CD34-positive cells in human umbilical cord blood express nerve growth factor and its specific receptor TrkA

Inhibition of Abl tyrosine kinase enhances nerve growth factor-mediated signaling in Bcr–Abl transformed cells via the alteration of signaling complex and the receptor turnover

“…All primary CD34 þ CML cells were clearly shown to be TrkA positive (Figure 8b). In agreement with previous work (Bracci-Laudiero et al, 2003), CD34 þ cells from peripheral blood from healthy donors were stained weakly by TrkA-specific antibody (data not shown). We then performed a colony-forming assay in the presence of interleukin-3 (10 ng/ml) and granulocyte-macrophage colony-stimulating factor (20 ng/ml) with and without imatinib and/or NGF (50 or 200 ng/ml).…”

“…The difference may be explained by the fact that TrkAnegative cell lines, BV-173 and CML-T1 are CD34À and derived from patients in blast crisis, whereas all primary CD34 þ cells were derived from patients with CML in early chronic phase. In addition, CD34 þ cells from healthy donors also express TrkA (Bracci-Laudiero et al, 2003) and NGF is normally expressed by bone marrow stromal cells (Simone et al, 1999), suggesting that NGF plays a role in maintenance of CD34 þ cells Figure 4 Imatinib treatment restores TrkA-mediated phosphorylation of SHC. (a) The complex of CrkL with Bcr-Abl, GAB2 and SHIP2 in K562 cells is not detectable after imatinib treatment.…”

Cotransplantation with specific populations of spina bifida bone marrow stem/progenitor cells enhances urinary bladder regeneration

“…We postulate that the increased number of blood vessels in MSC/CD34 + grafts (assumingly allowing for greater blood flow to grafted areas) expedited the growth and development of peripheral nerves by providing key nerve-promoting factors. CD34 + HSPCs have also been shown to express a variety of neural proteins with an overlap of hematopoietic and neuropoietic molecular signatures including nerve growth factor, its receptor TrkA, and dopamine receptors DR3 and DR5 and also possess the ability to transdifferentiate into cells of neural lineage (30)(31)(32)(33). Dopamine has been determined to be a key molecule in neurite outgrowth, and its interaction with CD34 + HSPCs may have contributed to the peripheral nerve regeneration at the early time point of these experiments (34).…”

Infusion of Human Umbilical Cord Blood Cells in a Rat Model of Stroke Dose-Dependently Rescues Behavioral Deficits and Reduces Infarct Volume

“…The mononuclear cord blood cells have been shown to express growth factors, such as nerve growth factor, 25 and some investigators have hypothesized the major role of endogenous neuroprotective factors in the HUCB-induced ischemic brain recovery. 26 Regardless of the mechanism of action, the clinical relevance of this HUCBC-based therapeutic option for ischemic stroke is obvious, although additional work is needed before use of HUCBC in the clinical setting.…”