sturgeon (Acipenser baerii Brandt)

Cited by 35 publications

References 49 publications

Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

Effects of Cholecystokinin (CCK) on Gut Motility in the Stomachless Fish Ballan Wrasse (Labrus bergylta)

Anorexic and metabolic effect of jojoba: potential treatment against metabolic syndrome and hepatic complications

Nutrient sensing; transcriptomic response and regulation of gut motility in an agastric vertebrate

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