CD3+/CD4+/CD5+/CD8+/CD21+/CD34-/CD45-/CD79a-/TCRαβ+/TCRγδ-/MHCII+ T-zone lymphoma in a dog with generalized lymphadenopathy: a case report

Abstract

Canine T-zone lymphoma (TZL) is a mature T-cell lymphoma in dogs. The diagnosis and sub-classification are impossible without biopsy or immunophenotyping by flow cytometry. An 11-year-old, spayed, female Golden Retriever presented with lymph node enlargement. Clinical examination was consistent with canine multicentric lymphoma. However, immunophenotyping revealed positive for CD3, CD4, CD5, CD8, CD21, TCRαβ, and MHCII but negative for CD34, CD45, CD79a, and TCRγδ. Histopathology revealed lymphocytes expanding to the cortex-preserving architecture and thinning of the nodal capsule, and CD3 positive but PAX-5 negative. Owing to the indolent nature of TZL, careful monitoring approach without clinical intervention was utilized.

Treatment of canine CD3+/CD21+/CD45- T-zone lymphoma with chlorambucil and prednisolone in two dogs: case reports

“…Immunophenotyping is critical for the diagnosis of TZL. The characteristics of the TZL immunophenotype have been well described, including the loss of CD45 expression [1,[3][4][5][6]. In our cases, the results of immunophenotyping were consistent with those of TZL, and small-to intermediate-sized lymphocytes were found on cytological examination.…”

“…Large cell types are known to have an aggressive clinical course, while small cell T-cell lymphoma has an indolent form referred to as T-zone lymphoma (TZL) [1][2][3][4]. Immunophenotyping using flow cytometric analysis (FCA) or immunohistochemistry is helpful in the diagnosis of TZL [4][5][6].…”

Comparative analysis of the aberrant immunophenotype and clinical characteristics in dogs with lymphoma: a study of 27 cases

“…The antibodies used in this study were based on previous studies ( 23 ). Multi-color flow cytometric analysis was conducted to evaluate the contemporary expression of different antigens within the same cellular population.…”

“…All samples were analyzed within 24 h of collection by the same operator, and those with ambiguous diagnoses, low cellularity, or low viability were excluded. The antibodies used in this study were based on previous studies (23). Multi-color flow cytometric analysis was conducted to evaluate the contemporary expression of different antigens within the same cellular population.…”

Peritoneal lymphomatosis with pleural and peritoneal effusions in a dog: a case report

“…Peripheral blood analysis showed no significant findings, except for neutrophilia with left shift (segmented neutrophil: 29.39 × 10 9 /L, band neutrophils: 2,942 cells/μL) and hypoalbuminemia (2.1 g/ dL). Flow cytometry and clonality tests were used to verify the immunophenotype of malignant round cells, as described previously [4].…”