CD155 Is Involved in Negative Selection and Is Required To Retain Terminally Maturing CD8 T Cells in Thymus

Qiu, Quan; Ravens, Inga; Seth, Sebastian; Rathinasamy, Anchana; Maier, Michael K.; Davalos-Misslitz, Ana; Förster, Reinhold; Bernhardt, Günter

doi:10.4049/jimmunol.0900062

Cited by 14 publications

(24 citation statements)

References 57 publications

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“…Additionally, CD155 is a ligand for the Ig-like receptors DNAM-1 (CD226), Tactile (CD96), and T cell immunoreceptor with Ig and ITIM domains (TIGIT) on T cells and NK cells (18)(19)(20)(21)(22). Mice deficient in CD155 show diminished humoral immune responses and thymic retention of mature CD8 + cells (16,(23)(24)(25). Although several studies have demonstrated various functions of CD155, particularly on nonhematopoietic cells, the role of CD155 in lymphocyte function remains undefined.…”

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confidence: 99%

CD155 (PVR/Necl5) Mediates a Costimulatory Signal in CD4+ T Cells and Regulates Allergic Inflammation

Yamashita-Kanemaru

Takahashi

Wang

et al. 2015

The Journal of Immunology

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Although Th1 and Th2 cells are known to be involved in allergic inflammatory diseases, the molecular mechanisms underlying their differentiation are incompletely understood. In this study, we identified CD155 as a costimulatory molecule on CD4+ T cells. Importantly, CD155-mediated signaling induced Th1 development in both humans and mice, as evidenced by production of IFN-γ and upregulation of Tbx21 transcription; these effects were independent of IL-12 but dependent on NF-κB–induced autocrine IFN-γ that triggered positive feedback via STAT1 activation. Mice genetically deficient in CD155 or treated with anti-CD155 Ab exhibited attenuated Th1-type contact hypersensitivity. Thus, CD155 plays an important regulatory role in helper T cell differentiation and allergic diseases.

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confidence: 99%

CD155 (PVR/Necl5) Mediates a Costimulatory Signal in CD4+ T Cells and Regulates Allergic Inflammation

Yamashita-Kanemaru

Takahashi

Wang

et al. 2015

The Journal of Immunology

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“…12 CD155 knockout mice are characterized by reduced accumulation of CD8 single-positive thymocytes and by defective humoral immune responses in response to oral vaccination. 13,14 With regard to ECs, antibody-mediated neutralization of CD155 prevents monocyte and T-cell receptor-dependent effector memory T-cell transendothelial migration in vitro. 15,16 Most of the immunologic effects of CD155 are mediated by its interaction with DNAX accessory molecule-1 (DNAM-1) (also called CD226) or CD96 on the surface of leukocytes.…”

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confidence: 99%

“…In addition to ECs, it is interesting to note that we did not observe CD155 expression on resting human CD8 T cells, although CD155 expression has been documented on resting CD8 T cells in mice. 13 Systemic elimination of CD155 in mice results in a reduction in the number of mature CD8 SP thymocytes and defects in antibody class-switching in response to oral administration of antigen 13,14,28 . Furthermore, DNAM-1 expression on mouse CD8 T cells is needed for optimal CD8 T-cell proliferation and development of cytotoxic effector function in response to activation by B cells that overexpress CD155.…”

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CD155 on Human Vascular Endothelial Cells Attenuates the Acquisition of Effector Functions in CD8 T Cells

