CD24 and Galectin-1 Expressions in Gastric Adenocarcinoma and Clinicopathologic Significance

Abstract

CD24 and galectin-1 expression in gastric adenocarcinoma and their clinicopathologic significance remained largely unknown. We aimed to evaluate expressions and staining intensities of CD24 and galectin-1 in gastric adenocarcinoma and to investigate the interrelation with clinicopathologic parameters including survival. 93 cases with gastric adenocarcinoma were reevaluated histopathologically and immunohistochemistry was performed with antibodies against CD24 and galectin-1. Staining intensities of both markers in tumor cells and staining intensity of galectin-1 in tumor-associated stromal cells were scored semiquantitatively. The relationship between expression and staining intensity of CD24 and galectin-1 and clinicopathologic variables were assessed. CD24 staining intensity was associated with lymphovascular invasion (p = 0.007), serosal invasion (p = 0.001), stage (p = 0.001) and lymph node metastasis (p = 0.005). Galectin-1 staining intensity in tumor-associated stromal cells was associated with tumor location (p = 0.031), lymphovascular invasion (p = 0.001), perineural invasion (p = 0.001), serosal invasion (p = 0.001), differentiation (p = 0.003), stage (p = 0.001) and lymph node metastasis (p = 0.001). Staining intensity of CD24 (p = 0.019) and gal-1 (p = 0.018) were associated with patient survival. Staining intensity of CD24 in tumor cells and galectin-1 in tumor-associated stromal cells were related with certain clinicopathologic variables. Our findings suggest that these markers are independent prognostic indicators of poor survival and may serve as useful targets for novel therapies.

CD24 is expressed in gastric parietal cells and regulates apoptosis and the response toHelicobacter felisinfection in the murine stomach

“…Although CD24 is known to be overexpressed in gastric adenocarcinoma (4) and gastric cancer stem cells (39), previous immunohistochemical studies have not demonstrated expression in normal human gastric epithelium (6).…”

“…CD24 IS A HEAVILY GLYCOSYLATED glycosyl phosphatidylinositollinked glycoprotein, and increased expression of CD24 has been associated with poor prognosis (4,6,37), tumor progression (1,14), and metastasis in several gastrointestinal (GI) cancers. The function of CD24 in the GI tract, however, remains largely unknown, although its roles in regulating immune cell apoptosis (5,33) and differentiation (16,17) as well as cell migration (27) have been well characterized in other tissues.…”

Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer

“…However, Okada et al [23] demonstrated that reduced galectin-3 expression was associated with lymph node metastasis, advanced stage, and tumor differentiation in GC. It was reported that galectin-1 staining intensity in tumor-associated stromal cells was associated with stage and lymph node metastasis [24]. In regard to galectin-2, Takaishi and Wang [21] confirmed reduced expression of the galectin-2 gene in GC lesions in Helicobacter-infected mice.…”

A galektin-1 alapvető faktor a csontvelő eredetű mesenchymalis őssejtek tumor fejlődést serkentő hatásában

“…emlőtumor sztróma sejtekben [105,115,116], tumor endotéliumban [117]. Az irodalmi adatok szerint a Gal-1 mennyisége a tumor metasztatikus hajlamával és a betegség rossz prognózisával jelentős korrelációt mutat [102].…”

“…Az emlő tumorral asszociált sztróma sejtekben termelődő Gal-1 szintje egyenesen arányos a tumor invazivitásával és a nyirokcsomó áttétek gyakoriságával [105]. A saját nem közölt eredményeink és mások adatai [105,115,116] …”