CD117/c-KIT Expression in Phyllodes Tumor of the Breast and Its Correlation With Morphology and Clinical Outcome

Abstract

Introduction Breast phyllodes tumor’s (PT) clinical behavior is difficult to predict on histology. There is limited amount of data available on the role of biological markers. The objective of this study was to assess the expression of CD117 (c-KIT) in PT of the breast and its relationship with morphology and clinical outcome. Methods A total of 78 patients having available clinical records between 2004 and 2014 with breast PT were retrieved from the cancer registry at Shaukat Khanum Memorial Cancer Hospital and Research Centre, Lahore and were followed up. Immunohistochemical studies were performed on all the cases using mono­clonal antibody CD117 (DAKO A4502) using Leica Bond-Max automated biosystem (Leica Biosystems, Melbourne, Australia). Staining proportion and intensity of both epithelial and stromal elements were analyzed. Evaluation of the protein expression was determined and scored. Results Patients’ mean age was 45.13 ± 11.4 years. Thirty-nine (50.0%) patients had tumor on right side, 38 (48.7%) had on left and only one (1.3%) was bilateral. Thirty-two (41.0%) patients had benign PT, 21 (26.9%) had borderline and 25 (32.1%) had malignant PT. Negative CD117 expression was observed in 20 (62.5%), eight (38.0%) and 12 (48.0%) of benign, borderline and malignant PTs, respectively. Positive CD117 expression was observed in 12 (37.5%) benign, 13 (61.9%) borderline and 13 (52.0%) malignant PTs. There was insignificant association between CD117 expression with respect to tumor sub-type, patient’s age and clinical behavior (p-value > 0.05). Conclusion CD117 expression was not found to be associated with tumor type and was not associated with increased risk of recurrence in this sample of patients. Further work is needed to better understand the prognostic value of CD117 expression in breast PTs.

Significance of Estrogen/Progesterone Receptor Expression in Metaplastic Breast Carcinoma

“…The formalin-fixed-paraffin embedded blocks were then sliced into 3-4 μm sections. Sliced sections were transferred onto L-lysin-treated slides, sequentially treated with xylene, alcohol, and water, and stained with hematoxylin and eosin [10]. These slides were examined by a senior histopathologist at the institute.…”

The Genetic Landscape of Fibroepithelial Lesions of the Breast

“…It has been shown in some studies that CD117 expression increases from benign to malignant PTs and that CD117 stromal-positive PTs tended to have a shorter recurrence-free survival. [30][31][32][33] Other studies, however, have shown no statistically significant differences between CD117 immunoreactivity and PT grades; [34][35][36] however, the number of investigations applying CD117 IHC to PTs in this context are few, the value of incorporating CD117 IHC in into the practice of grading PTs remains unclear.…”