CD36 is expressed in a defined subpopulation of neurons in the olfactory epithelium

Xavier, André Machado; Ludwig, Raissa G.; Nagai, Maíra H.; Almeida, Tiago Jonas de; Watanabe, Hebe Mizuno; Hirata, Marcio Yukio; Rosenstock, Tatiana R.; Papes, Fábio; Malnic, Bettina; Glezer, Isaías

doi:10.1038/srep25507

Cited by 34 publications

(29 citation statements)

References 56 publications

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“…On the basis of gas chromatography, the puriimportant for interactions with the binding site (5). Recently, we and others have demonstrated that CD36 is produced by distinct types of olfactory sensory neurons and is abundantly present on the surface of the olfactory epithelium (i.e., on the ciliary layer) in mice (14,18,31). In addition, another series of studies using Drosophila melanogaster have illustrated that a sensory neuron membrane protein, a CD36 homolog in the insect, localizes on the dendrites of specific olfactory neurons to mediate sensing of volatile pheromones, including (Z)-11-octadecenyl acetate (3,6).…”

Section: Preparation Of Test Compoundsmentioning

confidence: 99%

Identification of the odor-active volatile compound (Z,Z)-4,7-tridecadienal as a potential ligand for the transmembrane receptor CD36

et al. 2016

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Cluster of differentiation 36 (CD36) is a broadly expressed transmembrane protein that has multiple ligands, including oxidized low-density lipoproteins. We found recently that CD36 is expressed in olfactory sensory neurons and postulated that it plays a role in the detection of distinct odorants in the nasal cavity. To date, however, there have been few examples of attempts to identify CD36-recognizable odorants. In this study, by an in vitro assay using a peptide mimic of the receptor, we provided evidence that CD36 recognizes (Z,Z)-4,7-tridecadienal, an odor-active volatile compound that is known to occur in Katsuobushi (dried, fermented, and smoked skipjack tuna commonly used in Japanese cuisine as a seasoning) and in the preorbital secretion of male oribi. In addition, by comparing the data with those of its related compounds, we provided information on the structural requirements of (Z,Z)-4,7-tridecadienal for recognition by CD36. For instance, we showed that flexible rotation around the C2-C3 bond of the volatile may be of importance in gaining access to CD36. Identification of (Z,Z)-4,7-tridecadienal as the ligand prompts us to hypothesize that CD36 could participate in the control of distinct mammalian behaviors (e.g., food selection) through its ability to recognize specific odorants in the environment.

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Section: Preparation Of Test Compoundsmentioning

confidence: 99%

Identification of the odor-active volatile compound (Z,Z)-4,7-tridecadienal as a potential ligand for the transmembrane receptor CD36

et al. 2016

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“…A novel finding of our studies using the CD36 peptides was that aliphatic aldehydes such as tetradecanal, a distinct class of volatile odorants, were capable of inhibiting fl-oxLDL binding to the mimics (i.e., were identified as potential CD36 ligands) (32,33,35). Furthermore, others and we have discovered that CD36 is expressed by a population of olfactory sensory neurons and is abundantly present in the olfactory ciliary layer in mice (17,22,38). These recent findings led us to propose that CD36 participates in the capture and detection of specific odorants in the nasal cavity of mammals.…”

Section: Preparation Of Mimic Peptides For Class B Scavengermentioning

confidence: 94%

“…Certain sensory neuron membrane proteins (SNMPs), the insect homologues of mammalian class B scavenger receptors, are located in supporting cells around the olfactory sensory neurons in organisms (4,25). CD36 is also known to be present on or around the microvilli of olfactory supporting cells (17,38). As for SNMPs, SNMP-2 (in Heliothis viresces) is assumed to participate in quick pheromone clearance of the cuticular sensilla lymph to allow highly sensitive olfactory detection (6).…”

Section: Discussionmentioning

confidence: 99%

“…CD36 is produced by a population of olfactory sensory neurons, and is transported to and accumulates in the cilia, the first line for olfactory percep-tion of odorants (17,22,38). The most likely role of the receptor at this site has been postulated to serve as an obligate transmembrane partner for G-protein-coupled odorant receptors, facilitating the recognition of specific odorants (4,12,38). In contrast to CD36, there was no apparent evidence for the existence of SR-B1 in the somata of olfactory sensory neurons (Fig.…”

Section: Discussionmentioning

confidence: 99%

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A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal cavity

