CD138-negative clonogenic cells are plasma cells but not B cells in some multiple myeloma patients

CD19−CD45low/−CD38high/CD138+ plasma cells enrich for human tumorigenic myeloma cells

“…Furthermore, our studies show that CD19 þ CD38 low/ À B cells from myeloma patients are able to engraft in human bone grafts, resulting in the repopulation of polyclonal B cells. As memory B cells have the ability to self-renew, 44 we speculate that bone marrow-residing polyclonal CD19 47 reported that the CD138 À myeloma cells in human myeloma cell lines and in some multiple myeloma patients maintain colony-forming ability. In particular, Hosen et al…”

Differential Protein Expression Profile Between CD20 Positive and Negative Cells of the NCI-H929 Cell Line

“…Moreover, CD138-cells were only found in few human myeloma cell lines and were not relevant to the clonogenicity (Jakubikova et al, 2011). It was found that CD138-clonogenic cells are plasma cells rather than B cells, and that MM plasma cells including CD138(-) and CD138(+) cells have the potential to propagate MM clones in vivo in the absence of CD19(+) B cells (Hosen et al, 2012). In patients, it was believed that CD20-CD138+ was the origin of clonogenic myeloma cells (Chiron et al, 2012).…”

“…However, the phenotype of clonogenic myeloma cells remains a matter for debate. It was showed that CD20+ and CD138-cells were not the mark of clonogenic cells (Jakubikova et al, 2011;Chiron et al, 2012;Hosen et al, 2012). Moreover, some study found that NCI-H929 cells did not contain CD20 positive cells and suggested that CD20 positive cells were not associated with a cancer stem cell phenotype (Paíno et al, 2012).…”

Multiple myeloma-initiating cells

“…B cells were FACS-sorted from PB or BM cells from MM patients and transplanted directly into BM of NOD/Shi-scid,IL-2Rcnull (NOG) mice or intravenously to new born pups of NOG mice [16]. Engraftment of human MM cells was monitored by measuring human immunoglobulin light chain (IgL) j and k in serum of the recipient mice, but no human IgL was detected at any time.…”