CD34<sup>+</sup>/CD90<sup>+</sup> cells infused best predict late haematopoietic reconstitution following autologous peripheral blood stem cell transplantation

Sumikuma, Toshiya; Shimazaki, Chihiro; Inaba, Tohru; Ochiai, Naoya; Okano, Akira; Hatsuse, Mayumi; Ashihara, Eishi; Nakagawa, Masao

doi:10.1046/j.1365-2141.2002.03373.x

Cited by 24 publications

(29 citation statements)

References 31 publications

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“…These Ccsp + cells were found in the plastic-adherent fraction and expressed some markers used to characterize mesenchymal cells (22). However, CD90 and CD105 are also known to be expressed in CD45 + hematopoietic precursors (23,24). Several reports have suggested that there is a developmental relationship between hematopoietic stem cells and their mesenchymal progenitors, in which nonadherent hematopoietic cells that can reconstitute the blood have also been shown to give rise to stromal cells (25,26).…”

Section: Discussionmentioning

confidence: 99%

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Wong¹,

Keating²,

Lu³

et al. 2009

J. Clin. Invest.

121

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The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation of adherent human and murine bone marrow cells that expresses Clara cell secretory protein (CCSP) was identified using flow cytometry. When cultured at the air-liquid interface in ex vivo cultures, Ccsp + cells expressed type I and type II alveolar markers as well as basal cell markers and active epithelial sodium channels. Ccsp + cells preferentially homed to naphthalenedamaged airways when delivered transtracheally or intravenously, with the former being more efficient than the latter. Interestingly, naphthalene-induced lung damage transiently increased Ccsp expression in bone marrow and peripheral circulation. Furthermore, lethally irradiated Ccsp-null mice that received tagged wild-type bone marrow contained donor-derived epithelium in both normal and naphthalene-damaged airways. This study therefore identifies what we believe to be a newly discovered cell in the bone marrow that might have airway reconstitution potential in the context of cell-based therapies for lung disease. Additionally, these data could reconcile previous controversies regarding the contribution of bone marrow to lung regeneration.

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Section: Discussionmentioning

confidence: 99%

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Wong¹,

Keating²,

Lu³

et al. 2009

J. Clin. Invest.

121

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“…CD90 is of particular interest, because its expression is associated with a subset of CD34 + stem cells that are predictive of successful hematopoietic engraftments evaluated in cancer patients undergoing peripheral blood stem cell transplantations (35). The expression of CD90 on EPC and pericytes may be indicative of Figure 3.…”

Section: Discussionmentioning

confidence: 99%

Pericytes and Endothelial Precursor Cells: Cellular Interactions and Contributions to Malignancy

Bagley¹,

Weber²,

Rouleau³

et al. 2005

Cancer Research

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Tumor vasculature is irregular, abnormal, and essential for tumor growth. Pericytes and endothelial precursor cells (EPC) contribute to the formation of blood vessels under angiogenic conditions. As primary cells in culture, pericytes and EPC share many properties such as tube/network formation and response to kinase inhibitors selective for angiogenic pathways. Expression of cell surface proteins including plateletderived growth factor receptor, vascular cell adhesion molecule, intercellular adhesion molecule, CD105, desmin, and neural growth proteoglycan 2 was similar between pericytes and EPC, whereas expression of P1H12 and lymphocyte function-associated antigen-1 clearly differentiates the cell types. Further distinction was observed in the molecular profiles for expression of angiogenic genes. Pericytes or EPC enhanced the invasion of MDA-MB-231 breast cancer cells in a coculture assay system. The s.c. coinjection of live pericytes or EPC along with MDA-MB-231 cells resulted in an increased rate of tumor growth compared with coinjection of irradiated pericytes or EPC. Microvessel density analysis indicated there was no difference in MDA-MB-231 tumors with or without EPC or pericytes. However, immunohistochemical staining of vasculature suggested that EPC and pericytes may stabilize or normalize vasculature rather than initiate vasculogenesis. In addition, tumors arising from the coinjection of EPC and cancer cells were more likely to develop lymphatic vessels. These results support the notion that pericytes and EPC contribute to malignancy and that these cell types can be useful as cellbased models for tumor vascular development and selection of agents that may provide therapeutic benefit. (Cancer Res 2005; 65(21): 9741-50)

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“…Three of five subsequent studies analyzing the threshold value for CD34 þ CD90 þ linÀ cells shared these results. 22,26 However, as seen in Table 2 neither the threshold nor the phenotype of cells to predict platelet engraftment was found to be consistent among these trials. Of note, this population must not be confused with MSCs, which are CD34ÀCD90 þ LinÀ.…”

Section: Introductionmentioning

confidence: 96%

“…CD34 þ /90 þ cells were better predictors of engraftment than the other subpopulations analyzed. 26 The number of CD34 þ / CD90 þ cells constitute approximately one-fourth of the total CD34 þ cells and are generally fewer than the CD34 þ /CD133 þ cells, but more highly represented than the CD34 þ /CD38 --cells. CD34 þ /CD90 þ cells are mobilized into blood at a maximum level a few days earlier than peak mobilization of the total CD34 þ cells.…”

Section: Introductionmentioning

confidence: 99%

Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?

Beksaç

Preffer

2011

Bone Marrow Transplant

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In the clinical practice of hematopoietic SCT, the minimum numbers of cells required for a successful engraftment are defined on the basis of their CD45 and CD34 expression profiles. However, the quantity of earlier progenitors or CD34-positive cells at different differentiation stages within stem cell grafts is not generally taken into consideration. During the last decade, various teams have quantified the number of cells expressing various combinations of CD34, CD38, CD133, CD90 co-expression and/or aldehyde dehydrogenase functional capacity using flow cytometry. Some of these studies resulted in the greater appreciation that combinations of these Ags were associated with varied myeloid, erythroid and platelet engraftment rates whereas others showed that the relative absence or presence of these markers could define cells responsible for either short-or long-term engraftment. These findings were also extended to differences between progenitor cell populations found within BM vs peripheral or cord-blood grafts. Cells harvested from donors are also generally frozen and stored; thawed cells have variable levels of viability and functional capacity based on the time tested post thaw, which also can be assessed by flow cytometry. Finally, flow cytometry has the potential for analysis of cells carrying a mesenchymal stem cell phenotype, which may be quiescent within some of the stem cell products. This review will address the need for stem cell subpopulation quantification and summarize existing published data to identify some Ags and functional characteristics that can be applicable to daily clinical practice.

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CD34⁺/CD90⁺ cells infused best predict late haematopoietic reconstitution following autologous peripheral blood stem cell transplantation

Cited by 24 publications

References 31 publications

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Pericytes and Endothelial Precursor Cells: Cellular Interactions and Contributions to Malignancy

Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?

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CD34+/CD90+ cells infused best predict late haematopoietic reconstitution following autologous peripheral blood stem cell transplantation

Cited by 24 publications

References 31 publications

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium

Pericytes and Endothelial Precursor Cells: Cellular Interactions and Contributions to Malignancy

Is it time to revisit our current hematopoietic progenitor cell quantification methods in the clinic?

Contact Info

Product

Resources

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CD34⁺/CD90⁺ cells infused best predict late haematopoietic reconstitution following autologous peripheral blood stem cell transplantation