CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Sipert, Carla Renata; Morandini, Ana Carolina; Módena, Karin Cristina da Silva; Dionísio, Thiago José; Machado, Maria Aparecida de Andrade Moreira; Oliveira, Sandra Helena Penha de; Campanelli, Ana Paula; Santos, Carlos

doi:10.1590/1678-7757201300004

Cited by 25 publications

(45 citation statements)

References 39 publications

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“…Barrett's esophagus (BAR-T cell line) were stimulated with the TLR4 agonist lipopolysaccharide (LPS).It was shown that stimulation of TLR4 with LPS resulted in NF-κB activation and an increase of IL-8 secretion (Verbeek et al 2014). In fact, the structure of the LPS of Porphyromonasgingivalis (PgLPS) has a further particular antigenic moiety and is recognized by TLR2, which is responsible for the recognition of cell wall components of gram positive bacteria (Sipert et al 2013). Genetic studies have been performed on TLR polymorphisms in esophageal cancer.…”

Section: Resultsmentioning

confidence: 99%

Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines

Gonçalves

Cappellari

Santos

et al. 2016

Braz. arch. biol. technol.

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The esophagus and mouth tumors are very frequent malignancies worldwide. Lipopolysaccharides (LPS) are capable of regulating gene expression of pro-inflammatory cytokines by binding to toll-like receptor 4 (TLR4). Recent studies show that LPS can increase the migration ability of human esophageal cancer cell line HKESC-2 by increasing its adhesion properties. However, the effect of LPS has not been tested on viability of human esophageal and oral cancer cells. This study aimed to determine the action of LPS on the cell proliferation and viability in

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Section: Resultsmentioning

confidence: 99%

Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines

Gonçalves

Cappellari

Santos

et al. 2016

Braz. arch. biol. technol.

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“…Teeth and gingival tissues were donated under patient informed consent (third molars from three adults aged 18–26, canines from three children aged 7–9). Human fibroblasts from gingiva (GF) (n=3), periodontal ligament (PLF) (n=3), dental pulp from permanent (PDPF) (n=3) and deciduous (DDPF) (n=3) teeth were cultured using an explant technique as described previously (6,7,9,23). Tissues were fragmented and individually incubated for cell growth in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Life Technologies Corporation, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, Invitrogen) and antibiotics (100 μg/mL penicillin, 100 μg/mL streptomycin, 0.5 mg/mL amphotericin B – Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

“…Cultured cells were characterized as fibroblasts by their morphology and positivity for Fibroblast Specific Protein-1 (FSP-1) (9,24). Immunostaining was performed using mouse anti-human FSP-1 (1B10) (Abcam, Cambridge, UK) followed by fluorescein conjugated anti-mouse IgG (Abcam).…”

Section: Methodsmentioning

confidence: 99%

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In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts

Sipert

Morandini

Dionísio

et al. 2014

Journal of Endodontics

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Introduction Production of chemokines by tissue resident cells is one of the main mechanisms involved in the inflammatory infiltrate formation during inflammation. The specific ability of fibroblasts from different oral tissues such as gingiva, periodontal ligament and dental pulp from permanent and deciduous teeth in producing the chemokines CCL3 and CXCL12 under stimulation by bacterial products commonly found in endodontic infections was investigated. Methods Cultures of fibroblasts from gingiva and periodontal ligament as well as from dental pulp from permanent and deciduous teeth were established by using an explant technique and stimulated with increasing concentrations of Escherichia coli lipopolysaccharide (EcLPS) and Enterococcus faecalis lipoteichoic acid (EfLTA) for 1, 6 and 24 hours. Supernatants were tested for CCL3 and CXCL12 by enzyme-linked immunosorbent assay. Results In general, CCL3 production was induced by EcLPS in the 4 fibroblast subtypes and by EfLTA in fibroblasts from gingiva and periodontal ligament. Constitutive CXCL12 synthesis decreased in all fibroblast subtypes especially under stimulation with EcLPS. Fibroblast from permanent deciduous teeth was the cell type presenting the most expressive reduction in CXCL12 release by both stimuli. On the basis of computational matching of CXCL12 mRNA with the microRNAs miR-141 and miR-200a, their expression was also investigated. Although detected in the fibroblasts, these molecules remained unaltered by bacterial by-product stimulation. Conclusions EcLPS and EfLTA induced the production of CCL3 and unbalanced the synthesis of CXCL12 in a manner dependent on the specific tissue origin of fibroblasts.

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“…However, it has been clearly demonstrated that the biological behavior of fibroblasts is related to the type of connective tissue from which they originate (2)(3)(4). Such properties include membrane receptor expression (3) and cytokine release kinetics (2,3,(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro

et al. 2014

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MicroRNAs (miRNAs) are small noncoding RNAs showing a tissue-specific expression pattern, and whose function is to suppress protein synthesis. In this study, we hypothesized that expression of miRNAs would differ among fibroblasts from dental pulp (DPF), gingiva (GF) and periodontal ligament (PLF) in vitro. Once established by an explant technique, DPF, GF and PLF were collected for RNA isolation and subjected to a miRNA microarray. Next, cells were stimulated with E. coli lipopolysaccharide (LPS) for 24 h and then collected for RNA isolation. Expression of miR-146a and miR-155 was investigated by qPCR. Microarray screening revealed several miRNAs that showed specifically high expression in at least one of the fibroblast subtypes. These molecules are potentially involved in the regulation of extracellular matrix turnover and production of inflammatory mediators. Microarray analysis showed that both miR-146a and miR-155 were among the miRNAs expressed exclusively in GF. qPCR demonstrated significant upregulation of miR-146a only in GF after LPS stimulation, whereas basal expression of miR-155 was higher in GF than in the other cell subtypes. LPS downregulated the expression of miR-155 only in GF. Our results suggest that the expression and regulation of miR-146a and miR-155 are more pronounced in GF than in DPF and PLF. (J Oral Sci 56, 157-164, 2014)

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CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS

Cited by 25 publications

References 39 publications

Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines

Effect of LPS on the Viability and Proliferation of Human Oral and Esophageal Cancer Cell Lines

In Vitro Regulation of CCL3 and CXCL12 by Bacterial By-products Is Dependent on Site of Origin of Human Oral Fibroblasts

MicroRNA-146a and microRNA-155 show tissue-dependent expression in dental pulp, gingival and periodontal ligament fibroblasts in vitro

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