CD200 and membrane protein interactions in the control of myeloid cells

Barclay, A. Neil; Wright, Gavin J.; Brooke, Gary; Brown, Marion H.

doi:10.1016/s1471-4906(02)02223-8

Cited by 381 publications

(346 citation statements)

References 56 publications

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“…Interestingly, CD200 is an immunomodulatory molecule potentially suppressing inflammation and autoimmune destruction, as well as osteoclast differentiation. (38)(39)(40) The juvenile growth plate is a continuously remodeling tissue in which hypertrophic cells secrete not only matrix metalloproteinases for degradation of the cartilage matrix (34,41) but also vascular endothelial growth factor that induces invasion of bone marrow sprouts. (42) While a portion of the hypertrophic chondrocytes undergoes apoptosis, endochondral bone is deposited on the scaffold of residual spicules of hypertrophic cartilage.…”

Section: Discussionmentioning

confidence: 99%

Sorting of growth plate chondrocytes allows the isolation and characterization of cells of a defined differentiation status

Belluoccio¹,

Etich²,

Rosenbaum³

et al. 2010

Journal of Bone and Mineral Research

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Axial growth of long bones occurs through a coordinated process of growth plate chondrocyte proliferation and differentiation. This maturation of chondrocytes is reflected in a zonal change in gene expression and cell morphology from resting to proliferative, prehypertrophic, and hypertrophic chondrocytes of the growth plate followed by ossification. A major experimental limitation in understanding growth plate biology and pathophysiology is the lack of a robust technique to isolate cells from the different zones, particularly from small animals. Here, we report on a new strategy for separating distinct chondrocyte populations from mouse growth plates. By transcriptome profiling of microdissected zones of growth plates, we identified novel, zone-specific cell surface markers and used these for flow cytometry and immunomagnetic cell separation to quantify, enrich, and characterize chondrocytes populations with respect to their differentiation status. This approach provides a novel platform to study cartilage development and characterize mouse growth plate chondrocytes to reveal unique cellular phenotypes of the distinct subpopulations within the growth plate. ß

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Section: Discussionmentioning

confidence: 99%

Sorting of growth plate chondrocytes allows the isolation and characterization of cells of a defined differentiation status

Belluoccio¹,

Etich²,

Rosenbaum³

et al. 2010

Journal of Bone and Mineral Research

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“…M141R is a membrane-associated protein which shares significant amino acid similarity to the family of cellular CD200 (OX-2) proteins, responsible for regulating the activities of myeloid lineage cells [4]. A number of features present in cellular CD200/OX-2 are also found in M141R, including the C-terminal transmembrane domain, two highly conserved cysteine residues involved in the formation of the V-like immunoglobin domain and several critical residues invariably conserved in immunoglobin domains [11].…”

Section: Leukocyte Activation: M141rmentioning

confidence: 99%

Myxoma virus in the European rabbit: interactions between the virus and its susceptible host

2007

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-Myxoma virus (MV) is a poxvirus that evolved inSylvilagus lagomorphs, and is the causative agent of myxomatosis in European rabbits (Oryctolagus cuniculus). This virus is not a natural pathogen of O. cuniculus, yet is able to subvert the host rabbit immune system defenses and cause a highly lethal systemic infection. The interaction of MV proteins and the rabbit immune system has been an ideal model to help elucidate host/poxvirus interactions, and has led to a greater understanding of how other poxvirus pathogens are able to cause disease in their respective hosts. This review will examine how MV causes myxomatosis, by examining a selection of the identified immunomodulatory proteins that this virus expresses to subvert the immune and inflammatory pathways of infected rabbit hosts. myxoma virus / immunomodulation / poxvirus / Oryctolagus cuniculus

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“…As an example a membrane glycoprotein CD200 (FC −28.3; Panel A, group 6) expressed on a broad rage of cell types was down-regulated during beta cell maturation. CD200 is known to have an inhibiting effect on activation of macrophages and myeloid cells through interaction with the CD200 receptor on these cells (reviewed in [46,47]). Indeed using CD200 knock-out mice, an accelerated induction of the autoimmune diseases experimental allergic encephalomyelitis and collagen-induced arthritis has been demonstrated.…”

Section: Glycolysis and Energy Generationmentioning

confidence: 99%

Gene expression profiles during beta cell maturation and after IL-1? exposure reveal important roles of Pdx-1 and Nkx6.1 for IL-1? sensitivity

et al. 2004

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Aim/hypothesis. Maturation of the beta cells in the islets of Langerhans is dependent upon sequential activation of different transcription factors such as Pdx-1 and Nkx6.1. This maturation is associated with an acquired sensitivity to cytokines and may eventually lead to type 1 diabetes. The aims of this study were to characterise changes in mRNA expression during beta cell maturation as well as after interleukin-1β (IL-1β) exposure. Methods. Transcriptome analyses were performed on two phenotypes characterised as a glucagon-producing pre-beta-cell phenotype (NHI-glu), which matures to an IL-1β-sensitive insulin-producing beta cell phenotype (NHI-ins). Beta cell lines over-expressing Pdx-1 or Nkx6.1, respectively, were used for functional characterisation of acquired IL-1β sensitivity.Results. During beta cell maturation 98 fully annotated mRNAs changed expression levels. Of these, 50 were also changed after 24 h of IL-1β exposure. In addition, 522 and 197 fully annotated mRNAs, not affected by maturation, also changed expression levels following IL-1β exposure of the beta cell and the prebeta-cell phenotype, respectively. Beta cell maturation was associated with an increased expression of Nkx6.1, whereas both Pdx-1 and Nkx6.1 expression were decreased following IL-1β exposure. Over-expression of Nkx6.1 or Pdx-1 in cell lines resulted in a significantly increased sensitivity to IL-1β. Conclusions/interpretation. These results suggest that the final beta cell maturation accompanied by increased IL-1β sensitivity is, in part, dependent upon the expression of genes regulated by Pdx-1 and Nkx6.1. Future classification of the genes regulated by these transcription factors and changed during beta cell maturation should elucidate their role in the acquired sensitivity to IL-1β and may be helpful in identifying new targets for intervention/prevention strategies.

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CD200 and membrane protein interactions in the control of myeloid cells

Cited by 381 publications

References 56 publications

Sorting of growth plate chondrocytes allows the isolation and characterization of cells of a defined differentiation status

Sorting of growth plate chondrocytes allows the isolation and characterization of cells of a defined differentiation status

Myxoma virus in the European rabbit: interactions between the virus and its susceptible host

Gene expression profiles during beta cell maturation and after IL-1? exposure reveal important roles of Pdx-1 and Nkx6.1 for IL-1? sensitivity

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