CD147 regulates vascular endothelial growth factor—A expression, tumorigenicity, and chemosensitivity to curcumin in hepatocellular carcinoma

Li, Jia; Wang, Huaxin; Qu, Shen; Xing, Miao; Zhang, Jianing

doi:10.1002/iub.11

Cited by 31 publications

(21 citation statements)

References 26 publications

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“…MMPs may also participate in late events during cancer metastatic spread, when cancer cells enter, survive and exit blood vessels or lymphatics. CD147 also can affect expressions of vascular endothelial growth factor (VEGF) at RNA and protein level, as well as promote the growth of the tumor vascular system [30,31]. Moreover, CD147 is a multifunctional protein whose functional diversity may relate to distinct post-translational modifications.…”

Section: Discussionmentioning

confidence: 98%

CD147 Expression Indicates Unfavourable Prognosis in Prostate Cancer

Han

Qin

et al. 2008

Pathol. Oncol. Res.

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Extracellular matrix metalloproteinase inducer (EMMPRIN, also named as CD147) is a multifunctional membrane glycoprotein over-expressed in many kinds of human solid tumors. It has been demonstrated to be involved in tumor invasion and angiogenesis. The aim of this study was to analyze the clinicopathological characteristics of the expression of CD147 in human prostate cancer (PCa), and to evaluate its clinical significance in the histologic classification and prognosis of PCa. CD147 protein expression in paraffin-embedded specimens gathered from 62 cases of PCa and 30 cases of benign prostatic hyperplasia (BPH) were detected by the method of immunohistochemistry. The association of CD147 protein expression with the clinicopathological characteristics and with the prognosis of PCa was subsequently assessed. CD147 expression were positively expressed in 51/62 (82.3%) of PCa and 4/30 (13.3%) of BPH cases, respectively. The positive expression rate of CD147 in PCa tissues was significantly higher than that in BPH. The positive expression of CD147 was dramatically associated with TNM grade (p < 0.001), the depth of the prostatic wall invasion (p = 0.008), GLEASON Score (p = 0.001) and Histologic grade (p = 0.001). The patients with CD147 expression were associated with a poor prognosis of PCa (p = 0.01) and the survival rate of the patients with a strong positive expression of CD147 was the lowest (p = 0.01). The results suggest that the expression of CD147 may be an important feature of PCa and the detection of its expression may benefit us in the prediction of the prognosis of PCa.

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Section: Discussionmentioning

confidence: 98%

CD147 Expression Indicates Unfavourable Prognosis in Prostate Cancer

Han

Qin

et al. 2008

Pathol. Oncol. Res.

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“…In colorectal cancer patients, MDR was also an important cause of treatment failure and mortality. In many different ways, the protein of CD147 could regulate the chemosensitivity of certain chemotherapeutic drugs (33,34). The anticancer drugs cisplatin, paclitaxel, oxaliplatin and gemcitabine are widely used, and potent in head and neck cancer, and also often used in treatment of colorectal ).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo

Pan

et al. 2013

International Journal of Oncology

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Abstract.We investigated the effect of CD147 silencing on HT29 cell proliferation and invasion. We constructed a novel short hairpin RNA (shRNA) expression vector pYr-mir30-shRNA. The plasmid was transferred to HT29 cells. The expression of CD147, MCT1 (lactate transporters monocarboxylate transporter 1) and MCT4 (lactate transporters monocarboxylate transporter 4) were monitored by quantitative PCR and western blotting, respectively. The MMP-2 (matrix metalloproteinase-2) and MMP-9 (matrix metalloproteinase-9) activities were determined by gelatin zymography assay, while the intracellular lactate concentration was determined by the lactic acid assay kit. WST-8 assay was used to determine the HT29 cell proliferation and the chemosensitivity. Invasion assay was used to determine the invasion of HT29 cells. In addition, we established a colorectal cancer model, and detected CD147 expression in vivo. The results showed that the expression of CD147 and MCT1 was significantly reduced at both mRNA and protein levels, and also the activity of MMP-2 and MMP-9 was reduced. The proliferation and invasion were decreased, but chemosensitivity to cisplatin was increased. In vivo, the CD147 expression was also significantly decreased, and reduced the tumor growth after CD147 gene silencing. The results demonstrated that silencing of CD147 expression inhibited the proliferation and invasion, suggesting CD147 silencing might be an adjuvant gene therapy strategy to chemotherapy.

