CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung.

Shipp, Margaret A.; Tarr, George E.; Chen, Changyan; Switzer, Stephanie N.; Hersh, Louis B.; Stein, Harald; Sunday, Mary E.; Reinherz, Ellis L.

doi:10.1073/pnas.88.23.10662

Cited by 189 publications

(177 citation statements)

References 45 publications

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“…However serum levels of ACE activity and the ACE levels in BCF showed an inverse correlation, which suggests that ACE in BCF is not derived by uptake from the circulation and probably originates from a local source. (Shipp et al, 1991) and as GRP and bombesin are potent growth-promoting factors for a wide variety of cancers (reviewed in Schrey and Patel, 1994) it seems possible that a reduction of enkephalinase expression by cancer cells is necessary for GRP to have a mitogenic effect. Indeed the growth of bombesin-dependent small-cell lung carcinomas is stimulated by enkephalinase inhibitors and reduced by exogenous enkephalinase (Shipp et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…(Shipp et al, 1991) and as GRP and bombesin are potent growth-promoting factors for a wide variety of cancers (reviewed in Schrey and Patel, 1994) it seems possible that a reduction of enkephalinase expression by cancer cells is necessary for GRP to have a mitogenic effect. Indeed the growth of bombesin-dependent small-cell lung carcinomas is stimulated by enkephalinase inhibitors and reduced by exogenous enkephalinase (Shipp et al, 1991). GRP is present in BCF (Weber et al, 1989) and is present in higher levels in type 1 fluids, leading to the suggestion that it is a factor in the increased risk associated with type 1 BCF (Lai et al 1990a).…”

Section: Discussionmentioning

confidence: 99%

“…ACE is capable of cleaving many peptides (Hooper. 1991) (Shipp et al, 1991;Ganju et al, 1994). and determining the effect of these inhibitors on tumour growth in in vivo models.…”

Section: Discussionmentioning

confidence: 99%

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Angiotensin-converting enzyme and enkephalinase in human breast cyst fluid

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Patton²,

Reed³

et al. 1996

Br J Cancer

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Angiotensin-converting enzyme and enkephalinase in human breast cyst fluid

Frame

Patton²,

Reed³

et al. 1996

Br J Cancer

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“…170 The inactivation of such peptides causes a reduction in the local concentrations available for receptor binding and signal transduction. 171 Therefore, loss of NEP results in the failure to inactivate the active peptides. 172 Recently, Papandreou et al 172 have shown that NEP expression and catalytic activity are lost in vitro in androgen-independent but not androgen-dependent prostate cancer cell lines.…”

Section: Neuropeptidesmentioning

confidence: 99%

Molecular and cellular biology of prostate cancer—the role of apoptosis as a target for therapy

Costa-Pereira

Cotter

1999

Prostate Cancer Prostatic Dis

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Despite the high incidence and mortality rate of prostate cancer, the molecular mechanisms underlying the progression of the disease remain to be fully elucidated. This paper reviews the current state of knowledge on prostatic carcinogenesis, focusing not only on the molecular genetics of prostate cancer, but also on the role of oncogenes, tumour suppressor genes, and growth factors. The remarkable ability of prostate cancer cells to survive androgen withdrawal and apoptosis are discussed, with particular emphasis on how apoptosis may be manipulated from a therapeutic viewpoint.

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“…The physiological role of the enzyme depends on available substrates in specific organs and cell types (Shipp and Look, 1993). The enzyme also regulates peptidemediated cellular proliferation by inactivating bombesin or other neuropeptides that are essential autocrine growth factors for certain human solid tumors (Burns et al, 1999;Cohen et al, 1996;Papandreou et al, 1998;Shipp et al, 1991).…”

mentioning

confidence: 99%

The Expression and Localization of Neutral Endopeptidase 24.11/CD10 in Human Gestational Trophoblastic Diseases

Ino

Suzuki

Uehara

et al. 2000

Laboratory Investigation

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SUMMARY:Neutral endopeptidase 24.11 (NEP)/CD10 is a cell-surface peptidase that hydrolyzes various bioactive peptides.NEP is distributed in both normal and neoplastic cells and plays a functional role by modulating cellular responses to peptide substrates. Recently, NEP has been shown to be expressed in normal placental trophoblasts, suggesting its physiological role during pregnancy. In the present study, we investigated the expression of NEP in hyperplastic and anaplastic trophoblasts in gestational trophoblastic diseases (GTDs). Flow cytometric analysis demonstrated that NEP was expressed in all choriocarcinoma cell lines examined. The NEP enzyme activity in these cell lines correlated with cell-surface protein levels and was abolished by the NEP inhibitor phosphoramidon. On immunoblot analysis, NEP protein was detected in both hydatidiform mole and choriocarcinoma tissues as a double band of 95 and 100 kDa similar to that of the normal placental tissues. Immunohistochemical analysis revealed that NEP was present on syncytiotrophoblasts, while no or very faint NEP immunoreactivity was observed on cytotrophoblasts in the normal placenta. Similarly, NEP in hydatidiform mole and invasive mole was localized on the membrane of syncytiotrophoblasts, but not on hyperplastic cytotrophoblasts. In contrast, in choriocarcinoma, NEP was highly expressed not only on syncytiotrophoblastic cells but also on invading anaplastic cytotrophoblasts. In addition, NEP was also expressed on intermediate trophoblasts in placental site trophoblastic tumors. In summary, this is the first study demonstrating the expression of NEP/CD10 in GTDs. The differential localization of NEP among various trophoblastic tumors suggests that NEP may play a functional role in the regulation of trophoblast transformation and human chorionic gonadotropin secretion. (Lab Invest 2000, 80: 1729 -1738.

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CD10/neutral endopeptidase 24.11 hydrolyzes bombesin-like peptides and regulates the growth of small cell carcinomas of the lung.

Cited by 189 publications

References 45 publications

Angiotensin-converting enzyme and enkephalinase in human breast cyst fluid

Angiotensin-converting enzyme and enkephalinase in human breast cyst fluid

Molecular and cellular biology of prostate cancer—the role of apoptosis as a target for therapy

The Expression and Localization of Neutral Endopeptidase 24.11/CD10 in Human Gestational Trophoblastic Diseases

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