CCNB2, NUSAP1 and TK1 are associated with the prognosis and progression of hepatocellular carcinoma, as revealed by co‑expression analysis

Abstract

The mortality rate associated with hepatocellular carcinoma (HCC) is the third highest among all digestive system tumors. However, the causes of HCC development and the underlying mechanisms have remained to be fully elucidated. In the present bioinformatics study, genetic markers were identified and their association with HCC was determined. The mRNA expression datasets GSE87630, GSE74656 and GSE76427 were downloaded from the Gene Expression Omnibus (GEO) database. A total of 96 differentially expressed genes (DEGs) were screened from the 3 GEO datasets, including 25 upregulated and 71 downregulated genes. DEGs were uploaded to the database for Annotation, Visualization and Integrated Discovery to screen for enriched Gene Ontology terms in various categories and the Search Tool for the Retrieval of Interacting Genes/Proteins was used to identify the interactions and functions of the DEGs. A total of 3 genetic markers were identified in a stepwise pathway and functional analysis in a previous study. The association of the genetic markers with prognosis was analysed using the UALCAN online analysis tool. Regression analysis was also performed to identify the relationship between HCC grade and disease recurrence and the expression of genetic markers using The Cancer Genome Atlas HCC dataset. In addition, the expression of the 3 genetic markers in HCC tissues was determined using reverse transcription-quantitative PCR, the Oncomine database and the Human Protein Atlas database. The expression levels of the 3 genetic markers cyclin B2 (CCNB2), nucleolar and spindle-associated protein 1 (NUSAP1) and thymidine kinase 1 (TK1) were significantly correlated with each other and high mRNA expression of CCNB2 was significantly associated with poor overall survival of patients with HCC. Receiver operating characteristic curve analysis indicated that NUSAP1 and TK1 were capable of distinguishing between recurrent and non-recurrent HCC. Furthermore, CCNB2, NUSAP1 and TK1 were highly correlated with the HCC grade. It was also indicated that the mRNA expression of CCNB2, NUSAPA and TK1 was increased in primary HCC tissues when compared with that in adjacent tissues. The present study identified that the CCNB2, NUSAP1 and TK1 genes may serve as prognostic markers for HCC, and may be of value from the perspectives of basic research and clinical treatment of HCC.

Identification of the hub gene BUB1B in hepatocellular carcinoma via bioinformatic analysis and in vitro experiments

“…Except for BUB1B, the mechanisms of the other hub genes in hepatocarcinogenesis have been elucidated to varying degrees ( Foijer et al, 2017 ; Jin et al, 2020 ; Li et al, 2014 ; Li, Bai & Zhang, 2017 ; Liu et al, 2020 ; Wu et al, 2018b ; Yang, Lin & Liu, 2020 ). Therefore, the relationship between BUB1B and other hub genes was investigated first.…”

“…CCNB1, CCNB2, CDK1 and CDC20, are all cell cycle-related proteins. Many studies have demonstrated their oncogenic roles in the regulation of cell cycle progression in a series of tumors, such as hepatocellular carcinoma ( Jin et al, 2020 ; Li et al, 2014 ; Liu et al, 2020 ; Wu et al, 2018a ), bladder cancer ( Heo et al, 2020 ; Kim et al, 2014 ), non-small lung cancer ( Qian et al, 2015 ; Wang et al, 2019a ), pancreatic cancer ( Wei et al, 2013 ; Zhang et al, 2018 ) and breast cancer ( Kidokoro et al, 2008 ; Kim et al, 2012 ; Parmar et al, 2018 ).…”

CCNB2 as a potential biomarker of bladder cancer via the high throughput technology

“…Abnormal expression of CCNB2 malregulates spindle checkpoints in the cell cycle and leads to chromosomal instability, one of the signature phenotypes of most cancers. [20][21][22] CCNB2 regulates the activity of cyclin-dependent kinases. It is well known that different cyclins can regulate the eukaryotic cell cycle through CDK at specific points in the mitotic cycle.…”

“…Abnormal expression of CCNB2 malregulates spindle checkpoints in the cell cycle and leads to chromosomal instability, one of the signature phenotypes of most cancers. [20–22]…”

NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in Prostate Cancer

“…Increased expression of nucleolar and spindle-associated protein 1 (NUSAP1) has been identified as a robust clinical biomarker of aggressive prostate cancer and is part of two commercial prognostic gene expression panels used to analyze prostate tissue samples [ 2 , 3 , 4 , 5 ]. Over the past decade, increased expression of NUSAP1 has been shown to be associated with poor outcomes in many solid tumors, including those of the skin [ 6 , 7 ], breast [ 8 , 9 , 10 , 11 ], prostate [ 2 , 3 , 12 ], cervix [ 13 , 14 ], brain [ 15 , 16 , 17 ], liver [ 18 , 19 , 20 ], pancreas [ 21 , 22 ], stomach [ 23 , 24 ], ovary [ 25 , 26 ] and lung [ 27 , 28 ].…”