Cd117 and cd34 staining patterns in childhood benign mammary lesions

Abstract

Objective: CD117 and CD34 are markers that have both been implied in cancer progression in adult breast lesions. This study was conducted in order to create a retrospective documentation and to analyze the expression patterns of these markers on childhood benign lesions along with a comparison with adult breast lesions' staining patterns. Material and Method:Nine fibroadenomas, 2 tubular adenomas, 1 mammary hamartoma, 2 gynecomastias, 1 benign phyllodes tumor were retrieved from pathology archives of two reference centers between 2005-2010.Results: CD117 staining was identified in the epithelium of all cases in fibroadenoma/tubular adenoma group and focally positive in 1 mammary hamartoma, 2 gynecomastias, and 1 benign phyllodes tumor. CD117 staining was detected in the stroma of 8 cases. Three fibroadenomas, 1 mammary hamartoma, 2 gynecomastias and 1 benign phyllodes tumor lacked stromal labelling for this marker. All cases were strongly and diffusely positive for CD34 except the benign phyllodes tumor case. This case presented marked loss of stromal CD34 staining when compared to the surrounding stroma. Additionally, pseudoangiomatous stromal hyperplasia was noted in 2 gynecomastias and in the peritumoral stroma of benign phyllodes tumor case. Conclusion:Our study demonstrated that fibroadenoma was the most commonly encountered breast lesion in childhood and that adolescent fibroadenomas showed similar staining patterns for CD117 and CD34 as for adult counterparts. On the other hand, different expression patterns of CD117 and CD34 between adenoma group and the gynecomastias and benign phyllodes tumor group may implicate different mechanisms of development and tumorigenesis among these groups.Key Words: Adolescent, Child, Breast, CD117 antigen, CD34 antigen ÖZ Amaç: CD117 ve CD34'ün yetişkin meme lezyonlarında kanser progresyonunda rolü olduğu bilinmektedir. Bu çalışma nadir görülen pediatrik meme lezyonlarına ait geriye dönük bir dokümantasyon oluşturma ve bu belirteçlerin çocukluk çağı benign lezyonlarındaki ekspresyonlarını gözlemlemek ve erişkin lezyonlarında bilinen paternlerle karşılaştırma yapabilmek üzere kurgulanmıştır. Gereç ve Yöntem:İki merkeze ait 2005-2010 dönemi patoloji arşivi taranarak 9 fibroadenoma, 2 tübüler adenoma, 1 meme hamartomu, 2 jinekomasti, 1 benign fillodes tümör olgusu çalışmaya alınmıştır. Bulgular: CD117 fibroadenoma/tübüler adenoma grubundaki tüm olgularda epitelyal pozitivite göstermiş ve 1 meme hamartomu, 2 jinekomasti ve 1 benign fillodes tümör olgusunda fokal olarak pozitif bulunmuştur. Toplam 8 olguda stromal CD117 ekspresyonu gözlenmiştir. Benign fillodes tümör olgusu dışında bütün olgular CD34 ile kuvvetli ve yaygın stromal pozitivite göstermiştir. Bu olguda tümör dışı alanlarda kuvvetli pozitivite mevcutken, tümörde CD34 ekspresyonu gözlenmemiştir. Ayrıca iki jinekomasti olgusunda ve benign fillodes tümör olgusunun peritumöral stromasında pseudoanjiyomatöz stromal hiperplazi izlenmiştir.Sonuç: Çalışmamızda, çocukluk çağının en sık gözlenen meme lezyonunun fibroa...

Malignant phyllodes tumors display mesenchymal stem cell features and aldehyde dehydrogenase/disialoganglioside identify their tumor stem cells

“…Paraffin blocks from 47 individual patients, four fresh specimens of malignant PT and their xenografted tumors, were examined by immunohistochemical staining for the following markers: CD44 (HCAM), CD29 (integrin β-1), CD106 (VCAM-1), CD166 (ALCAM), CD105 (Endoglin), CD90 (Thy-1) [17-20], GD2 (ganglioside) [11], CD117(c-kit receptor) [21], ALDH1 [15], embryonic stem cell marker Oct-4 (Octamer-4 in abbreviation), CD34 [22], CD10, p53, p63, Ki-67, Bcl-2 [23] and vimentin [24]. Mesenchymal progenitor cell-line HS-5 was chosen as a control.…”

Utility of Ki-67, CD10, CD34, p53, CD117, and Mast Cell Content in the Differential Diagnosis of Cellular Fibroadenomas and in the Classification of Phyllodes Tumors of the Breast

“…A cutoff point was not used here, but rather the association between the immunoreactivity score and tumor types. In a study on breast lesions in adolescents, Kaçar et al 32 reported that, in all 9 evaluated fibroadenomas (6 of which were cellular type), there was diffuse epithelial expression of CD117, being only focal in the epithelium of the benign PTB of the series. Yared et al 33 presented a possible explanation for this finding, where a strong and diffuse expression of CD117 in the epithelium of normal breast tissue was found, which progressively diminished in hyperplastic lesions, fibroadenomas, and papillomas until carcinomas, suggesting a loss of epithelial expression as the neoplastic progression occurs.…”

Differential mast cell density in spectrum of benign neoplasms of breast: potential for patient stratification and personalised treatment approach

“…A subset of mast cells expresses SCF (Stem cell Factor) which can bind to its receptor C-KIT (also known as CD 117; a type of receptor tyrosine kinase). It has been shown that increased C-KIT expression noted in these spectrum of benign breast neoplasms in varying proportion is a mast cell phenomenon and mast cell derived mediators may play a role in progression to borderline and malignant phyllodes tumours [16][17][18][19].…”