CCTδ colocalizes with actin and β-tubulin: Insight into its involvement in the cytoskeleton formation of the intracellular parasite Nosema bombycis

Qi, Jingru; Zhu, Feng; Shao, Li; Chen, Ying; Li, Jicheng; He, Ping; Shang, Ruisha; Sun, Fuzhen; Wang, Qiang; Zhang, Shujun; Tang, Xudong; Shen, Zhongyuan

doi:10.1016/j.jip.2021.107646

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…RBC-EV miR-204 decreased cell viability, mitigated cell migration, and suppressed spheroid formation and growth, more strongly against MYCN-amp NB cells, but these anti-neuroblastoma effects were also observed, but to a lesser extent, in MYCN-NA cells (Figures 5-7). These results are consistent with previous evidence, in which miR-204 exhibited anticancer activities against several cancers with diversed oncogenic backgrounds, i.e., oral squamous cell carcinoma (OSCC), renal cell carcinoma, and non-small-cell lung cancer [47][48][49][50]. As RBC-EV miR-204 could inhibit both NB cell migration and spheroid formation and growth, the use of RBC-EV miR-204 as an adjuvant therapy may mitigate metastasis in high-risk neuroblastoma, regardless MYCN amplification status.…”

Section: Discussionsupporting

confidence: 93%

Red blood cell-derived extracellular vesicles with miR-204 mimic loading for pediatric neuroblastoma treatment

Chiangjong

Panachan

Keadsanti

et al. 2023

Preprint

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Neuroblastoma (NB) is the most common extracranial solid tumor in pediatric population with a high degree of heterogeneity in clinical outcomes, ranging from spontaneous remission to rapid progression and death. Upregulation of a tumor suppressor miR-204 in patient-derived neuroblastoma tumors was associated with good prognosis independent of known risk factors. While miR-204 is recognized as a therapeutic candidate, its delivery was unavailable. This study aimed to develop red blood cell-derived extracellular vesicles (RBC-EVs) as the miR-204 carrier and evaluate the inhibitory activity against neuroblastoma cell lines and spheroids. MiR-204 mimics were loaded into RBC-EVs (RBC-EVmiR-204) by electroporation with the optimized parameters of 250 V, 20 ms, 10 pulsing times. RBC-EVmiR-204, but not the native RBC-EVs, could inhibit cell viability, migration and spheroid formation and growth of MYCN-amp and MYCN non-amplification (MYCN-NA) NB cells, even though the suppressive effects were more preferable in MYCN-amp NB. For the mechanistic insight, SWATH-proteomics suggested that RBC-EVmiR-204induced dysregulation of ribosomal proteins and alterations in RNA metabolism, leading to inhibiting neuroblastoma progression. This study developed RBC-EVmiR-204as an alternative/adjunct therapy of pediatric neuroblastoma. The therapeutic efficacy of RBC-EVmiR-204should be further investigated in preclinical models and clinical studies.

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Section: Discussionsupporting

confidence: 93%

Red blood cell-derived extracellular vesicles with miR-204 mimic loading for pediatric neuroblastoma treatment

Chiangjong

Panachan

Keadsanti

et al. 2023

Preprint

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“…The transcriptional level of NbCCTα was downregulated after the knockdown of the NbCCTζ gene at 24 h, 72 h, and 96 h, whereas it was significantly upregulated at 48 h; moreover, the proliferation of N. bombycis was significantly inhibited, indicating that the NbCCTζplays a crucial role in the proliferation of N. bombycis . Our previous studies have revealed that NbCCTα is related to cell division, and NbCCTδ plays an efficient role in keeping the integrity of the cytoskeleton [ 24 , 37 ]. Combining the results of this study with those of our previous work, we summarize our knowledge of the expression pattern and subcellular localization of CCTζ, CCTδ, and CCTα in N. bombycis in Table S1 , which may help to perform further research on the function and relationship of CCT subunits in microsporidia.…”

Section: Discussionmentioning

confidence: 99%

The Function of Different Subunits of the Molecular Chaperone CCT in the Microsporidium Nosema bombycis: NbCCTζ Interacts with NbCCTα

Xu,

Chen,

et al. 2024

JoF

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Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone protein that consists of eight completely different subunits and assists in the folding of newly synthesized peptides. The zeta subunit of CCT is a regulatory factor for the folding and assembly of cytoskeletal proteins as individuals or complexes. In this study, the zeta subunit of Nosema bombycis (NbCCTζ) is identified for the first time. The complete ORF of the NbCCTζ gene is 1533 bp in length and encodes a 510 amino acid polypeptide. IFA results indicate that NbCCTζ is colocalized with actin and β-tubulin in the cytoplasm during the proliferative phase and that NbCCTζ is completely colocalized with NbCCTα in the cytoplasm of N. bombycis throughout the entire life cycle. Furthermore, the yeast two-hybrid assay revealed that the NbCCTζ interacts with NbCCTα. The transcriptional level of NbCCTζ is significantly downregulated by knocking down the NbCCTα gene, while the transcriptional level of NbCCTα is downregulated after knocking down the NbCCTζ gene. These results suggest that NbCCTζ may play a vital role in the proliferation of N. bombycis by coordinating with NbCCTα.

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“…The immunolocalization was observed under a fluorescence inverted microscope (Olympus IX-71). The detailed steps of immunolocalization referred to the method of Qi et al (2021) .…”

Section: Methodsmentioning

confidence: 99%

Identification and subcellular localization analysis of membrane protein Ycf 1 in the microsporidian Nosema bombycis

Chen

Wei

Chen

et al. 2022

PeerJ

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Microsporidia are obligate intracellular parasites that can infect a wide range of vertebrates and invertebrates including humans and insects, such as silkworm and bees. The microsporidium Nosema bombycis can cause pebrine in Bombyx mori, which is the most destructive disease in the sericulture industry. Although membrane proteins are involved in a wide range of cellular functions and part of many important metabolic pathways, there are rare reports about the membrane proteins of microsporidia up to now. We screened a putative membrane protein Ycf 1 from the midgut transcriptome of the N. bombycis-infected silkworm. Gene cloning and bioinformatics analysis showed that the Ycf 1 gene contains a complete open reading frame (ORF) of 969 bp in length encoding a 322 amino acid polypeptide that has one signal peptide and one transmembrane domain. Indirect immunofluorescence results showed that Ycf 1 protein is distributed on the plasma membrane. Expression pattern analysis showed that the Ycf 1 gene expressed in all developmental stages of N. bombycis. Knockdown of the Ycf 1 gene by RNAi effectively inhibited the proliferation of N. bombycis. These results indicated that Ycf 1 is a membrane protein and plays an important role in the life cycle of N. bombycis.

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CCTδ colocalizes with actin and β-tubulin: Insight into its involvement in the cytoskeleton formation of the intracellular parasite Nosema bombycis

Cited by 7 publications

References 28 publications

Red blood cell-derived extracellular vesicles with miR-204 mimic loading for pediatric neuroblastoma treatment

Red blood cell-derived extracellular vesicles with miR-204 mimic loading for pediatric neuroblastoma treatment

The Function of Different Subunits of the Molecular Chaperone CCT in the Microsporidium Nosema bombycis: NbCCTζ Interacts with NbCCTα

Identification and subcellular localization analysis of membrane protein Ycf 1 in the microsporidian Nosema bombycis

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