CD133+ cells from human peripheral blood promote corticospinal axon regeneration

Yamamoto, Risako; Ishikawa, Masakazu; Tanaka, Nobuhiro; Kamei, Naosuke; Nakanishi, Kazuyoshi; Sasaki, Hirofumi; Nakamae, Toshio; Mochizuki, Yu; Asahara, Takayuki; Ochi, Mitsuo

doi:10.1097/wnr.0b013e3282ff6396

Cited by 10 publications

(28 citation statements)

References 23 publications

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“…13,14 The CD133 + cells were labeled with a magnetic bead, and isolated from human peripheral blood using a magnetic cell separation instrument. 15 We hypothesized that immunomagnetic CD133…”

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confidence: 99%

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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Skeletal muscle injuries often leave lasting functional damage or pain. Muscle injuries are routinely treated conservatively, but the most effective treatment to promote the repair of injured muscles has not yet been established. Our previous report demonstrated that human peripheral blood-derived CD133+ cell transplantation to rat skeletal muscle injury models inhibited fibrosis and enhanced myogenesis after injury. However, the acquisition of a sufficient number of cells remains the limitation for clinical application, as the CD133 + population is rare in human blood. In this study, we applied a magnetic cell targeting system to accumulate transplanted cells in the muscle injury site and to enhance the regenerative effects of CD133 + cell transplantation, focusing on the fact that CD133 + cells are labeled with a magnetic bead for isolation. For the magnetic cell targeting, the magnet field generator was set up to adjust the peak of the magnetic gradient to the injury site of the tibialis anterior muscle, and 1 · 10 4 human peripheral blood CD133 + cells were locally injected into the injury site. This cell number is 10% of that used in the previous study. In another group, the same number of CD133 + cells was injected without magnetic force. The CD133 + cells transplanted with the magnetic force were more accumulated in the muscle injury site compared with the CD133 + cells transplanted without the magnetic force. In addition, the transplantation of CD133+ cells under the magnetic control inhibited fibrous scar formation and promoted angiogenesis and myogenesis, and also upregulated the mRNA expression of myogenic transcription factors, including Pax7, MyoD1 and Myogenin. However, the transplantation of CD133 + cells without the magnetic force failed to demonstrate these effects. Thus, our magnetic cell targeting system enables transplantation of a limited number of CD133 + cells to promote the repair of skeletal muscle injury.

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confidence: 99%

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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“…Life expectancies range from 15 to 47 months after presentation [8,6] even after Riluzole treatment. Stem cell transplantation is a potential therapeutic strategy for ALS patients, as well as for other neurodegenerative disorders [41,43,44,45,[34][35][36][37][38]22] that CD133+ stem cells isolated from the peripheral blood can differentiate into neurons [27,28,31].…”

Section: Discussionmentioning

confidence: 99%

“…These cells can be easily isolated from the peripheral blood or bone marrow (BM) [25][26][27][28][29][30]. It has been demonstrated that stem cells isolated from peripheral blood of ALS patients, can be induced to differentiate into preneurons or stem cells isolated from human BM are capable to differentiate into neurons [31].…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that stem cells isolated from peripheral blood of ALS patients, can be induced to differentiate into preneurons or stem cells isolated from human BM are capable to differentiate into neurons [31]. These differentiated cells express nestin, neuron-specific enolase, neuron-specific nuclear protein, glial fibrillary acidic protein, neurofilaments, TAU, NURR1, and neuron-specific tubulin 1 [27][28][29][31][32][33]. Some studies have reported that these cells have synaptic transmission capacities [32,33].…”

Section: Introductionmentioning

confidence: 99%

“…No animal model reproduces all the salient features of ALS particularly the involvement of the pyramidal tract. Furthermore, mesenchymal stem cell differentiate into neurons in in vitro and in vivo studies [27][28][29]. Therefore some researchers have attempted stem-cell-based approaches for the treatment of ALS patients.…”

Section: Introductionmentioning

confidence: 99%

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Increase of Pyramidal Tract Fractional Anisotropy on MRI after Stem Cell Transplantation in ALS Patients

HR¹,

Garza²

2014

J Neurol Neurophysiol

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Background and Purpose: Amyotrophic lateral sclerosis (ALS) is characterized by the selective death of motor neurons. Cortical neuron loss is associated with axonal degeneration along specific white matter tracts. Fractional anisotropy (FA) determined by Magnetic Resonance (MR) might detect integrity or changes in brain white matter pathways. This study was aimed to analyze pyramidal tract changes in ALS patients submitted to stem cell transplantation into the frontal motor cortices.

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Acceleration of Skeletal Muscle Regeneration in a Rat Skeletal Muscle Injury Model by Local Injection of Human Peripheral Blood-Derived CD133-Positive Cells

et al. 2009

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Muscle injuries in sport activities can pose challenging problems in traumatology and sports medicine. The best treatment for muscle injury has not been clearly established except for the conservative treatment that is routinely performed. We investigated the potential of human adult CD1331 cells to contribute to skeletal muscle regeneration in an athymic rat model. We tested whether CD1331 cells locally transplanted to the skeletal muscle lacerated models could (a) induce vasculogenesis/angiogenesis, (b) differentiate into endothelial and myogenic lineages, and (c) finally promote histological and functional skeletal myogenesis. Granulocyte colony stimulating factor-mobilized peripheral blood (PB) CD1331 cells, PB mononuclear cells, or phosphate-buffered saline was locally injected after creating a muscle laceration in the tibialis anterior muscle in athymic rats. After treatment, histological and functional skeletal myogenesis was observed significantly in the CD1331 group. The injected CD1331 cells differentiated into endothelial and myogenic lineages. Using real-time polymerase chain reaction analysis, we found that the gene expressions related to microenvironment conduction for host angiogenesis, fibrosis, and myogenesis were ideally up/downregulated. Our results show that CD1331 cells have the potential to enhance the histological and functional recovery from skeletal muscle injury rather via indirect contribution to environment conduction for muscular regeneration. It would be relatively easy to purify this cell fraction from PB, which could be a feasible and attractive autologous candidate for skeletal muscle injuries in a clinical setting. These advantages could accelerate the progression of cell-based therapies for skeletal muscle injuries from laboratory to clinical implementation. STEM CELLS 2009;27:949-960 Disclosure of potential conflicts of interest is found at the end of this article.

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CD133+ cells from human peripheral blood promote corticospinal axon regeneration

Cited by 10 publications

References 23 publications

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Increase of Pyramidal Tract Fractional Anisotropy on MRI after Stem Cell Transplantation in ALS Patients

Acceleration of Skeletal Muscle Regeneration in a Rat Skeletal Muscle Injury Model by Local Injection of Human Peripheral Blood-Derived CD133-Positive Cells

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CD133+ cells from human peripheral blood promote corticospinal axon regeneration

Cited by 10 publications

References 23 publications

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Increase of Pyramidal Tract Fractional Anisotropy on MRI after Stem Cell Transplantation in ALS Patients

Acceleration of Skeletal Muscle Regeneration in a Rat Skeletal Muscle Injury Model by Local Injection of Human Peripheral Blood-Derived CD133-Positive Cells

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Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration