CD147 promotes melanoma cell growth via SOX4-mediated glycolytic metabolism

Sun, Xinwei; Yang, Pengfei; Jiang, Yuan

doi:10.4314/tjpr.v19i12.6

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Melanoma is a highly aggressive cancer that is caused by exposure to ultraviolet radiation [1,2]. Melanomas tend to metastasize into other organs, including the liver, lung, brain, and bone [3].…”

Section: Introductionmentioning

confidence: 99%

SPAG9 promotes the migration and invasion of melanoma cells by regulating the MAPK pathway

Liu¹,

Zhu²,

You³

et al. 2022

Trop. J. Pharm Res

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Purpose: To determine the regulatory role of sperm-associated antigen 9 (SPAG9) in melanoma cell growth. Methods: Immunohistochemical analysis was performed to investigate SPAG9 expression in melanoma tissues. Western blot was used to evaluate SPAG9 expression in melanoma tissues and cells. Melanoma cells were transfected with an siRNA targeting SPAG9 or a SPAG9 overexpression vector. Cell migration and invasion were examined by wound healing and transwell assays. The effects of SPAG9 on the epithelial-mesenchymal transition and mitogen-activated protein kinase (MAPK) pathway in melanoma cells were assessed by western blot. Results: SPAG9 expression was enhanced in melanoma tissues and cells. SiRNA-mediated silencing of SPAG9 repressed melanoma cell migration and invasion, and SPAG9 overexpression contributed to melanoma cell metastasis. In melanoma cells transfected with siRNA-SPAG9, E-cadherin expression decreased while vimentin and matrix metalloproteinase (MMP)-2/9 expression increased. However, ectopic expression of SPAG9 reversed this expression of E-cadherin, vimentin, and MMP-2/9. Silencing of SPAG9 decreased the phosphorylation of MAPKs, including p-p38, p-ERK and p-JNK, in melanoma cells. SPAG9 overexpression upregulated phosphorylation of MAPKs. Conclusion: SPAG9 promotes the migration and invasion of melanoma cells by activating the MAPK pathway.

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Section: Introductionmentioning

confidence: 99%

SPAG9 promotes the migration and invasion of melanoma cells by regulating the MAPK pathway

Liu¹,

Zhu²,

You³

et al. 2022

Trop. J. Pharm Res

View full text Add to dashboard Cite

show abstract

“…Melanoma is a deadly form of skin cancer that results from the malignant transformation of melanocytes in the epidermis [1]. Although melanoma has the lowest incidence among skin cancers, it is the most malignant and aggressive skin cancer and accounts for 75 % of skin cancer-related deaths [2].…”

Section: Introductionmentioning

confidence: 99%

Centrosomal protein55 enhances melanoma cell proliferation, invasion and migration through PI3K/AKT signaling

Hua¹,

Heng²

2022

Trop. J. Pharm Res

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Purpose: To investigate the role of centrosomal protein55 (CEP55) in melanoma. Methods: The CEP55 expression in human melanoma cells and normal human melanocytes were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot. Cell proliferation was evaluated by colony formation and CCK8 assays, while cell cycle was assessed by flow cytometry. Transwell assay was used to investigate cell migration and invasion. Results: The CEP55 protein expression was higher in A-2058 and A375 melanoma cells than in normal HEMn-LP cells. Overexpression of CEP55 increased melanoma cell viability and the number of melanoma cell colonies (p < 0.01), while CEP55 silencing reduced melanoma cell viability and proliferation. Overexpression of CEP55 promoted cell cycle (p < 0.01) via decreased p21 expression, but increased CyclinD1 and CDK1 expression. Knockdown of CEP55 enhanced p21 expression and reduced CyclinD1 and CDK1 expression to induce cell cycle arrest. Knockdown of CEP55 suppressed melanoma cell migration and invasion by downregulating p-AKT and p-PI3K. Conclusion: Centrosomal protein55 functions as an oncogene in melanoma by activating PI3K/AKT signaling. Therefore, CEP55 might be a promising target for melanoma diagnosis and treatment.

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Lung Cancer Gene Regulatory Network of Transcription Factors Related to the Hallmarks of Cancer

Otálora‐Otálora

López‐Kleine

Rojas

2023

CIMB

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The transcriptomic analysis of microarray and RNA-Seq datasets followed our own bioinformatic pipeline to identify a transcriptional regulatory network of lung cancer. Twenty-six transcription factors are dysregulated and co-expressed in most of the lung cancer and pulmonary arterial hypertension datasets, which makes them the most frequently dysregulated transcription factors. Co-expression, gene regulatory, coregulatory, and transcriptional regulatory networks, along with fibration symmetries, were constructed to identify common connection patterns, alignments, main regulators, and target genes in order to analyze transcription factor complex formation, as well as its synchronized co-expression patterns in every type of lung cancer. The regulatory function of the most frequently dysregulated transcription factors over lung cancer deregulated genes was validated with ChEA3 enrichment analysis. A Kaplan–Meier plotter analysis linked the dysregulation of the top transcription factors with lung cancer patients' survival. Our results indicate that lung cancer has unique and common deregulated genes and transcription factors with pulmonary arterial hypertension, co-expressed and regulated in a coordinated and cooperative manner by the transcriptional regulatory network that might be associated with critical biological processes and signaling pathways related to the acquisition of the hallmarks of cancer, making them potentially relevant tumor biomarkers for lung cancer early diagnosis and targets for the development of personalized therapies against lung cancer.

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CD147 promotes melanoma cell growth via SOX4-mediated glycolytic metabolism

Cited by 3 publications

References 20 publications

SPAG9 promotes the migration and invasion of melanoma cells by regulating the MAPK pathway

SPAG9 promotes the migration and invasion of melanoma cells by regulating the MAPK pathway

Centrosomal protein55 enhances melanoma cell proliferation, invasion and migration through PI3K/AKT signaling

Lung Cancer Gene Regulatory Network of Transcription Factors Related to the Hallmarks of Cancer

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