Escalante

Rossum

Lee

et al. 2011

ATVB

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Objective-CD155 is a cell surface protein that has recently been described to exert immune regulatory functions. We have characterized the expression of CD155 on human vascular endothelial cells (ECs) and examined its role in the regulation of T-cell activation. Methods and Results-CD155 was expressed on resting human vascular ECs and was upregulated in an interferon-␥ (IFN␥)-dependent manner. When the function of CD155 in regulating T-cell activation was examined, antibodymediated neutralization of CD155 did not affect CD8 T-cell proliferation in response to stimulation with ECs. However, neutralization of CD155 activity or small interfering RNA-mediated inhibition of CD155 expression in ECs increased expression of IFN␥ and cytotoxic effector function in activated CD8 T cells. Key Words: cytokines Ⅲ endothelium Ⅲ immune system Ⅲ CD155 Ⅲ T cell H uman vascular endothelial cells (ECs) act as semiprofessional antigen-presenting cells and, in this way, regulate immune responses by presenting antigenic peptides and cell surface proteins to T cells in peripheral tissues. 1,2 This immunoregulatory property of the endothelium is established in the control T-cell responses in solid organ transplantation and may regulate immune responses in autoimmunity and some cardiovascular diseases. 1 With regard to the expression of cell surface proteins, human ECs provide positive costimulatory signals to T cells mainly through leukocyte functional antigen-3, intercellular adhesion molecule-1, CD154, 41BB ligand, inducible co-stimulator ligand, and OX40 ligand. 3 These signals are required for the activation of memory CD4 and CD8 T cells by ECs. In contrast, ECs also express cell surface proteins, such as PD-L1, that inhibit T cell responses. 4,5 The expression of immune regulatory proteins on ECs is controlled by cytokines. In particular, interferon-␥ (IFN␥) is especially pertinent to EC immunobiology as it induces the expression of both immune activating and inhibitory proteins. 6,7 The precise effects of IFN␥ on determining the phenotype of ECs may depend on its cooperative signaling with other cytokines or the temporal regulation of target genes. Overall, understanding the role of immune regulatory proteins on the surface of ECs is important for providing insight into the regulation of immune responses. Conclusion-CD155Activation of CD8 T cells by allogeneic human ECs induces proliferation, IFN␥ secretion, and the expression of cytotoxic effector proteins, such as granzyme B. 8 These properties of activated CD8 T cells play a pathological role in allograft rejection. 9 Also, IFN␥ production from activated T cells within arteries drives pathological smooth muscle cell proliferation in the arterial intima and vasomotor dysfunction, thereby contributing to the pathogenesis of some vascular diseases. 10 Interleukin (IL)-10 is also produced by effector T cells that secrete IFN␥, as well as by some induced regulatory T cells. Expression of IL-10 in effector T cells develops late after T-cell activation, correlates with increased i...

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“…In thymus medulla, the single positive T-cells that show affinity to self-antigens presented on MHC molecules receive pro-apoptotic signal (Le Borgne et al 2009, Qiu et al 2010, Atibalentja et al 2009, McCaughtry et al 2008. This elimination of auto-reactive T-cells is called negative selection, and its main purpose is to prevent the development of autoimmunity.…”

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confidence: 99%

Nuclear entropy, angular second moment, variance and texture correlation of thymus cortical and medullar lymphocytes: Grey level co-occurrence matrix analysis

Pantić

Pantic

Paunovic

et al. 2013

An. Acad. Bras. Ciênc.

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Grey level co-occurrence matrix analysis (GLCM) is a well-known mathematical method for quantification of cell and tissue textural properties, such as homogeneity, complexity and level of disorder. Recently, it was demonstrated that this method is capable of evaluating fine structural changes in nuclear structure that otherwise are undetectable during standard microscopy analysis. In this article, we present the results indicating that entropy, angular second moment, variance, and texture correlation of lymphocyte nuclear structure determined by GLCM method are different in thymus cortex when compared to medulla. A total of 300 thymus lymphocyte nuclei from 10 one-month-old mice were analyzed: 150 nuclei from cortex and 150 nuclei from medullar regions of thymus. Nuclear GLCM analysis was carried out using National Institutes of Health ImageJ software. For each nucleus, entropy, angular second moment, variance and texture correlation were determined. Cortical lymphocytes had significantly higher chromatin angular second moment (p < 0.001) and texture correlation (p < 0.05) compared to medullar lymphocytes. Nuclear GLCM entropy and variance of cortical lymphocytes were on the other hand significantly lower than in medullar lymphocytes (p < 0.001). These results suggest that GLCM as a method might have a certain potential in detecting discrete changes in nuclear structure associated with lymphocyte migration and maturation in thymus.

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CD155 Is Involved in Negative Selection and Is Required To Retain Terminally Maturing CD8 T Cells in Thymus

Abstract: Material

Cited by 14 publications

References 57 publications

CD155 (PVR/Necl5) Mediates a Costimulatory Signal in CD4+ T Cells and Regulates Allergic Inflammation

CD155 (PVR/Necl5) Mediates a Costimulatory Signal in CD4+ T Cells and Regulates Allergic Inflammation

CD155 on Human Vascular Endothelial Cells Attenuates the Acquisition of Effector Functions in CD8 T Cells

Nuclear entropy, angular second moment, variance and texture correlation of thymus cortical and medullar lymphocytes: Grey level co-occurrence matrix analysis

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