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“…In cancer cells, CD147 regulates chemosensitivity to some chemotherapeutic drugs such as paclitaxel and curcumin (16,36). Regulation of CD147 expression is considered to be a novel approach to overcome resistance to multiple anticancer drugs.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of CD147 gene expression via RNA interference reduces tumor cell invasion, tumorigenicity and increases chemosensitivity to cisplatin in laryngeal carcinoma Hep2 cells

Zhu

Pan²,

He³

et al. 2011

Oncol Rep

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Abstract. CD147, also named extracelluar matrix metalloproteinase inducer (EMMPRIN), is a member of the immunoglobulin family and a glycoprotein enriched on the surface of tumor cells, which promotes invasion, metastasis, growth and survival of malignant cells, and is known to confer resistance to some chemotherapeutic drugs. To determine the possible role of CD147 in the invasive properties of laryngeal carcinoma, we used an RNA interference approach to silence CD147 expression in the Hep2 cell line at high levels of CD147 expression. Our results showed that CD147 expression was significantly impeded at both mRNA and protein levels, which resulted in a decrease of the Hep2 invasion activity in vitro and tumorigenicity in nude mice. The suppression of CD147 expression also sensitized cells to cisplatin. Our current results indicated that CD147 was a laryngeal carcinoma-related gene and CD147 might be a potential target for therapeutic anti-cancer drugs. IntroductionLaryngeal carcinoma is the 11th commonest form of cancer in men worldwide, with 121,000 new cases in 1985 (1). It is one of the most common malignancies in Europe, with about 52,000 new cases per year (2), approximately 9,500-11,000 new cases of laryngeal cancer are estimated to occur yearly in the United States (3,4). It is also very common in the northeast of China in recent years, and has shown increasing tendency (5). Laryngeal carcinoma especially at late-stage is associated with high morbidity and poor long-term survival due to the absence of effective treatment methods. Thus, better understanding of molecular mechanisms underlying proliferation, invasion and survival of laryngeal carcinoma is critical for the development of optimal therapeutic modalities. Recent studies suggest that CD147 is highly expressed in laryngeal carcinomas (6,7). CD147 (also known as EMMPRIN, basigin, M6 and tumor cell derived collagenase stimulatory factor), a highly glycosylated transmembrane protein that belongs to the immunoglobulin superfamily (8), is widely expressed and enriched on the surface of many malignant tumor cells. These cancers include breast cancer, lymphoma, oral squamous cell carcinoma, glioma, melanoma, lung, and bladder and kidney carcinomas (9-11). CD147 plays an important role in tumor formation and invasion/metastasis in both animal models (12) and cancer patients (13).The metastatic potential of cancerous cells is dependent upon their ability to disengage themselves from environmental cues that serve to regulate and constrain them. CD147 is found overexpressed in a number of cancerous specimens, coupled with its ability to induce matrix metalloproteinase (MMPs) expression (14). MMPs are a family of zincdependent endopeptidases which currently consists of more than 25 members. According to sequence homologies and related substrate preferences, MMPs can be divided into 5 subfamilies, collagenases, gelatinases, tromelysins, membrane-type MMPs, and others. MMPs expression allows degradation of the basement membrane that are believed to affect tumo...

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CD147 regulates vascular endothelial growth factor—A expression, tumorigenicity, and chemosensitivity to curcumin in hepatocellular carcinoma

Cited by 31 publications

References 26 publications

CD147 Expression Indicates Unfavourable Prognosis in Prostate Cancer

CD147 Expression Indicates Unfavourable Prognosis in Prostate Cancer

Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo

Inhibition of CD147 gene expression via RNA interference reduces tumor cell invasion, tumorigenicity and increases chemosensitivity to cisplatin in laryngeal carcinoma Hep2 cells

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