CD147 Levels in Blood and Adipose Tissues Correlate with Vascular Dysfunction in Obese Diabetic Adults

Abstract

CD147 is a glycoprotein that stimulates the production of matrix metalloproteinases (MMPs), known contributors to cardiovascular risk. The activity of CD147 protein depends on its glycosylation. However, it is unclear whether CD147 protein expression or glycosylation are influenced by the diabetic milieu characterized by hyperglycemia and abundant glycation-end-products (AGEs). We examined the circulating and visceral adipose tissue (VAT) levels of CD147 and their correlation with vascular function in obese, obese diabetic, and non-obese controls (n = 40, each). The circulating levels of CD147 and the glycosylated CD147 protein in VAT were considerably higher in obese, particularly obese diabetic subjects compared to controls. Obese diabetics had the lowest brachial and arteriolar vasoreactivity and the highest carotid pulse-wave velocity (PWV, a measure of arterial stiffness) among the three groups. CD147 correlated positively with body mass index (BMI), total and visceral fat mass, PWV, and plasma levels of glucose, insulin, MMPs, and AGEs and negatively with brachial artery and VAT-arteriolar vasoreactivity and nitric oxide production. Multivariate regression revealed that BMI, body fat mass, insulin, and glucose levels significantly predicted CD147. Our data suggest that higher levels of CD147 in obese subjects, particularly those with diabetes, are linked to vascular dysfunction and several cardiometabolic risk factors.

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“…Following this, infrared IRDye-labeled secondary antibodies (LI-COR Biosciences) were applied for 1 h at room temperature and protected from light. The membranes were washed with TBS + 0.1% Tween-20, dried at room temperature, and imaged in the appropriate channel (700 nm for IRDye680TM antibodies, 800 nm for IRDye800TM antibodies) using an Odyssey Clx infrared imaging system [33]. The quantification of images was performed using Image Studio ver.…”

The potential link between Covid‐19 and multiple myeloma: A new saga

“… 121 A high level of CD147 in the plasma of obese patients is correlated with vascular dysfunction and linked with cardiometabolic risk factors. 121 This might explain high mortality and Covid‐19 severity in old age with comorbidities. A cohort study involving 119 Covid‐19 patients compared to 69 controls illustrated that platelet expression of CD147 was increased, causing inflammation and thrombosis through induction release of P selectin and HMGB1, which predict worse outcomes and Covid‐19 severity.…”

“…As well, CD147 expression is augmented in various cardiometabolic disorders, including obesity, hypertension, diabetes, thrombosis, and chronic kidney diseases 121 . A high level of CD147 in the plasma of obese patients is correlated with vascular dysfunction and linked with cardiometabolic risk factors 121 . This might explain high mortality and Covid‐19 severity in old age with comorbidities.…”

“…Expression of CD147 in the endothelial cell is increased with advanced age. As well, CD147 expression is augmented in various cardiometabolic disorders, including obesity, hypertension, diabetes, thrombosis, and chronic kidney diseases 121 . A high level of CD147 in the plasma of obese patients is correlated with vascular dysfunction and linked with cardiometabolic risk factors 121 .…”