et al. 2018

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Class B scavenger receptors, scavenger receptor B1 (SR-B1) and cluster of differentiation 36 (CD36), are broadly expressed cell-surface proteins and are believed to serve as multifaceted players in lipid and lipoprotein metabolism in mammals. Because of its ability to recognise distinct odour-active volatile compounds and its presence in murine olfactory epithelium, CD36 has recently emerged as a participant in the detection of odorants within the nasal cavity. However, there have been no attempts to assess whether SR-B1 has such a role. In this study, we performed a cell-free in-vitro assay utilising a peptide mimic of the receptor, and demonstrated that SR-B1 could recognise aliphatic aldehydes (e.g., tetradecanal), a distinct class of volatile odorants, as potential ligands. By reverse transcription-polymerase chain reaction and western immunoblot analyses, we detected the expression of SR-B1 mRNA and protein, respectively, in mouse olfactory tissue. Finally, we immunohistochemically mapped the distribution of SR-B1 in the surface layer of olfactory epithelium in vivo, which is the first line of odorant detection. These findings uncover a novel role for SR-B1 as a contributor to the capture of specific odorants in the nasal cavity of mammals.Class B scavenger receptors are cell-surface proteins, characterised by two predicted transmembrane spans, broad expression patterns and the ability to recognise diverse ligands (13). Of these, scavenger receptor B1 (SR-B1) was initially identified as comprising 509 amino-acid residues (5). An earlier study has showed that SR-B1 is expressed in several tissues including the liver and adipose tissues, and can serve as a receptor for low-density lipoprotein (LDL), acetylated LDL, oxidised LDL (oxLDL) and maleylated bovine serum albumin (BSA) (2). Later, SR-B1 was found to recognise several other substances such as high-density lipoprotein (HDL) (1) and anionic phospholipids (15). These findings suggested that SR-B1 primarily participates in lipid and lipoprotein metabolism in internal organs (36). As studied extensively, it functions as a physiologically relevant HDL receptor to mediate the selective delivery of HDL-cholesterol (i.e., HDL-cholesteryl ester) to the liver and steroidogenic tissues (1,13,20). Another member of class B scavenger receptors, cluster of differentiation 36 (CD36), which comprises 472 amino-acid residues, is also a multifaceted and multifunctional player in lipid and lipoprotein metabolism (13,27,28). CD36 is known to share with SR-B1 the ability to recognise distinct ligands,

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“…Subsequently, a vessel containing a sheet of filter recent studies demonstrated a novel role of CD36-the role in the olfactory system. This is exemplified by (i) the localisation of the receptor on the cilia of olfactory sensory neurons in the murine main olfactory epithelium (19), (ii) a weaker response to oleic acid in the olfactory sensory neurons of CD36-knockout mice compared with their wild-type littermates (21) and (iii) a reduction of preference for a lipid mixture odour through dysfunction of CD36 in mice (29). However, there have been no acoustic and ethological analyses of mammalian behaviour addressing the involvement of CD36 in the olfactory perception of specific odour-active volatile compounds.…”

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confidence: 99%

CD36 involvement in the olfactory perception of oleic aldehyde, an odour-active volatile compound, in mice

et al. 2017

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Cluster of differentiation 36 (CD36) is a broadly expressed transmembrane receptor that has multiple ligands. It has been found to occur abundantly on the surface of the olfactory epithelium in mice and postulated to play a role in mammalian olfaction. However, there have been no ethological analyses of the mammalian behaviour showing CD36 involvement in the olfactory perception of a distinct odour-active volatile compound. In this study, we aimed to assess whether mammals perceive oleic aldehyde, an odour-active volatile that serves as a potential CD36 ligand, and if so, whether CD36 is involved in the sensing by following measurements using CD36-knockout mice and their wild-type littermates. In a two-bottle choice test, wild-type mice, but not CD36-knockout mice, discriminated a sucrose solution containing oleic aldehyde from the sucrose solution alone. To assess the importance of the olfactory system in the oleic aldehyde perception, we conducted an exploration test where the animals could rely primarily on the odour of test volatiles for recognition. We found that the wild-type, but not CD36-knockout mice, were aware of the compound. Our results provide behavioural evidence that CD36 plays a role in the perception of specific odour-active volatile compounds in the nasal cavity.Chemosensory systems are responsible for the detection of soluble and volatile chemicals. The two chemosensory systems in mammals, gustatory and olfactory systems, distinguish nutritive compounds from harmful ones within the oral and nasal cavities (10). The sense of smell is particularly important for avoiding harmful chemicals in foods prior to ingestion (7). The olfactory perception starts from the binding of odorants to specific G-protein-coupled receptors on olfactory sensory neurons located within the olfactory epithelium of the nasal cavity (5). However, for many odorants, the precise mechanisms of olfactory detection in mammals remain to be studied. Cluster of differentiation 36 (CD36) is a membrane-bound receptor with two transmembrane spans that is produced and expressed by a variety of sites in the body of vertebrates (23). The receptor is involved in lipid binding and uptake within tissues, such as the small intestinal, adipose and muscle tissues (6). CD36 has also been postulated to play roles in the gustatory system. Indeed, CD36 has been shown to mediate the detection of long-chain fatty acids in the oral cavity, driving the consumption of the lipid species in rodents (12, 16). More

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CD36 is expressed in a defined subpopulation of neurons in the olfactory epithelium

Cited by 34 publications

References 56 publications

<b>Identification of the odor-active volatile compound (</b><i><b>Z</b></i><b>,</b><i><b>Z</b></i><b>)-4,7-tridecadienal as a potential ligand for the transmembrane receptor </b><b>CD36 </b>

<b>Identification of the odor-active volatile compound (</b><i><b>Z</b></i><b>,</b><i><b>Z</b></i><b>)-4,7-tridecadienal as a potential ligand for the transmembrane receptor </b><b>CD36 </b>

<b>A novel role for scavenger receptor B1 as a contributor to the capture of specific volatile odorants in the nasal </b><b>cavity </b>

<b>CD36 involvement in the olfactory perception of oleic aldehyde, an odour-active volatile compound, in</b><b> mice </b>

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